Gene description for Agrn
Gene name agrin
Gene symbol Agrn
Other names/aliases Agrin
nmf380
Species Mus musculus
 Database cross references - Agrn
ExoCarta ExoCarta_11603
Vesiclepedia VP_11603
Entrez Gene 11603
 Agrn identified in sEVs derived from the following tissue/cell type
Colon cancer cells 37309723    
Fibroblasts 23260141    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Agrn
Molecular Function
    dystroglycan binding GO:0002162 ISO
    dystroglycan binding GO:0002162 ISS
    extracellular matrix structural constituent GO:0005201 RCA
    calcium ion binding GO:0005509 IEA
    calcium ion binding GO:0005509 ISO
    calcium ion binding GO:0005509 ISS
    protein binding GO:0005515 IPI
    acetylcholine receptor regulator activity GO:0030548 IDA
    sialic acid binding GO:0033691 ISS
    chondroitin sulfate binding GO:0035374 IDA
    BMP binding GO:0036122 ISO
    ATPase inhibitor activity GO:0042030 IDA
    laminin binding GO:0043236 IEA
    heparan sulfate proteoglycan binding GO:0043395 IDA
    heparan sulfate proteoglycan binding GO:0043395 ISS
    transmembrane transporter binding GO:0044325 IPI
    receptor ligand activity GO:0048018 IDA
    transforming growth factor beta binding GO:0050431 ISO
Biological Process
    regulation of protein phosphorylation GO:0001932 ISO
    positive regulation of protein phosphorylation GO:0001934 IDA
    positive regulation of protein phosphorylation GO:0001934 ISO
    plasma membrane organization GO:0007009 IMP
    enzyme-linked receptor protein signaling pathway GO:0007167 IDA
    enzyme-linked receptor protein signaling pathway GO:0007167 IGI
    enzyme-linked receptor protein signaling pathway GO:0007167 IMP
    chemical synaptic transmission GO:0007268 IDA
    chemical synaptic transmission GO:0007268 ISO
    synapse assembly GO:0007416 ISO
    neuromuscular junction development GO:0007528 IBA
    neuromuscular junction development GO:0007528 IGI
    behavior GO:0007610 IGI
    circadian rhythm GO:0007623 IGI
    protein transport GO:0015031 IDA
    cell differentiation GO:0030154 IEA
    positive regulation of protein binding GO:0032092 IDA
    positive regulation of Rac protein signal transduction GO:0035022 IDA
    positive regulation of Rac protein signal transduction GO:0035022 IGI
    regulation of GTPase activity GO:0043087 IDA
    receptor clustering GO:0043113 IBA
    receptor clustering GO:0043113 IDA
    receptor clustering GO:0043113 IEA
    receptor clustering GO:0043113 IGI
    receptor clustering GO:0043113 IMP
    receptor clustering GO:0043113 ISO
    receptor clustering GO:0043113 ISO
    positive regulation of GTPase activity GO:0043547 ISS
    positive regulation of synaptic assembly at neuromuscular junction GO:0045887 IMP
    positive regulation of transcription by RNA polymerase II GO:0045944 IDA
    positive regulation of transcription by RNA polymerase II GO:0045944 ISO
    positive regulation of peptidyl-tyrosine phosphorylation GO:0050731 ISO
    regulation of synapse organization GO:0050807 ISO
    positive regulation of filopodium assembly GO:0051491 ISO
    positive regulation of filopodium assembly GO:0051491 ISS
    regulation of cardiac muscle contraction GO:0055117 IDA
    regulation of synaptic activity GO:0060025 IDA
    positive regulation of protein tyrosine kinase activity GO:0061098 IDA
    regulation of microtubule cytoskeleton organization GO:0070507 ISO
    skeletal muscle acetylcholine-gated channel clustering GO:0071340 ISO
    regulation of cardiac muscle cell membrane potential GO:0086036 IDA
    motor neuron apoptotic process GO:0097049 IMP
    regulation of axon guidance GO:1902667 ISO
    negative regulation of sodium ion export across plasma membrane GO:1903277 IDA
    negative regulation of P-type sodium:potassium-exchanging transporter activity GO:1903407 IDA
    positive regulation of skeletal muscle acetylcholine-gated channel clustering GO:1904395 IDA
    positive regulation of skeletal muscle acetylcholine-gated channel clustering GO:1904395 IGI
    positive regulation of skeletal muscle acetylcholine-gated channel clustering GO:1904395 IMP
    positive regulation of protein geranylgeranylation GO:2000541 IMP
    positive regulation of motor neuron apoptotic process GO:2000673 IMP
Subcellular Localization
    extracellular region GO:0005576 IEA
    extracellular region GO:0005576 TAS
    basement membrane GO:0005604 IDA
    basement membrane GO:0005604 ISO
    extracellular space GO:0005615 HDA
    extracellular space GO:0005615 IDA
    extracellular space GO:0005615 IDA
    extracellular space GO:0005615 TAS
    Golgi lumen GO:0005796 TAS
    plasma membrane GO:0005886 IEA
    cell surface GO:0009986 IDA
    cell junction GO:0030054 IEA
    sarcolemma GO:0042383 IDA
    axonal growth cone GO:0044295 ISO
    synapse GO:0045202 EXP
    synapse GO:0045202 IDA
    synapse GO:0045202 IDA
    synapse GO:0045202 ISO
    collagen-containing extracellular matrix GO:0062023 HDA
    collagen-containing extracellular matrix GO:0062023 ISO
    glutamatergic synapse GO:0098978 ISO
 Experiment description of studies that identified Agrn in sEVs
1
Experiment ID 907
MISEV standards
EM
Biophysical techniques
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
HSPA5
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 210
MISEV standards
EM
Biophysical techniques
CD81|FLOT1
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23260141    
Organism Mus musculus
Experiment description Exosomes Mediate Stromal Mobilization of Autocrine Wnt-PCP Signaling in Breast Cancer Cell Migration.
Authors "Luga V, Zhang L, Viloria-Petit AM, Ogunjimi AA, Inanlou MR, Chiu E, Buchanan M, Hosein AN, Basik M, Wrana JL."
Journal name Cell
Publication year 2012
Sample Fibroblasts
Sample name Normal-Fibroblasts (L cells)
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
3
Experiment ID 908
MISEV standards
EM
Biophysical techniques
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
HSPA5
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Agrn
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Cacna1a  
Co-fractionation Mus musculus
2 Tnik  
Affinity Capture-MS Mus musculus
3 Dvl1  
Phenotypic Suppression Mus musculus
4 Dvl2  
Phenotypic Suppression Mus musculus
5 Eed  
Affinity Capture-MS Mus musculus
6 Pde4dip  
Affinity Capture-MS Mus musculus
7 Dvl3  
Phenotypic Suppression Mus musculus
8 PAK1IP1  
Phenotypic Suppression Homo sapiens
9 Pak1  
Phenotypic Suppression Mus musculus
View the network image/svg+xml
 Pathways in which Agrn is involved
No pathways found





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