Gene description for Alcam
Gene name activated leukocyte cell adhesion molecule
Gene symbol Alcam
Other names/aliases AI853494
BEN
CD166
DM-GRASP
MuSC
SC1
Species Mus musculus
 Database cross references - Alcam
ExoCarta ExoCarta_11658
Vesiclepedia VP_11658
Entrez Gene 11658
UniProt Q61490  
 Alcam identified in sEVs derived from the following tissue/cell type
Colon cancer cells 37309723    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Alcam
Molecular Function
    protein binding GO:0005515 IPI
    identical protein binding GO:0042802 IEA
    identical protein binding GO:0042802 ISO
Biological Process
    adaptive immune response GO:0002250 IEA
    cell adhesion GO:0007155 IBA
    cell adhesion GO:0007155 ISS
    heterophilic cell-cell adhesion via plasma membrane cell adhesion molecules GO:0007157 ISO
    heterophilic cell-cell adhesion via plasma membrane cell adhesion molecules GO:0007157 ISS
    axon guidance GO:0007411 IMP
    motor neuron axon guidance GO:0008045 IMP
    retinal ganglion cell axon guidance GO:0031290 ISS
    axon extension involved in axon guidance GO:0048846 ISS
    neuron projection extension GO:1990138 ISS
Subcellular Localization
    immunological synapse GO:0001772 ISO
    immunological synapse GO:0001772 ISS
    plasma membrane GO:0005886 IBA
    plasma membrane GO:0005886 ISO
    plasma membrane GO:0005886 ISS
    external side of plasma membrane GO:0009897 IDA
    axon GO:0030424 IDA
    dendrite GO:0030425 IEA
    T cell receptor complex GO:0042101 ISO
    T cell receptor complex GO:0042101 ISS
    neuronal cell body GO:0043025 IDA
 Experiment description of studies that identified Alcam in sEVs
1
Experiment ID 907
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 908
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Alcam
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Cd6  
Co-fractionation Mus musculus
2 Tmem173  
Proximity Label-MS Mus musculus
3 Col26a1  
Co-fractionation Mus musculus
View the network image/svg+xml
 Pathways in which Alcam is involved
No pathways found





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