Gene description for Atp6v1f
Gene name ATPase, H transporting, lysosomal V1 subunit F
Gene symbol Atp6v1f
Other names/aliases Atp6s14
Species Rattus norvegicus
 Database cross references - Atp6v1f
ExoCarta ExoCarta_116664
Vesiclepedia VP_116664
Entrez Gene 116664
UniProt P50408  
 Atp6v1f identified in sEVs derived from the following tissue/cell type
Reticulocytes 21828046    
 Gene ontology annotations for Atp6v1f
Molecular Function
    proton-transporting ATPase activity, rotational mechanism GO:0046961 IEA
Biological Process
    synaptic vesicle lumen acidification GO:0097401 ISO
Subcellular Localization
    vacuolar proton-transporting V-type ATPase, V1 domain GO:0000221 ISS
    membrane GO:0016020 IBA
    membrane GO:0016020 ISO
    vacuolar proton-transporting V-type ATPase complex GO:0016471 ISO
    clathrin-coated vesicle membrane GO:0030665 IEA
    proton-transporting V-type ATPase complex GO:0033176 ISO
    extrinsic component of synaptic vesicle membrane GO:0098850 EXP
    extrinsic component of synaptic vesicle membrane GO:0098850 IDA
 Experiment description of studies that identified Atp6v1f in sEVs
1
Experiment ID 90
MISEV standards
✔
EM
Biophysical techniques
✔
HSC70|HSP90|TSG101|MHCI
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors "Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E"
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D2 Rep 1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.16-1.21 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
 Protein-protein interactions for Atp6v1f
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.



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