Gene description for Atp6v1f
Gene name ATPase, H transporting, lysosomal V1 subunit F
Gene symbol Atp6v1f
Other names/aliases Atp6s14
Species Rattus norvegicus
 Database cross references - Atp6v1f
ExoCarta ExoCarta_116664
Entrez Gene 116664
UniProt P50408  
 Atp6v1f identified in exosomes derived from the following tissue/cell type
Reticulocytes 21828046    
 Gene ontology annotations for Atp6v1f
Molecular Function
    ATPase activity, coupled to transmembrane movement of ions GO:0042625 IDA
    proton-transporting ATPase activity, rotational mechanism GO:0046961 IEA
Biological Process
    ATP hydrolysis coupled proton transport GO:0015991 IEA
Subcellular Localization
    extracellular exosome GO:0070062 ISO
    proton-transporting V-type ATPase, V1 domain GO:0033180 IEA
    membrane GO:0016020 ISO
    vacuolar proton-transporting V-type ATPase complex GO:0016471 ISO
 Experiment description of studies that identified Atp6v1f in exosomes
1
Experiment ID 90
ISEV standards
✔
EM
EV Biophysical techniques
✔
HSC70|HSP90|TSG101
EV Cytosolic markers
✔
MHCI
EV Membrane markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D2 Rep 1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.16-1.21 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
 Protein-protein interactions for Atp6v1f
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.
 Pathways in which Atp6v1f is involved
PathwayEvidenceSource
Insulin receptor recycling IEA Reactome
Ion channel transport IEA Reactome
Transferrin endocytosis and recycling IEA Reactome





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