Gene description for Oplah
Gene name 5-oxoprolinase (ATP-hydrolysing)
Gene symbol Oplah
Other names/aliases -
Species Rattus norvegicus
 Database cross references - Oplah
ExoCarta ExoCarta_116684
Vesiclepedia VP_116684
Entrez Gene 116684
UniProt P97608  
 Oplah identified in sEVs derived from the following tissue/cell type
Reticulocytes 21828046    
 Gene ontology annotations for Oplah
Molecular Function
    ATP binding GO:0005524 IEA
    hydrolase activity GO:0016787 IEA
    5-oxoprolinase (ATP-hydrolyzing) activity GO:0017168 IBA
    5-oxoprolinase (ATP-hydrolyzing) activity GO:0017168 ISS
    identical protein binding GO:0042802 IEA
    identical protein binding GO:0042802 ISO
Biological Process
    glutathione metabolic process GO:0006749 IBA
Subcellular Localization
    cytosol GO:0005829 IBA
    cytosol GO:0005829 ISS
 Experiment description of studies that identified Oplah in sEVs
1
Experiment ID 95
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|HSP90|HSC70|MHCI
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors "Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E."
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D7 Rep 1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.25 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
 Protein-protein interactions for Oplah
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.



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