Gene description for Pip4k2a
Gene name phosphatidylinositol-5-phosphate 4-kinase, type II, alpha
Gene symbol Pip4k2a
Other names/aliases Pip5k2a
Species Rattus norvegicus
 Database cross references - Pip4k2a
ExoCarta ExoCarta_116723
Vesiclepedia VP_116723
Entrez Gene 116723
UniProt Q9R0I8  
 Pip4k2a identified in sEVs derived from the following tissue/cell type
Reticulocytes 21828046    
 Gene ontology annotations for Pip4k2a
Molecular Function
    ATP binding GO:0005524 IEA
    1-phosphatidylinositol-4-phosphate 5-kinase activity GO:0016308 IEA
    1-phosphatidylinositol-4-phosphate 5-kinase activity GO:0016308 ISO
    1-phosphatidylinositol-4-phosphate 5-kinase activity GO:0016308 ISS
    1-phosphatidylinositol-5-phosphate 4-kinase activity GO:0016309 IBA
    1-phosphatidylinositol-5-phosphate 4-kinase activity GO:0016309 IEA
    1-phosphatidylinositol-5-phosphate 4-kinase activity GO:0016309 ISO
    protein homodimerization activity GO:0042803 IEA
    protein homodimerization activity GO:0042803 ISO
    protein homodimerization activity GO:0042803 ISS
Biological Process
    regulation of autophagy GO:0010506 ISO
    regulation of autophagy GO:0010506 ISS
    megakaryocyte development GO:0035855 IEA
    megakaryocyte development GO:0035855 ISO
    negative regulation of insulin receptor signaling pathway GO:0046627 IEA
    negative regulation of insulin receptor signaling pathway GO:0046627 ISO
    negative regulation of insulin receptor signaling pathway GO:0046627 ISS
    phosphatidylinositol phosphate biosynthetic process GO:0046854 IBA
    autophagosome-lysosome fusion GO:0061909 IEA
    autophagosome-lysosome fusion GO:0061909 ISO
    autophagosome-lysosome fusion GO:0061909 ISS
    vesicle-mediated cholesterol transport GO:0090119 IEA
    vesicle-mediated cholesterol transport GO:0090119 ISO
    vesicle-mediated cholesterol transport GO:0090119 ISS
    1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate biosynthetic process GO:1902635 IEA
    1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate biosynthetic process GO:1902635 ISO
    1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate biosynthetic process GO:1902635 ISS
    positive regulation of autophagosome assembly GO:2000786 IEA
    positive regulation of autophagosome assembly GO:2000786 ISO
Subcellular Localization
    photoreceptor outer segment GO:0001750 IEA
    photoreceptor inner segment GO:0001917 IEA
    nucleus GO:0005634 IEA
    lysosome GO:0005764 IEA
    lysosome GO:0005764 ISO
    lysosome GO:0005764 ISS
    autophagosome GO:0005776 IEA
    autophagosome GO:0005776 ISO
    cytosol GO:0005829 IEA
    cytosol GO:0005829 ISO
    plasma membrane GO:0005886 IBA
    plasma membrane GO:0005886 IEA
    plasma membrane GO:0005886 ISO
 Experiment description of studies that identified Pip4k2a in sEVs
1
Experiment ID 98
MISEV standards
EM
Biophysical techniques
TSG101|HSP90|HSC70|MHCI
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors "Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E."
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D7 Rep 2
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.25 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
 Protein-protein interactions for Pip4k2a
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.



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