Gene description for Vps4a
Gene name vacuolar protein sorting 4a (yeast)
Gene symbol Vps4a
Other names/aliases 4930589C15Rik
AI325971
AW553189
Species Mus musculus
 Database cross references - Vps4a
ExoCarta ExoCarta_116733
Vesiclepedia VP_116733
Entrez Gene 116733
UniProt Q8VEJ9  
 Vps4a identified in exosomes derived from the following tissue/cell type
Colon cancer cells 37309723    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Vps4a
Molecular Function
    hydrolase activity GO:0016787 IEA
    ATPase activity GO:0016887 ISO
    protein binding GO:0005515 IPI
    protein C-terminus binding GO:0008022 ISO
    Rho GTPase binding GO:0017048 ISO
    nucleotide binding GO:0000166 IEA
    ATP binding GO:0005524 ISO
    protein domain specific binding GO:0019904 ISO
Biological Process
    vesicle-mediated transport GO:0016192 ISO
    positive regulation of viral release from host cell GO:1902188 ISO
    regulation of protein localization GO:0032880 ISO
    cell separation after cytokinesis GO:0000920 ISO
    transport GO:0006810 IEA
    ubiquitin-dependent protein catabolic process via the multivesicular body sorting pathway GO:0043162 ISO
    viral release from host cell GO:0019076 ISO
    viral budding via host ESCRT complex GO:0039702 ISO
    intracellular cholesterol transport GO:0032367 ISO
    positive regulation of viral process GO:0048524 IMP
    vacuole organization GO:0007033 IBA
    abscission GO:0009838 ISO
    mitotic metaphase plate congression GO:0007080 ISO
    positive regulation of exosomal secretion GO:1903543 ISO
    ubiquitin-independent protein catabolic process via the multivesicular body sorting pathway GO:0090611 ISO
    cell cycle GO:0007049 IEA
    nucleus organization GO:0006997 ISO
    positive regulation of viral life cycle GO:1903902 ISO
    negative regulation of cytokinesis GO:0032466 ISO
    positive regulation of viral budding via host ESCRT complex GO:1903774 ISO
    vesicle uncoating GO:0072319 ISO
    membrane budding GO:0006900 ISO
    endosomal vesicle fusion GO:0034058 ISO
    endosomal transport GO:0016197 ISO
    protein transport GO:0015031 IEA
    cell division GO:0051301 ISO
    regulation of protein localization to plasma membrane GO:1903076 ISO
Subcellular Localization
    perinuclear region of cytoplasm GO:0048471 ISO
    midbody GO:0030496 ISO
    cytoplasm GO:0005737 ISO
    lysosome GO:0005764 ISO
    plasma membrane GO:0005886 ISO
    endosome GO:0005768 ISO
    late endosome GO:0005770 ISO
    extracellular exosome GO:0070062 ISO
    vacuolar membrane GO:0005774 ISO
    early endosome GO:0005769 ISO
    Flemming body GO:0090543 ISO
    cytosol GO:0005829 ISO
    centrosome GO:0005813 ISO
    spindle pole GO:0000922 ISO
    nucleus GO:0005634 ISO
    ESCRT III complex GO:0000815 ISO
    membrane GO:0016020 IEA
 Experiment description of studies that identified Vps4a in exosomes
1
Experiment ID 907
MISEV standards
✔
EM
EV Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
EV Enriched markers
✔
HSPA5
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S.
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 908
MISEV standards
✔
EM
EV Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
EV Enriched markers
✔
HSPA5
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S.
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Vps4a
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.
 Pathways in which Vps4a is involved
PathwayEvidenceSource
Endosomal Sorting Complex Required For Transport (ESCRT) IEA Reactome





Perform bioinformatics analysis of your extracellular vesicle data set using FunRich, a open access standalone tool. NEW UPDATED VERSION OF FunRich available for download (12/09/2016) from here