Gene description for Vps4a
Gene name vacuolar protein sorting 4a (yeast)
Gene symbol Vps4a
Other names/aliases 4930589C15Rik
AI325971
AW553189
Species Mus musculus
 Database cross references - Vps4a
ExoCarta ExoCarta_116733
Vesiclepedia VP_116733
Entrez Gene 116733
UniProt Q8VEJ9  
 Vps4a identified in sEVs derived from the following tissue/cell type
Colon cancer cells 37309723    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Vps4a
Molecular Function
    DNA clamp loader activity GO:0003689 IEA
    protein binding GO:0005515 IPI
    ATP binding GO:0005524 IEA
    ATP binding GO:0005524 ISO
    ATP hydrolysis activity GO:0016887 IBA
    ATP hydrolysis activity GO:0016887 IEA
    ATP hydrolysis activity GO:0016887 ISO
    small GTPase binding GO:0031267 ISO
    protein-containing complex binding GO:0044877 IEA
    protein-containing complex binding GO:0044877 ISO
    cohesin loader activity GO:0061775 IEA
    chromatin extrusion motor activity GO:0140584 IEA
    ATP-dependent H3-H4 histone complex chaperone activity GO:0140665 IEA
    ATP-dependent H2AZ histone chaperone activity GO:0140849 IEA
Biological Process
    plasma membrane repair GO:0001778 NAS
    chromatin remodeling GO:0006338 IEA
    protein targeting to lysosome GO:0006622 IEA
    protein targeting to lysosome GO:0006622 ISO
    vesicle budding from membrane GO:0006900 IEA
    vesicle budding from membrane GO:0006900 ISO
    autophagy GO:0006914 NAS
    nucleus organization GO:0006997 ISO
    nucleus organization GO:0006997 NAS
    endosome organization GO:0007032 IGI
    vacuole organization GO:0007033 IBA
    mitotic metaphase chromosome alignment GO:0007080 IEA
    mitotic metaphase chromosome alignment GO:0007080 ISO
    abscission GO:0009838 ISO
    vesicle-mediated transport GO:0016192 ISO
    vesicle-mediated transport GO:0016192 ISS
    endosomal transport GO:0016197 IBA
    endosomal transport GO:0016197 ISO
    viral release from host cell GO:0019076 ISO
    nuclear membrane reassembly GO:0031468 NAS
    intracellular cholesterol transport GO:0032367 IEA
    intracellular cholesterol transport GO:0032367 ISO
    negative regulation of cytokinesis GO:0032466 ISO
    negative regulation of cytokinesis GO:0032466 ISS
    regulation of protein localization GO:0032880 ISO
    endosomal vesicle fusion GO:0034058 IEA
    endosomal vesicle fusion GO:0034058 ISO
    multivesicular body assembly GO:0036258 IMP
    multivesicular body assembly GO:0036258 NAS
    viral budding via host ESCRT complex GO:0039702 IEA
    viral budding via host ESCRT complex GO:0039702 ISO
    ubiquitin-dependent protein catabolic process via the multivesicular body sorting pathway GO:0043162 IEA
    ubiquitin-dependent protein catabolic process via the multivesicular body sorting pathway GO:0043162 ISO
    mitotic cytokinesis checkpoint signaling GO:0044878 ISO
    mitotic cytokinesis checkpoint signaling GO:0044878 ISS
    viral budding from plasma membrane GO:0046761 IMP
    viral budding from plasma membrane GO:0046761 ISO
    cell division GO:0051301 ISO
    late endosomal microautophagy GO:0061738 IMP
    late endosome to lysosome transport via multivesicular body sorting pathway GO:0061764 NAS
    midbody abscission GO:0061952 ISO
    midbody abscission GO:0061952 ISS
    midbody abscission GO:0061952 NAS
    multivesicular body sorting pathway GO:0071985 NAS
    vesicle uncoating GO:0072319 IEA
    vesicle uncoating GO:0072319 ISO
    membrane fission GO:0090148 NAS
    ubiquitin-independent protein catabolic process via the multivesicular body sorting pathway GO:0090611 IEA
    ubiquitin-independent protein catabolic process via the multivesicular body sorting pathway GO:0090611 ISO
    autophagosome maturation GO:0097352 NAS
    chromatin looping GO:0140588 IEA
    regulation of protein localization to plasma membrane GO:1903076 IEA
    regulation of protein localization to plasma membrane GO:1903076 ISO
    positive regulation of exosomal secretion GO:1903543 IEA
    positive regulation of exosomal secretion GO:1903543 ISO
    positive regulation of viral budding via host ESCRT complex GO:1903774 IEA
    positive regulation of viral budding via host ESCRT complex GO:1903774 ISO
Subcellular Localization
    spindle pole GO:0000922 IEA
    spindle pole GO:0000922 ISO
    nucleus GO:0005634 ISO
    nuclear pore GO:0005643 NAS
    cytoplasm GO:0005737 IDA
    cytoplasm GO:0005737 ISO
    lysosome GO:0005764 IEA
    lysosome GO:0005764 ISO
    endosome GO:0005768 ISO
    early endosome GO:0005769 IEA
    early endosome GO:0005769 ISO
    late endosome GO:0005770 ISO
    vacuolar membrane GO:0005774 IEA
    vacuolar membrane GO:0005774 ISO
    centrosome GO:0005813 IEA
    centrosome GO:0005813 ISO
    cytosol GO:0005829 IEA
    cytosol GO:0005829 ISO
    plasma membrane GO:0005886 ISO
    plasma membrane GO:0005886 NAS
    endosome membrane GO:0010008 ISO
    midbody GO:0030496 ISO
    midbody GO:0030496 ISS
    midbody GO:0030496 NAS
    late endosome membrane GO:0031902 IEA
    perinuclear region of cytoplasm GO:0048471 IDA
    perinuclear region of cytoplasm GO:0048471 ISO
    Flemming body GO:0090543 IEA
    Flemming body GO:0090543 ISO
    ATPase complex GO:1904949 NAS
 Experiment description of studies that identified Vps4a in sEVs
1
Experiment ID 907
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 908
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Vps4a
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Fancd2  
Affinity Capture-MS Mus musculus
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