Gene description for Ambp
Gene name alpha 1 microglobulin/bikunin
Gene symbol Ambp
Other names/aliases AI194774
ASPI
HI-30
Intin4
Itil
UTI
Species Mus musculus
 Database cross references - Ambp
ExoCarta ExoCarta_11699
Vesiclepedia VP_11699
Entrez Gene 11699
UniProt Q07456  
 Ambp identified in sEVs derived from the following tissue/cell type
Faeces 33431899    
Faeces 33431899    
 Gene ontology annotations for Ambp
Molecular Function
    serine-type endopeptidase inhibitor activity GO:0004867 IBA
    serine-type endopeptidase inhibitor activity GO:0004867 IEA
    serine-type endopeptidase inhibitor activity GO:0004867 ISO
    serine-type endopeptidase inhibitor activity GO:0004867 ISS
    antioxidant activity GO:0016209 ISO
    antioxidant activity GO:0016209 ISS
    oxidoreductase activity GO:0016491 IEA
    oxidoreductase activity, acting on NAD(P)H, heme protein as acceptor GO:0016653 ISO
    oxidoreductase activity, acting on NAD(P)H, heme protein as acceptor GO:0016653 ISS
    IgA binding GO:0019862 ISO
    IgA binding GO:0019862 ISS
    heme binding GO:0020037 ISO
    heme binding GO:0020037 ISS
    carbohydrate binding GO:0030246 ISO
    chondroitin sulfate binding GO:0035374 ISO
    chondroitin sulfate binding GO:0035374 ISS
    protein homodimerization activity GO:0042803 ISO
    protein homodimerization activity GO:0042803 ISS
    collagen fibril binding GO:0098633 ISO
    collagen fibril binding GO:0098633 ISS
Biological Process
    hemoglobin metabolic process GO:0020027 ISO
    hemoglobin metabolic process GO:0020027 ISS
    protein catabolic process GO:0030163 ISO
    cellular response to reactive oxygen species GO:0034614 ISO
    cellular response to reactive oxygen species GO:0034614 ISS
    protein maturation GO:0051604 ISO
    cellular oxidant detoxification GO:0098869 ISO
    cellular oxidant detoxification GO:0098869 ISS
    cytochrome c metabolic process GO:1903606 ISO
    cytochrome c metabolic process GO:1903606 ISS
Subcellular Localization
    extracellular region GO:0005576 IEA
    extracellular space GO:0005615 ISO
    extracellular space GO:0005615 ISS
    mitochondrial inner membrane GO:0005743 IEA
    endoplasmic reticulum GO:0005783 IEA
    cytosol GO:0005829 IEA
    plasma membrane GO:0005886 IBA
    plasma membrane GO:0005886 ISO
    plasma membrane GO:0005886 ISS
    cell surface GO:0009986 IBA
    cell surface GO:0009986 ISO
    nuclear membrane GO:0031965 IEA
    collagen-containing extracellular matrix GO:0062023 HDA
    matrix side of mitochondrial inner membrane GO:0099617 ISO
 Experiment description of studies that identified Ambp in sEVs
1
Experiment ID 303
MISEV standards
EM
Biophysical techniques
Cd63|Cd81|Cd9
Enriched markers
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33431899    
Organism Mus musculus
Experiment description High-fat diet-induced upregulation of exosomal phosphatidylcholine contributes to insulin resistance
Authors "Kumar A, Sundaram K, Mu J, Dryden GW, Sriwastva MK, Lei C, Zhang L, Qiu X, Xu F, Yan J, Zhang X, Park JW, Merchant ML, Bohler HCL, Wang B, Zhang S, Qin C, Xu Z, Han X, McClain CJ, Teng Y, Zhang HG."
Journal name Nat Commun
Publication year 2021
Sample Faeces
Sample name Intestinal epithelial cells - C57BL/6
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient centrifugation
Flotation density -
Molecules identified in the study Protein
Lipid
miRNA
Methods used in the study Western blotting
Mass spectrometry
Immunofluorescence
miRNA array
HPLC
2
Experiment ID 304
MISEV standards
EM
Biophysical techniques
Cd63|Cd81|Cd9
Enriched markers
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33431899    
Organism Mus musculus
Experiment description High-fat diet-induced upregulation of exosomal phosphatidylcholine contributes to insulin resistance
Authors "Kumar A, Sundaram K, Mu J, Dryden GW, Sriwastva MK, Lei C, Zhang L, Qiu X, Xu F, Yan J, Zhang X, Park JW, Merchant ML, Bohler HCL, Wang B, Zhang S, Qin C, Xu Z, Han X, McClain CJ, Teng Y, Zhang HG."
Journal name Nat Commun
Publication year 2021
Sample Faeces
Sample name Intestinal epithelial cells - C57BL/6
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient centrifugation
Flotation density -
Molecules identified in the study Protein
Lipid
miRNA
Methods used in the study Western blotting
Mass spectrometry
Immunofluorescence
miRNA array
HPLC
 Protein-protein interactions for Ambp
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.



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