Gene description for Rraga
Gene name Ras-related GTP binding A
Gene symbol Rraga
Other names/aliases Raga
Species Rattus norvegicus
 Database cross references - Rraga
ExoCarta ExoCarta_117044
Vesiclepedia VP_117044
Entrez Gene 117044
UniProt Q63486  
 Rraga identified in sEVs derived from the following tissue/cell type
Reticulocytes 21828046    
Reticulocytes 21828046    
Reticulocytes 21828046    
Reticulocytes 21828046    
 Gene ontology annotations for Rraga
Molecular Function
    GTPase activity GO:0003924 IBA
    GTPase activity GO:0003924 IEA
    GTPase activity GO:0003924 ISO
    GTPase activity GO:0003924 ISS
    GTP binding GO:0005525 IBA
    GTP binding GO:0005525 IDA
    GTP binding GO:0005525 IEA
    GTP binding GO:0005525 ISO
    GTP binding GO:0005525 ISS
    ubiquitin protein ligase binding GO:0031625 IEA
    ubiquitin protein ligase binding GO:0031625 ISO
    protein homodimerization activity GO:0042803 IEA
    protein homodimerization activity GO:0042803 ISO
    protein homodimerization activity GO:0042803 ISS
    protein-membrane adaptor activity GO:0043495 IEA
    protein-membrane adaptor activity GO:0043495 ISO
    protein-membrane adaptor activity GO:0043495 ISS
    protein heterodimerization activity GO:0046982 IEA
    protein heterodimerization activity GO:0046982 ISO
    protein heterodimerization activity GO:0046982 ISS
    phosphoprotein binding GO:0051219 IEA
    phosphoprotein binding GO:0051219 ISO
    phosphoprotein binding GO:0051219 ISS
    membrane scission GTPase motor activity GO:1990606 IEA
Biological Process
    apoptotic process GO:0006915 IEA
    protein localization GO:0008104 ISO
    protein localization GO:0008104 ISS
    biological_process GO:0008150 ND
    cellular response to starvation GO:0009267 IBA
    cellular response to starvation GO:0009267 ISO
    negative regulation of autophagy GO:0010507 IBA
    negative regulation of autophagy GO:0010507 IEA
    negative regulation of autophagy GO:0010507 ISO
    negative regulation of autophagy GO:0010507 ISS
    cellular response to nutrient levels GO:0031669 ISO
    positive regulation of TOR signaling GO:0032008 ISO
    tumor necrosis factor-mediated signaling pathway GO:0033209 IEA
    tumor necrosis factor-mediated signaling pathway GO:0033209 ISO
    cellular response to amino acid starvation GO:0034198 IEA
    cellular response to amino acid starvation GO:0034198 ISO
    cellular response to amino acid starvation GO:0034198 ISS
    intracellular signal transduction GO:0035556 IEA
    intracellular signal transduction GO:0035556 ISO
    glucose homeostasis GO:0042593 IEA
    glucose homeostasis GO:0042593 ISO
    response to amino acid GO:0043200 ISS
    protein localization to lysosome GO:0061462 IEA
    protein localization to lysosome GO:0061462 ISO
    cellular response to amino acid stimulus GO:0071230 IEA
    cellular response to amino acid stimulus GO:0071230 ISO
    cellular response to amino acid stimulus GO:0071230 ISS
    protein localization to membrane GO:0072657 IEA
    protein localization to membrane GO:0072657 ISO
    protein localization to membrane GO:0072657 ISS
    regulation of TORC1 signaling GO:1903432 ISS
    positive regulation of TORC1 signaling GO:1904263 IBA
    positive regulation of TORC1 signaling GO:1904263 IEA
    positive regulation of TORC1 signaling GO:1904263 ISO
    positive regulation of TORC1 signaling GO:1904263 ISS
Subcellular Localization
    nucleus GO:0005634 IBA
    nucleus GO:0005634 ISO
    nucleus GO:0005634 ISS
    nucleoplasm GO:0005654 IEA
    nucleoplasm GO:0005654 ISO
    cytoplasm GO:0005737 ISO
    cytoplasm GO:0005737 ISS
    lysosome GO:0005764 IBA
    lysosome GO:0005764 ISO
    lysosome GO:0005764 ISS
    lysosomal membrane GO:0005765 IEA
    lysosomal membrane GO:0005765 ISO
    lysosomal membrane GO:0005765 ISS
    GATOR1 complex GO:1990130 IEA
    GATOR1 complex GO:1990130 ISO
    GATOR1 complex GO:1990130 ISS
    Gtr1-Gtr2 GTPase complex GO:1990131 IBA
    FNIP-folliculin RagC/D GAP GO:1990877 IEA
    FNIP-folliculin RagC/D GAP GO:1990877 ISO
 Experiment description of studies that identified Rraga in sEVs
1
Experiment ID 95
MISEV standards
EM
Biophysical techniques
TSG101|HSP90|HSC70|MHCI
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors "Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E."
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D7 Rep 1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.25 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
2
Experiment ID 96
MISEV standards
EM
Biophysical techniques
TSG101|HSP90|HSC70|MHCI
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors "Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E."
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D2 Rep 2
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.16-1.21 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
3
Experiment ID 97
MISEV standards
EM
Biophysical techniques
TSG101|HSP90|HSC70|MHCI
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors "Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E."
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D4 Rep 2
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.16-1.21 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
4
Experiment ID 98
MISEV standards
EM
Biophysical techniques
TSG101|HSP90|HSC70|MHCI
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors "Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E."
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D7 Rep 2
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.25 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
 Protein-protein interactions for Rraga
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.



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