Gene description for Ptpn12
Gene name protein tyrosine phosphatase, non-receptor type 12
Gene symbol Ptpn12
Other names/aliases Ptpg1
RKPTP
Species Rattus norvegicus
 Database cross references - Ptpn12
ExoCarta ExoCarta_117255
Vesiclepedia VP_117255
Entrez Gene 117255
 Ptpn12 identified in sEVs derived from the following tissue/cell type
Reticulocytes 21828046    
 Gene ontology annotations for Ptpn12
Molecular Function
    phosphoprotein phosphatase activity GO:0004721 ISO
    protein tyrosine phosphatase activity GO:0004725 IBA
    protein tyrosine phosphatase activity GO:0004725 IDA
    protein tyrosine phosphatase activity GO:0004725 ISO
    non-membrane spanning protein tyrosine phosphatase activity GO:0004726 IEA
    SH3 domain binding GO:0017124 ISO
    protein tyrosine phosphatase activity, metal-dependent GO:0030946 IEA
    histone H2AXY142 phosphatase activity GO:0140793 IEA
Biological Process
    chromatin remodeling GO:0006338 IEA
    protein dephosphorylation GO:0006470 ISO
    protein dephosphorylation GO:0006470 ISO
    peptidyl-tyrosine dephosphorylation GO:0035335 ISO
    regulation of epidermal growth factor receptor signaling pathway GO:0042058 IBA
    regulation of epidermal growth factor receptor signaling pathway GO:0042058 ISO
    tissue regeneration GO:0042246 IEP
    cellular response to epidermal growth factor stimulus GO:0071364 ISO
Subcellular Localization
    podosome GO:0002102 ISO
    nucleus GO:0005634 IBA
    cytoplasm GO:0005737 IBA
    cytosol GO:0005829 ISO
 Experiment description of studies that identified Ptpn12 in sEVs
1
Experiment ID 96
MISEV standards
EM
Biophysical techniques
TSG101|HSP90|HSC70|MHCI
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors "Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E."
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D2 Rep 2
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.16-1.21 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
 Protein-protein interactions for Ptpn12
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.
 Pathways in which Ptpn12 is involved
No pathways found





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