Gene description for Prpsap2
Gene name phosphoribosyl pyrophosphate synthetase-associated protein 2
Gene symbol Prpsap2
Other names/aliases Pap41
Species Rattus norvegicus
 Database cross references - Prpsap2
ExoCarta ExoCarta_117272
Vesiclepedia VP_117272
Entrez Gene 117272
UniProt O08618  
 Prpsap2 identified in sEVs derived from the following tissue/cell type
Reticulocytes 21828046    
 Gene ontology annotations for Prpsap2
Molecular Function
    magnesium ion binding GO:0000287 IEA
    enzyme regulator activity GO:0030234 IBA
    identical protein binding GO:0042802 ISO
Biological Process
    5-phosphoribose 1-diphosphate biosynthetic process GO:0006015 IBA
    purine nucleotide biosynthetic process GO:0006164 IBA
    bone development GO:0060348 ISO
Subcellular Localization
    ribose phosphate diphosphokinase complex GO:0002189 IDA
    cytoplasm GO:0005737 IBA
    protein-containing complex GO:0032991 IDA
 Experiment description of studies that identified Prpsap2 in sEVs
1
Experiment ID 95
MISEV standards
EM
Biophysical techniques
TSG101|HSP90|HSC70|MHCI
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors "Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E."
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D7 Rep 1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.25 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
 Protein-protein interactions for Prpsap2
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.
 Pathways in which Prpsap2 is involved
No pathways found





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