Gene description for Ank
Gene name progressive ankylosis
Gene symbol Ank
Other names/aliases Ankh
D15Ertd221e
ank
mKIAA1581
Species Mus musculus
 Database cross references - Ank
ExoCarta ExoCarta_11732
Vesiclepedia VP_11732
Entrez Gene 11732
UniProt Q9JHZ2  
 Ank identified in sEVs derived from the following tissue/cell type
Colon cancer cells 37309723    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Ank
Molecular Function
    phosphate transmembrane transporter activity GO:0005315 IBA
    phosphate transmembrane transporter activity GO:0005315 IEA
    phosphate transmembrane transporter activity GO:0005315 ISO
    ATP transmembrane transporter activity GO:0005347 IEA
    ATP transmembrane transporter activity GO:0005347 ISO
    inorganic diphosphate transmembrane transporter activity GO:0030504 IBA
    inorganic diphosphate transmembrane transporter activity GO:0030504 IDA
    inorganic diphosphate transmembrane transporter activity GO:0030504 ISO
Biological Process
    gene expression GO:0010467 IDA
    gene expression GO:0010467 IMP
    bone mineralization GO:0030282 IDA
    bone mineralization GO:0030282 IMP
    regulation of bone mineralization GO:0030500 IMP
    regulation of bone mineralization GO:0030500 IMP
    inorganic diphosphate transport GO:0030505 IMP
    phosphate ion transmembrane transport GO:0035435 IEA
    muscle cell cellular homeostasis GO:0046716 IMP
    phosphate ion homeostasis GO:0055062 IMP
    calcium ion homeostasis GO:0055074 IMP
    diphosphate metabolic process GO:0071344 IDA
    diphosphate metabolic process GO:0071344 IMP
    cementum mineralization GO:0071529 IDA
    cementum mineralization GO:0071529 IMP
    inhibition of non-skeletal tissue mineralization GO:0140928 IDA
    inhibition of non-skeletal tissue mineralization GO:0140928 IMP
    response to sodium phosphate GO:1904383 IMP
    ATP export GO:1904669 IEA
    ATP export GO:1904669 ISO
Subcellular Localization
    extracellular region GO:0005576 IMP
    plasma membrane GO:0005886 IBA
    plasma membrane GO:0005886 IDA
    plasma membrane GO:0005886 ISO
    membrane GO:0016020 IDA
    membrane GO:0016020 ISO
 Experiment description of studies that identified Ank in sEVs
1
Experiment ID 907
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 908
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Ank
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Usp7  
Affinity Capture-MS Mus musculus
2 Tmem173  
Proximity Label-MS Mus musculus
3 Sstr3  
Proximity Label-MS Mus musculus
View the network image/svg+xml
 Pathways in which Ank is involved
PathwayEvidenceSource
Miscellaneous transport and binding events IEA Reactome
Transport of small molecules IEA Reactome





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