Gene description for Plscr1
Gene name phospholipid scramblase 1
Gene symbol Plscr1
Other names/aliases -
Species Rattus norvegicus
 Database cross references - Plscr1
ExoCarta ExoCarta_117540
Vesiclepedia VP_117540
Entrez Gene 117540
UniProt P58195  
 Plscr1 identified in sEVs derived from the following tissue/cell type
Reticulocytes 21828046    
Reticulocytes 21828046    
Reticulocytes 21828046    
 Gene ontology annotations for Plscr1
Molecular Function
    magnesium ion binding GO:0000287 ISO
    magnesium ion binding GO:0000287 ISS
    DNA-binding transcription activator activity, RNA polymerase II-specific GO:0001228 ISO
    DNA-binding transcription activator activity, RNA polymerase II-specific GO:0001228 ISS
    virus receptor activity GO:0001618 ISO
    DNA binding GO:0003677 IEA
    nuclease activity GO:0004518 ISO
    nuclease activity GO:0004518 ISS
    epidermal growth factor receptor binding GO:0005154 ISO
    epidermal growth factor receptor binding GO:0005154 ISS
    calcium ion binding GO:0005509 ISO
    calcium ion binding GO:0005509 ISS
    zinc ion binding GO:0008270 ISO
    zinc ion binding GO:0008270 ISS
    SH3 domain binding GO:0017124 ISO
    SH3 domain binding GO:0017124 ISS
    phospholipid scramblase activity GO:0017128 IBA
    phospholipid scramblase activity GO:0017128 IEA
    phospholipid scramblase activity GO:0017128 ISO
    phospholipid scramblase activity GO:0017128 ISS
    enzyme binding GO:0019899 IPI
    enzyme binding GO:0019899 ISO
    lead ion binding GO:0032791 ISO
    lead ion binding GO:0032791 ISS
    CD4 receptor binding GO:0042609 ISO
    CD4 receptor binding GO:0042609 ISS
    mercury ion binding GO:0045340 ISO
    mercury ion binding GO:0045340 ISS
Biological Process
    phosphatidylserine biosynthetic process GO:0006659 ISO
    apoptotic process GO:0006915 ISO
    apoptotic process GO:0006915 ISS
    acute-phase response GO:0006953 ISO
    immune response GO:0006955 IMP
    response to lead ion GO:0010288 ISO
    positive regulation of gene expression GO:0010628 ISO
    phospholipid transport GO:0015914 TAS
    plasma membrane phospholipid scrambling GO:0017121 IEA
    plasma membrane phospholipid scrambling GO:0017121 IMP
    plasma membrane phospholipid scrambling GO:0017121 ISO
    myeloid cell differentiation GO:0030099 ISO
    regulation of mast cell activation GO:0033003 IMP
    response to interferon-alpha GO:0035455 ISO
    response to interferon-beta GO:0035456 ISO
    positive regulation of apoptotic process GO:0043065 ISO
    negative regulation of viral genome replication GO:0045071 ISO
    negative regulation of viral genome replication GO:0045071 ISS
    positive regulation of innate immune response GO:0045089 ISO
    positive regulation of innate immune response GO:0045089 ISS
    positive regulation of transcription by RNA polymerase II GO:0045944 ISO
    positive regulation of transcription by RNA polymerase II GO:0045944 ISS
    symbiont entry into host cell GO:0046718 IEA
    negative regulation of phagocytosis GO:0050765 ISO
    negative regulation of phagocytosis GO:0050765 ISS
    defense response to virus GO:0051607 ISO
    defense response to virus GO:0051607 ISS
    regulation of Fc receptor mediated stimulatory signaling pathway GO:0060368 IMP
    phosphatidylserine exposure on apoptotic cell surface GO:0070782 IBA
    phosphatidylserine exposure on apoptotic cell surface GO:0070782 ISO
    phosphatidylserine exposure on apoptotic cell surface GO:0070782 ISO
    phosphatidylserine exposure on apoptotic cell surface GO:0070782 ISS
    cellular response to lipopolysaccharide GO:0071222 ISO
    cellular response to cytokine stimulus GO:0071345 ISO
    intrinsic apoptotic signaling pathway GO:0097193 ISO
    positive regulation of intrinsic apoptotic signaling pathway in response to DNA damage GO:1902231 ISO
    positive regulation of chromosome separation GO:1905820 ISO
    positive regulation of chromosome separation GO:1905820 ISS
    positive regulation of DNA topoisomerase (ATP-hydrolyzing) activity GO:2000373 ISO
    positive regulation of DNA topoisomerase (ATP-hydrolyzing) activity GO:2000373 ISS
Subcellular Localization
    nucleus GO:0005634 ISO
    nucleus GO:0005634 ISS
    nucleoplasm GO:0005654 ISO
    nucleolus GO:0005730 ISO
    nucleolus GO:0005730 ISS
    cytoplasm GO:0005737 ISO
    Golgi apparatus GO:0005794 ISO
    Golgi apparatus GO:0005794 ISS
    cytosol GO:0005829 ISO
    cytosol GO:0005829 ISS
    plasma membrane GO:0005886 IBA
    plasma membrane GO:0005886 IDA
    plasma membrane GO:0005886 ISO
    plasma membrane GO:0005886 ISS
    membrane GO:0016020 IEA
    membrane raft GO:0045121 IDA
    membrane raft GO:0045121 ISO
    perinuclear region of cytoplasm GO:0048471 ISO
    perinuclear region of cytoplasm GO:0048471 ISS
    collagen-containing extracellular matrix GO:0062023 ISO
    collagen-containing extracellular matrix GO:0062023 ISS
 Experiment description of studies that identified Plscr1 in sEVs
1
Experiment ID 90
MISEV standards
✔
EM
Biophysical techniques
✔
HSC70|HSP90|TSG101|MHCI
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors "Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E"
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D2 Rep 1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.16-1.21 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
2
Experiment ID 94
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|HSP90|HSC70|MHCI
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors "Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E."
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D4 Rep 1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.16-1.21 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
3
Experiment ID 97
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|HSP90|HSC70|MHCI
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors "Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E."
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D4 Rep 2
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.16-1.21 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
 Protein-protein interactions for Plscr1
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.
 Pathways in which Plscr1 is involved
No pathways found





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