Gene description for Plscr1
Gene name phospholipid scramblase 1
Gene symbol Plscr1
Other names/aliases -
Species Rattus norvegicus
 Database cross references - Plscr1
ExoCarta ExoCarta_117540
Entrez Gene 117540
UniProt P58195  
 Plscr1 identified in exosomes derived from the following tissue/cell type
Reticulocytes 21828046    
Reticulocytes 21828046    
Reticulocytes 21828046    
 Gene ontology annotations for Plscr1
Molecular Function
    SH3 domain binding GO:0017124 ISS
    phospholipid scramblase activity GO:0017128 ISS
    DNA binding GO:0003677 IEA
    enzyme binding GO:0019899 ISO
    epidermal growth factor receptor binding GO:0005154 ISS
    calcium ion binding GO:0005509 ISS
    RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in positive regulation of transcription GO:0001077 ISS
    CD4 receptor binding GO:0042609 ISS
Biological Process
    response to interferon-alpha GO:0035455 ISO
    positive regulation of innate immune response GO:0045089 ISS
    negative regulation of protein binding GO:0032091 ISO
    acute-phase response GO:0006953 IEA
    negative regulation of viral genome replication GO:0045071 ISS
    immune response GO:0006955 IMP
    phosphatidylserine exposure on apoptotic cell surface GO:0070782 ISO
    regulation of Fc receptor mediated stimulatory signaling pathway GO:0060368 IMP
    positive regulation of intrinsic apoptotic signaling pathway in response to DNA damage GO:1902231 ISO
    response to interferon-beta GO:0035456 ISO
    positive regulation of apoptotic process GO:0043065 ISO
    positive regulation of transcription from RNA polymerase II promoter GO:0045944 ISS
    phospholipid scrambling GO:0017121 ISO
    apoptotic process GO:0006915 ISS
    phospholipid transport GO:0015914 TAS
    defense response to virus GO:0051607 ISS
    positive regulation of gene expression GO:0010628 ISO
    phosphatidylserine biosynthetic process GO:0006659 ISO
    positive regulation of DNA topoisomerase (ATP-hydrolyzing) activity GO:2000373 ISS
    regulation of mast cell activation GO:0033003 IMP
    intrinsic apoptotic signaling pathway GO:0097193 ISO
    myeloid cell differentiation GO:0030099 ISO
    cellular response to lipopolysaccharide GO:0071222 IEA
    cellular response to cytokine stimulus GO:0071345 IEA
Subcellular Localization
    extracellular matrix GO:0031012 ISS
    cytosol GO:0005829 ISS
    extracellular exosome GO:0070062 ISO
    integral component of plasma membrane GO:0005887 ISS
    plasma membrane GO:0005886 ISO
    membrane raft GO:0045121 ISO
    nucleolus GO:0005730 ISS
    Golgi apparatus GO:0005794 ISS
    nucleus GO:0005634 ISO
    membrane GO:0016020 ISO
 Experiment description of studies that identified Plscr1 in exosomes
1
Experiment ID 90
ISEV standards
EM
EV Biophysical techniques
HSC70|HSP90|TSG101
EV Cytosolic markers
MHCI
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D2 Rep 1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.16-1.21 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
2
Experiment ID 94
ISEV standards
EM
EV Biophysical techniques
TSG101|HSP90|HSC70
EV Cytosolic markers
MHCI
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E.
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D4 Rep 1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.16-1.21 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
3
Experiment ID 97
ISEV standards
EM
EV Biophysical techniques
TSG101|HSP90|HSC70
EV Cytosolic markers
MHCI
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E.
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D4 Rep 2
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.16-1.21 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
 Protein-protein interactions for Plscr1
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.
 Pathways in which Plscr1 is involved
No pathways found





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