Gene description for Kif5b
Gene name kinesin family member 5B
Gene symbol Kif5b
Other names/aliases Khc
Species Rattus norvegicus
 Database cross references - Kif5b
ExoCarta ExoCarta_117550
Vesiclepedia VP_117550
Entrez Gene 117550
UniProt Q2PQA9  
 Kif5b identified in sEVs derived from the following tissue/cell type
Reticulocytes 21828046    
Reticulocytes 21828046    
Reticulocytes 21828046    
 Gene ontology annotations for Kif5b
Molecular Function
    microtubule motor activity GO:0003777 IDA
    microtubule motor activity GO:0003777 IEA
    microtubule motor activity GO:0003777 ISO
    protein binding GO:0005515 IPI
    ATP binding GO:0005524 IEA
    microtubule binding GO:0008017 IBA
    microtubule binding GO:0008017 IDA
    microtubule binding GO:0008017 IEA
    microtubule binding GO:0008017 ISO
    JUN kinase binding GO:0008432 IPI
    plus-end-directed microtubule motor activity GO:0008574 IBA
    ATP hydrolysis activity GO:0016887 IBA
    identical protein binding GO:0042802 ISO
    protein-containing complex binding GO:0044877 IEA
    protein-containing complex binding GO:0044877 ISO
    microtubule lateral binding GO:0099609 IMP
Biological Process
    cytoplasm organization GO:0007028 IEA
    cytoplasm organization GO:0007028 ISO
    axon guidance GO:0007411 IBA
    hippocampus development GO:0021766 IEP
    positive regulation of vesicle fusion GO:0031340 IMP
    positive regulation of synaptic transmission, GABAergic GO:0032230 IEA
    positive regulation of synaptic transmission, GABAergic GO:0032230 ISO
    lysosome localization GO:0032418 ISO
    lysosome localization GO:0032418 ISS
    stress granule disassembly GO:0035617 IEA
    stress granule disassembly GO:0035617 ISO
    positive regulation of insulin secretion involved in cellular response to glucose stimulus GO:0035774 IMP
    natural killer cell mediated cytotoxicity GO:0042267 ISO
    natural killer cell mediated cytotoxicity GO:0042267 ISS
    regulation of membrane potential GO:0042391 ISO
    positive regulation of potassium ion transport GO:0043268 ISO
    vesicle transport along microtubule GO:0047496 IDA
    vesicle transport along microtubule GO:0047496 ISO
    vesicle transport along microtubule GO:0047496 ISO
    mitochondrion transport along microtubule GO:0047497 IEA
    mitochondrion transport along microtubule GO:0047497 ISO
    synaptic vesicle transport GO:0048489 IBA
    centrosome localization GO:0051642 ISO
    centrosome localization GO:0051642 ISS
    cellular response to type II interferon GO:0071346 IEA
    cellular response to type II interferon GO:0071346 ISO
    plus-end-directed vesicle transport along microtubule GO:0072383 IEA
    plus-end-directed vesicle transport along microtubule GO:0072383 ISO
    positive regulation of intracellular protein transport GO:0090316 IMP
    anterograde dendritic transport of neurotransmitter receptor complex GO:0098971 IBA
    regulation of modification of synapse structure, modulating synaptic transmission GO:0098987 IEA
    regulation of modification of synapse structure, modulating synaptic transmission GO:0098987 ISO
    anterograde axonal protein transport GO:0099641 IMP
    mitocytosis GO:0160040 IEA
    mitocytosis GO:0160040 ISO
    positive regulation of protein localization to plasma membrane GO:1903078 ISO
    positive regulation of voltage-gated sodium channel activity GO:1905152 IMP
    retrograde neuronal dense core vesicle transport GO:1990049 IGI
Subcellular Localization
    cytoplasm GO:0005737 IBA
    cytoplasm GO:0005737 ISO
    mitochondrion GO:0005739 IEA
    mitochondrion GO:0005739 ISO
    cytosol GO:0005829 ISO
    kinesin complex GO:0005871 IBA
    microtubule GO:0005874 IBA
    microtubule GO:0005874 IEA
    microtubule cytoskeleton GO:0015630 IDA
    endocytic vesicle GO:0030139 ISO
    vesicle GO:0031982 ISO
    vesicle GO:0031982 ISS
    dendrite cytoplasm GO:0032839 IEA
    centriolar satellite GO:0034451 ISO
    ciliary rootlet GO:0035253 IEA
    ciliary rootlet GO:0035253 ISO
    neuron projection GO:0043005 IEA
    neuron projection GO:0043005 ISO
    axonal growth cone GO:0044295 IDA
    phagocytic vesicle GO:0045335 IEA
    phagocytic vesicle GO:0045335 ISO
    perinuclear region of cytoplasm GO:0048471 IDA
    postsynaptic cytosol GO:0099524 IDA
    postsynaptic cytosol GO:0099524 IEA
    postsynaptic cytosol GO:0099524 ISO
    cytolytic granule membrane GO:0101004 IEA
    axon cytoplasm GO:1904115 IEA
 Experiment description of studies that identified Kif5b in sEVs
1
Experiment ID 95
MISEV standards
EM
Biophysical techniques
TSG101|HSP90|HSC70|MHCI
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors "Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E."
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D7 Rep 1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.25 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
2
Experiment ID 97
MISEV standards
EM
Biophysical techniques
TSG101|HSP90|HSC70|MHCI
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors "Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E."
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D4 Rep 2
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.16-1.21 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
3
Experiment ID 98
MISEV standards
EM
Biophysical techniques
TSG101|HSP90|HSC70|MHCI
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors "Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E."
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D7 Rep 2
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.25 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
 Protein-protein interactions for Kif5b
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 STXBP5L  
Affinity Capture-MS Homo sapiens
2 Itm2b 290364
Affinity Capture-MS Rattus norvegicus
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