Gene description for Kif5b
Gene name kinesin family member 5B
Gene symbol Kif5b
Other names/aliases Khc
Species Rattus norvegicus
 Database cross references - Kif5b
ExoCarta ExoCarta_117550
Entrez Gene 117550
UniProt Q2PQA9  
 Kif5b identified in exosomes derived from the following tissue/cell type
Reticulocytes 21828046    
Reticulocytes 21828046    
Reticulocytes 21828046    
 Gene ontology annotations for Kif5b
Molecular Function
    protein binding GO:0005515 IPI
    ATP binding GO:0005524 IEA
    ATP-dependent microtubule motor activity, plus-end-directed GO:0008574 IBA
    microtubule motor activity GO:0003777 IDA
    microtubule binding GO:0008017 IDA
Biological Process
    positive regulation of synaptic transmission, GABAergic GO:0032230 ISO
    metabolic process GO:0008152 IEA
    cytoplasm organization GO:0007028 ISO
    positive regulation of potassium ion transport GO:0043268 ISO
    positive regulation of establishment of protein localization to plasma membrane GO:0090004 ISO
    regulation of membrane potential GO:0042391 ISO
    stress granule disassembly GO:0035617 ISO
    protein localization GO:0008104 IBA
    vesicle transport along microtubule GO:0047496 ISO
    plus-end-directed vesicle transport along microtubule GO:0072383 ISO
    axon guidance GO:0007411 IBA
Subcellular Localization
    perinuclear region of cytoplasm GO:0048471 IDA
    endocytic vesicle GO:0030139 ISO
    membrane GO:0016020 ISO
    cytoplasm GO:0005737 ISO
    microtubule GO:0005874 IEA
    kinesin complex GO:0005871 IBA
    neuron projection GO:0043005 ISO
    microtubule organizing center GO:0005815 ISO
    membrane-bounded organelle GO:0043227 ISO
    ciliary rootlet GO:0035253 ISO
    vesicle GO:0031982 ISS
    cell GO:0005623 ISO
    intracellular GO:0005622 ISO
 Experiment description of studies that identified Kif5b in exosomes
1
Experiment ID 95
ISEV standards
EM
EV Biophysical techniques
TSG101|HSP90|HSC70
EV Cytosolic markers
MHCI
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E.
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D7 Rep 1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.25 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
2
Experiment ID 97
ISEV standards
EM
EV Biophysical techniques
TSG101|HSP90|HSC70
EV Cytosolic markers
MHCI
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E.
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D4 Rep 2
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.16-1.21 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
3
Experiment ID 98
ISEV standards
EM
EV Biophysical techniques
TSG101|HSP90|HSC70
EV Cytosolic markers
MHCI
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E.
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D7 Rep 2
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.25 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
 Protein-protein interactions for Kif5b
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.
 Pathways in which Kif5b is involved
PathwayEvidenceSource
Insulin processing IEA Reactome





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