Gene description for Uba3
Gene name ubiquitin-like modifier activating enzyme 3
Gene symbol Uba3
Other names/aliases Ube1c
Species Rattus norvegicus
 Database cross references - Uba3
ExoCarta ExoCarta_117553
Entrez Gene 117553
UniProt Q99MI7  
 Uba3 identified in exosomes derived from the following tissue/cell type
Reticulocytes 21828046    
 Gene ontology annotations for Uba3
Molecular Function
    acid-amino acid ligase activity GO:0016881 IEA
    NEDD8 activating enzyme activity GO:0019781 ISO
    small protein activating enzyme activity GO:0008641 IEA
    ligand-dependent nuclear receptor binding GO:0016922 IPI
    protein heterodimerization activity GO:0046982 ISO
    ATP binding GO:0005524 IEA
Biological Process
    endomitotic cell cycle GO:0007113 ISO
    mitotic cell cycle GO:0000278 ISO
    negative regulation of transcription, DNA-templated GO:0045892 IDA
    regulation of cell cycle GO:0051726 ISO
    protein neddylation GO:0045116 TAS
Subcellular Localization
    nucleus GO:0005634 ISO
    cytosol GO:0005829 IBA
 Experiment description of studies that identified Uba3 in exosomes
1
Experiment ID 95
ISEV standards
EM
EV Biophysical techniques
TSG101|HSP90|HSC70
EV Cytosolic markers
MHCI
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E.
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D7 Rep 1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.25 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
 Protein-protein interactions for Uba3
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.
 Pathways in which Uba3 is involved
No pathways found





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