Gene description for Uba3
Gene name ubiquitin-like modifier activating enzyme 3
Gene symbol Uba3
Other names/aliases Ube1c
Species Rattus norvegicus
 Database cross references - Uba3
ExoCarta ExoCarta_117553
Vesiclepedia VP_117553
Entrez Gene 117553
UniProt Q99MI7  
 Uba3 identified in sEVs derived from the following tissue/cell type
Reticulocytes 21828046    
 Gene ontology annotations for Uba3
Molecular Function
    ATP binding GO:0005524 IEA
    nuclear receptor binding GO:0016922 IPI
    NEDD8 activating enzyme activity GO:0019781 IDA
    NEDD8 activating enzyme activity GO:0019781 IEA
    NEDD8 activating enzyme activity GO:0019781 IMP
    NEDD8 activating enzyme activity GO:0019781 ISO
    NEDD8 activating enzyme activity GO:0019781 ISO
    identical protein binding GO:0042802 IEA
    identical protein binding GO:0042802 ISO
    protein-containing complex binding GO:0044877 IMP
    protein heterodimerization activity GO:0046982 IEA
    protein heterodimerization activity GO:0046982 ISO
Biological Process
    mitotic cell cycle GO:0000278 ISO
    endomitotic cell cycle GO:0007113 IEA
    endomitotic cell cycle GO:0007113 ISO
    protein neddylation GO:0045116 IBA
    protein neddylation GO:0045116 IEA
    protein neddylation GO:0045116 ISO
    protein neddylation GO:0045116 TAS
    negative regulation of DNA-templated transcription GO:0045892 IDA
    regulation of cell cycle GO:0051726 IEA
    regulation of cell cycle GO:0051726 ISO
Subcellular Localization
    nucleus GO:0005634 IBA
    nucleus GO:0005634 IEA
    nucleus GO:0005634 ISO
    cytoplasm GO:0005737 IBA
    protein-containing complex GO:0032991 IEA
    protein-containing complex GO:0032991 ISO
 Experiment description of studies that identified Uba3 in sEVs
1
Experiment ID 95
MISEV standards
EM
Biophysical techniques
TSG101|HSP90|HSC70|MHCI
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors "Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E."
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D7 Rep 1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.25 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
 Protein-protein interactions for Uba3
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Esr1  
Two-hybrid Rattus norvegicus
Reconstituted Complex Rattus norvegicus
View the network image/svg+xml



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