Gene description for Arf4
Gene name ADP-ribosylation factor 4
Gene symbol Arf4
Other names/aliases AA407803
Species Mus musculus
 Database cross references - Arf4
ExoCarta ExoCarta_11843
Vesiclepedia VP_11843
Entrez Gene 11843
UniProt P61750  
 Arf4 identified in sEVs derived from the following tissue/cell type
Colon cancer cells 37309723    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Arf4
Molecular Function
    GTPase activity GO:0003924 IEA
    epidermal growth factor receptor binding GO:0005154 IEA
    epidermal growth factor receptor binding GO:0005154 ISO
    protein binding GO:0005515 IPI
    GTP binding GO:0005525 IBA
    GTP binding GO:0005525 IEA
    GTP binding GO:0005525 ISO
    NAD+-protein-arginine ADP-ribosyltransferase activity GO:0106274 IEA
    NAD+-protein-arginine ADP-ribosyltransferase activity GO:0106274 ISO
    phospholipase D activator activity GO:1990583 IEA
    phospholipase D activator activity GO:1990583 ISO
Biological Process
    intracellular protein transport GO:0006886 IBA
    retrograde vesicle-mediated transport, Golgi to endoplasmic reticulum GO:0006890 IEA
    retrograde vesicle-mediated transport, Golgi to endoplasmic reticulum GO:0006890 ISO
    epidermal growth factor receptor signaling pathway GO:0007173 ISO
    learning GO:0007612 IMP
    protein transport GO:0015031 IEA
    vesicle-mediated transport GO:0016192 IBA
    cell migration GO:0016477 IEA
    cell migration GO:0016477 ISO
    negative regulation of apoptotic process GO:0043066 IEA
    negative regulation of apoptotic process GO:0043066 ISO
    apical protein localization GO:0045176 IMP
    establishment or maintenance of epithelial cell apical/basal polarity GO:0045197 IMP
    positive regulation of transcription by RNA polymerase II GO:0045944 IEA
    positive regulation of transcription by RNA polymerase II GO:0045944 ISO
    regulation of synapse organization GO:0050807 IDA
    regulation of synapse organization GO:0050807 IMP
    dendritic spine development GO:0060996 IMP
    dendritic spine development GO:0060996 ISO
    protein localization to cilium GO:0061512 IMP
    regulation of postsynapse organization GO:0099175 IDA
    regulation of postsynapse organization GO:0099175 IMP
    regulation of cilium assembly GO:1902017 ISO
    regulation of cilium assembly GO:1902017 ISS
    regulation of reactive oxygen species metabolic process GO:2000377 IEA
    regulation of reactive oxygen species metabolic process GO:2000377 ISO
Subcellular Localization
    Golgi membrane GO:0000139 ISO
    Golgi membrane GO:0000139 ISS
    cytoplasm GO:0005737 IBA
    cytoplasm GO:0005737 IDA
    Golgi apparatus GO:0005794 IDA
    cytosol GO:0005829 IEA
    cytosol GO:0005829 ISO
    plasma membrane GO:0005886 IBA
    plasma membrane GO:0005886 IDA
    ruffle membrane GO:0032587 IEA
    ruffle membrane GO:0032587 ISO
    dendritic spine GO:0043197 ISO
    glutamatergic synapse GO:0098978 IDA
    glutamatergic synapse GO:0098978 IMP
 Experiment description of studies that identified Arf4 in sEVs
1
Experiment ID 907
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 908
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Arf4
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Nphs2  
Affinity Capture-MS Mus musculus
2 Atxn1  
Affinity Capture-MS Mus musculus
3 Eed  
Affinity Capture-MS Mus musculus
4 Mapt  
Affinity Capture-MS Mus musculus
5 Unk  
Affinity Capture-RNA Mus musculus
6 Fancd2  
Affinity Capture-MS Mus musculus
7 Kctd13  
Affinity Capture-MS Mus musculus
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