Experiment description of studies that identified Arf6 in exosomes

1 Experiment ID 89 ISEV standards ✔ EM EV Biophysical techniques ✘ EV Cytosolic markers ✘ EV Membrane markers ✘ EV Negative markers ✔ DLS EV Particle analysis Identified molecule protein Identification method Mass spectrometry PubMed ID 20424270     Organism Rattus norvegicus Experiment description Exosomes account for vesicle-mediated transcellular transport of activatable phospholipases and prostaglandins. Authors Subra C, Grand D, Laulagnier K, Stella A, Lambeau G, Paillasse M, De Medina P, Monsarrat B, Perret B, Silvente-Poirot S, Poirot M, Record M. Journal name JLR Publication year 2010 Sample Basophilic leukemia cells Sample name RBL-2H3 Isolation/purification methods Differential centrifugation Sucrose density gradient Flotation density - Molecules identified in the study Protein Lipids Methods used in the study Western blotting Gas chromatography Thin layer chromatography Mass spectrometry [Qstar XL] FACS

2 Experiment ID 210 ISEV standards ✔ EM EV Biophysical techniques ✘ EV Cytosolic markers ✔ CD81|FLOT1 EV Membrane markers ✘ EV Negative markers ✘ EV Particle analysis Identified molecule protein Identification method Mass spectrometry PubMed ID 23260141     Organism Mus musculus Experiment description Exosomes Mediate Stromal Mobilization of Autocrine Wnt-PCP Signaling in Breast Cancer Cell Migration. Authors Luga V, Zhang L, Viloria-Petit AM, Ogunjimi AA, Inanlou MR, Chiu E, Buchanan M, Hosein AN, Basik M, Wrana JL. Journal name Cell Publication year 2012 Sample Fibroblasts Sample name Normal-Fibroblasts (L cells) Isolation/purification methods Differential centrifugation Ultracentrifugation Flotation density - Molecules identified in the study Protein Methods used in the study Mass spectrometry

3 Experiment ID 15 ISEV standards ✔ EM EV Biophysical techniques ✘ EV Cytosolic markers ✔ CD63 EV Membrane markers ✘ EV Negative markers ✘ EV Particle analysis Identified molecule mrna Identification method Microarray PubMed ID 17486113     Organism Mus musculus Homo sapiens Experiment description Exosome-mediated transfer of mRNAs and microRNAs is a novel mechanism of genetic exchange between cells. Authors Valadi H, Ekstrom K, Bossios A, Sjostrand M, Lee JJ, Lotvall JO Journal name NCB Publication year 2007 Sample Mast cells Sample name MC9 Bone marrow-derived mast cells HMC-1 Isolation/purification methods Filtration Ultracentrifugation Sucrose density gradient Flotation density 1.11-1.21 g/mL Molecules identified in the study Protein mRNA miRNA Methods used in the study Mass spectrometry [MALDI TOF] Western blotting Microarray miRCURY LNA Array

4 Experiment ID 39 ISEV standards ✔ IEM EV Biophysical techniques ✔ TSG101|HSC70 EV Cytosolic markers ✔ FLOT1 EV Membrane markers ✘ EV Negative markers ✘ EV Particle analysis Identified molecule protein Identification method Mass spectrometry PubMed ID 15210972     Organism Mus musculus Experiment description Cells release prions in association with exosomes. Authors Fevrier B, Vilette D, Archer F, Loew D, Faigle W, Vidal M, Laude H, Raposo G Journal name PNAS Publication year 2004 Sample Mov neuroglial cells Sample name Mov neuroglial cell Isolation/purification methods Differential centrifugation Sucrose density gradient Flotation density 1.14 g/mL Molecules identified in the study Protein Methods used in the study Mass spectrometry [QSTAR] Western blotting Immunoelectron Microscopy

Protein-protein interactions for Arf6

Protein Interactor ExoCarta ID Identification method PubMed Species No interactions are found.

Pathways in which Arf6 is involved

No pathways found