Gene description for Atp8a1
Gene name ATPase, aminophospholipid transporter (APLT), class I, type 8A, member 1
Gene symbol Atp8a1
Other names/aliases AI481521
AI853962
APLT
AW743152
AW822227
Atp3a2
B230107D19Rik
ClassI
Species Mus musculus
 Database cross references - Atp8a1
ExoCarta ExoCarta_11980
Vesiclepedia VP_11980
Entrez Gene 11980
UniProt P70704  
 Atp8a1 identified in sEVs derived from the following tissue/cell type
Mast cells 17486113    
 Gene ontology annotations for Atp8a1
Molecular Function
    magnesium ion binding GO:0000287 IEA
    protein binding GO:0005515 IPI
    ATP binding GO:0005524 IEA
    ATP hydrolysis activity GO:0016887 IEA
    phosphatidylserine floppase activity GO:0090556 IEA
    ATPase-coupled intramembrane lipid transporter activity GO:0140326 IBA
    phosphatidylserine flippase activity GO:0140346 IMP
    phosphatidylserine flippase activity GO:0140346 ISO
    phosphatidylserine flippase activity GO:0140346 ISS
Biological Process
    learning GO:0007612 IMP
    positive regulation of cell migration GO:0030335 IMP
    phospholipid translocation GO:0045332 IBA
    synaptic vesicle endocytosis GO:0048488 EXP
    synaptic vesicle endocytosis GO:0048488 IDA
    synaptic vesicle endocytosis GO:0048488 IEP
    synaptic vesicle endocytosis GO:0048488 IMP
    positive regulation of phospholipid translocation GO:0061092 IDA
    aminophospholipid translocation GO:0140331 IMP
    aminophospholipid translocation GO:0140331 ISO
    aminophospholipid translocation GO:0140331 ISS
Subcellular Localization
    endoplasmic reticulum GO:0005783 IEA
    endoplasmic reticulum GO:0005783 ISO
    Golgi apparatus GO:0005794 IEA
    Golgi apparatus GO:0005794 ISO
    trans-Golgi network GO:0005802 IBA
    cytosol GO:0005829 IEA
    cytosol GO:0005829 ISO
    plasma membrane GO:0005886 IBA
    plasma membrane GO:0005886 IEA
    plasma membrane GO:0005886 ISO
    synaptic vesicle membrane GO:0030672 EXP
    synaptic vesicle membrane GO:0030672 IDA
    synaptic vesicle membrane GO:0030672 IEP
    synaptic vesicle membrane GO:0030672 IMP
    synaptic vesicle membrane GO:0030672 ISO
    organelle membrane GO:0031090 IDA
    cytoplasmic vesicle GO:0031410 IDA
    chromaffin granule membrane GO:0042584 IEA
    intracellular membrane-bounded organelle GO:0043231 ISO
    synapse GO:0045202 IDA
    glutamatergic synapse GO:0098978 EXP
    glutamatergic synapse GO:0098978 IDA
    glutamatergic synapse GO:0098978 IEP
    glutamatergic synapse GO:0098978 IMP
    phospholipid-translocating ATPase complex GO:1990531 ISO
    phospholipid-translocating ATPase complex GO:1990531 ISS
 Experiment description of studies that identified Atp8a1 in sEVs
1
Experiment ID 15
MISEV standards
EM
Biophysical techniques
CD63
Enriched markers
Negative markers
Particle analysis
Identified molecule mrna
Identification method Microarray
PubMed ID 17486113    
Organism Mus musculus
Homo sapiens
Experiment description Exosome-mediated transfer of mRNAs and microRNAs is a novel mechanism of genetic exchange between cells.
Authors "Valadi H, Ekstrom K, Bossios A, Sjostrand M, Lee JJ, Lotvall JO"
Journal name NCB
Publication year 2007
Sample Mast cells
Sample name MC9
Bone marrow-derived mast cells
HMC-1
Isolation/purification methods Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.11-1.21 g/mL
Molecules identified in the study Protein
mRNA
miRNA
Methods used in the study Mass spectrometry [MALDI TOF]
Western blotting
Microarray
miRCURY LNA Array
 Protein-protein interactions for Atp8a1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Kctd13  
Affinity Capture-MS Mus musculus
2 Tmem173  
Proximity Label-MS Mus musculus
View the network image/svg+xml



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