Gene description for Bcat1
Gene name branched chain aminotransferase 1, cytosolic
Gene symbol Bcat1
Other names/aliases BCATc
Eca39
Species Mus musculus
 Database cross references - Bcat1
ExoCarta ExoCarta_12035
Vesiclepedia VP_12035
Entrez Gene 12035
UniProt P24288  
 Bcat1 identified in sEVs derived from the following tissue/cell type
Colon cancer cells 37309723    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Bcat1
Molecular Function
    branched-chain-amino-acid transaminase activity GO:0004084 IBA
    branched-chain-amino-acid transaminase activity GO:0004084 IDA
    branched-chain-amino-acid transaminase activity GO:0004084 ISO
    identical protein binding GO:0042802 ISO
    L-leucine-2-oxoglutarate transaminase activity GO:0052654 ISS
    L-valine-2-oxoglutarate transaminase activity GO:0052655 ISS
    L-isoleucine-2-oxoglutarate transaminase activity GO:0052656 ISS
Biological Process
    lipid metabolic process GO:0006629 IEA
    amino acid biosynthetic process GO:0008652 IEA
    branched-chain amino acid biosynthetic process GO:0009082 IEA
    branched-chain amino acid catabolic process GO:0009083 IDA
    L-leucine biosynthetic process GO:0009098 IBA
    L-valine biosynthetic process GO:0009099 IBA
Subcellular Localization
    mitochondrion GO:0005739 HDA
    mitochondrion GO:0005739 IBA
    cytosol GO:0005829 IDA
 Experiment description of studies that identified Bcat1 in sEVs
1
Experiment ID 907
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 908
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Bcat1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Pus1  
Co-fractionation Mus musculus
2 Snf8 27681
Co-fractionation Mus musculus
3 Serpine2  
Co-fractionation Mus musculus
View the network image/svg+xml
 Pathways in which Bcat1 is involved
PathwayEvidenceSource
Branched-chain amino acid catabolism IEA Reactome
Metabolism IEA Reactome
Metabolism of amino acids and derivatives IEA Reactome





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