Gene description for Cspg4
Gene name chondroitin sulfate proteoglycan 4
Gene symbol Cspg4
Other names/aliases 4732461B14Rik
AN2
NG2
Species Mus musculus
 Database cross references - Cspg4
ExoCarta ExoCarta_121021
Vesiclepedia VP_121021
Entrez Gene 121021
UniProt Q8VHY0  
 Cspg4 identified in sEVs derived from the following tissue/cell type
Colon cancer cells 37309723    
Fibroblasts 23260141    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Cspg4
Molecular Function
    protein binding GO:0005515 IPI
    collagen binding GO:0005518 ISO
    coreceptor activity GO:0015026 IDA
    protein kinase binding GO:0019901 IEA
    protein kinase binding GO:0019901 ISO
Biological Process
    angiogenesis GO:0001525 IEA
    substrate-dependent cell migration GO:0006929 IGI
    glial cell migration GO:0008347 IDA
    negative regulation of neuron projection development GO:0010977 ISO
    neuron remodeling GO:0016322 ISO
    intracellular signal transduction GO:0035556 IEA
    intracellular signal transduction GO:0035556 ISO
    positive regulation of MAPK cascade GO:0043410 IMP
    platelet-derived growth factor receptor signaling pathway GO:0048008 IDA
    tissue remodeling GO:0048771 IEA
    positive regulation of peptidyl-tyrosine phosphorylation GO:0050731 IEA
    positive regulation of peptidyl-tyrosine phosphorylation GO:0050731 ISO
    ruffle assembly GO:0097178 IDA
Subcellular Localization
    ruffle GO:0001726 IDA
    nucleoplasm GO:0005654 IEA
    nucleoplasm GO:0005654 ISO
    plasma membrane GO:0005886 IDA
    plasma membrane GO:0005886 ISO
    cell surface GO:0009986 IEA
    apical plasma membrane GO:0016324 IEA
    lamellipodium membrane GO:0031258 IEA
    cell projection GO:0042995 IDA
    collagen-containing extracellular matrix GO:0062023 HDA
 Experiment description of studies that identified Cspg4 in sEVs
1
Experiment ID 907
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 210
MISEV standards
✔
EM
Biophysical techniques
✔
CD81|FLOT1
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23260141    
Organism Mus musculus
Experiment description Exosomes Mediate Stromal Mobilization of Autocrine Wnt-PCP Signaling in Breast Cancer Cell Migration.
Authors "Luga V, Zhang L, Viloria-Petit AM, Ogunjimi AA, Inanlou MR, Chiu E, Buchanan M, Hosein AN, Basik M, Wrana JL."
Journal name Cell
Publication year 2012
Sample Fibroblasts
Sample name Normal-Fibroblasts (L cells)
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
3
Experiment ID 908
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Cspg4
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Fancd2  
Affinity Capture-MS Mus musculus
2 Thrb  
Affinity Capture-MS Mus musculus
3 Grip1  
Co-fractionation Mus musculus
4 Notch3  
Co-fractionation Mus musculus
5 Gria2  
Co-fractionation Mus musculus
View the network image/svg+xml



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