Experiment description of studies that identified Birc6 in sEVs

1 Experiment ID 15 MISEV standards ✔ EM Biophysical techniques ✔ CD63 Enriched markers ✘ Negative markers ✘ Particle analysis Identified molecule mrna Identification method Microarray PubMed ID 17486113     Organism Mus musculus Homo sapiens Experiment description Exosome-mediated transfer of mRNAs and microRNAs is a novel mechanism of genetic exchange between cells. Authors "Valadi H, Ekstrom K, Bossios A, Sjostrand M, Lee JJ, Lotvall JO" Journal name NCB Publication year 2007 Sample Mast cells Sample name MC9 Bone marrow-derived mast cells HMC-1 Isolation/purification methods Filtration Ultracentrifugation Sucrose density gradient Flotation density 1.11-1.21 g/mL Molecules identified in the study Protein mRNA miRNA Methods used in the study Mass spectrometry [MALDI TOF] Western blotting Microarray miRCURY LNA Array

2 Experiment ID 1117 MISEV standards ✘ Biophysical techniques ✔ Cd81|Icam1 Enriched markers ✘ Negative markers ✔ NTA Particle analysis Identified molecule protein Identification method Mass spectrometry PubMed ID 34957415     Organism Mus musculus Experiment description Melanoma-derived small extracellular vesicles induce lymphangiogenesis and metastasis through an NGFR-dependent mechanism Authors "García-Silva S, Benito-Martín A, Nogués L, Hernández-Barranco A, Mazariegos MS, Santos V, Hergueta-Redondo M, Ximénez-Embún P, Kataru RP, Lopez AA, Merino C, Sánchez-Redondo S, Graña-Castro O, Matei I, Nicolás-Avila JÁ, Torres-Ruiz R, Rodríguez-Perales S, Martínez L, Pérez-Martínez M, Mata G, Szumera-Ciećkiewicz A, Kalinowska I, Saltari A, Martínez-Gómez JM, Hogan SA, Saragovi HU, Ortega S, Garcia-Martin C, Boskovic J, Levesque MP, Rutkowski P, Hidalgo A, Muñoz J, Megías D, Mehrara BJ, Lyden D, Peinado H." Journal name Nat Cancer Publication year 2021 Sample Melanoma cells Sample name B16-F1R2 Isolation/purification methods Differential centrifugation Ultracentrifugation Flotation density - Molecules identified in the study Protein Methods used in the study Western blotting Mass spectrometry

3 Experiment ID 1118 MISEV standards ✘ Biophysical techniques ✔ Cd81|Icam1 Enriched markers ✘ Negative markers ✔ NTA Particle analysis Identified molecule protein Identification method Mass spectrometry PubMed ID 34957415     Organism Mus musculus Experiment description Melanoma-derived small extracellular vesicles induce lymphangiogenesis and metastasis through an NGFR-dependent mechanism Authors "García-Silva S, Benito-Martín A, Nogués L, Hernández-Barranco A, Mazariegos MS, Santos V, Hergueta-Redondo M, Ximénez-Embún P, Kataru RP, Lopez AA, Merino C, Sánchez-Redondo S, Graña-Castro O, Matei I, Nicolás-Avila JÁ, Torres-Ruiz R, Rodríguez-Perales S, Martínez L, Pérez-Martínez M, Mata G, Szumera-Ciećkiewicz A, Kalinowska I, Saltari A, Martínez-Gómez JM, Hogan SA, Saragovi HU, Ortega S, Garcia-Martin C, Boskovic J, Levesque MP, Rutkowski P, Hidalgo A, Muñoz J, Megías D, Mehrara BJ, Lyden D, Peinado H." Journal name Nat Cancer Publication year 2021 Sample Melanoma cells Sample name B16-F1R2 Isolation/purification methods Differential centrifugation Ultracentrifugation Flotation density - Molecules identified in the study Protein Methods used in the study Western blotting Mass spectrometry

Protein-protein interactions for Birc6

Protein Interactor ExoCarta ID Identification method PubMed Species 1 Nf1   Co-fractionation Mus musculus 2 Tmem173   Proximity Label-MS Mus musculus 3 Eed   Affinity Capture-MS Mus musculus 4 Son   Co-fractionation Mus musculus 5 4932438A13Rik   Co-fractionation Mus musculus 6 Vps13b   Co-fractionation Mus musculus 7 Unk   Affinity Capture-RNA Mus musculus 8 Rnf41   Affinity Capture-Western Mus musculus View the network

Pathways in which Birc6 is involved

No pathways found