Gene description for TDRD9
Gene name tudor domain containing 9
Gene symbol TDRD9
Other names/aliases C14orf75
HIG-1
NET54
Species Homo sapiens
 Database cross references - TDRD9
ExoCarta ExoCarta_122402
Vesiclepedia VP_122402
Entrez Gene 122402
HGNC 20122
UniProt Q8NDG6  
 TDRD9 identified in sEVs derived from the following tissue/cell type
Hepatocellular carcinoma cells 26054723    
 Gene ontology annotations for TDRD9
Molecular Function
    DNA clamp loader activity GO:0003689 IEA
    RNA binding GO:0003723 IBA
    RNA helicase activity GO:0003724 IEA
    helicase activity GO:0004386 IBA
    ATP binding GO:0005524 IEA
    ATP hydrolysis activity GO:0016887 ISS
    cohesin loader activity GO:0061775 IEA
    chromatin extrusion motor activity GO:0140584 IEA
    ATP-dependent H3-H4 histone complex chaperone activity GO:0140665 IEA
    ATP-dependent H2AZ histone chaperone activity GO:0140849 IEA
Biological Process
    male meiotic nuclear division GO:0007140 ISS
    male meiosis I GO:0007141 ISS
    spermatogenesis GO:0007283 IMP
    spermatogenesis GO:0007283 ISS
    fertilization GO:0009566 ISS
    cell differentiation GO:0030154 IEA
    piRNA processing GO:0034587 ISS
    chromatin looping GO:0140588 IEA
    transposable element silencing by piRNA-mediated heterochromatin formation GO:0141006 ISS
    transposable element silencing by piRNA-mediated DNA methylation GO:0141196 ISS
Subcellular Localization
    nucleus GO:0005634 IBA
    nucleus GO:0005634 IDA
    cytoplasm GO:0005737 IBA
    cytoplasm GO:0005737 IDA
    piP-body GO:0071547 ISS
 Experiment description of studies that identified TDRD9 in sEVs
1
Experiment ID 235
MISEV standards
EM
Biophysical techniques
TSG101|Alix|HSC70|GAPDH
Enriched markers
HSP90B1
Negative markers
qNano
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors "He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N"
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocellular carcinoma cells
Sample name HKCI-8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RT-PCR
RNA Sequencing
 Protein-protein interactions for TDRD9
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 DNAJC7 7266
Affinity Capture-MS Homo sapiens
2 CLOCK  
Affinity Capture-MS Homo sapiens
3 Sf3b2  
Two-hybrid Mus musculus
4 CLK1  
Two-hybrid Homo sapiens
5 PPP2R1A 5518
Affinity Capture-MS Homo sapiens
6 SEMA4B  
Affinity Capture-MS Homo sapiens
View the network image/svg+xml
 Pathways in which TDRD9 is involved
PathwayEvidenceSource
Gene expression (Transcription) IEA Reactome
Gene Silencing by RNA IEA Reactome
PIWI-interacting RNA (piRNA) biogenesis IEA Reactome





Perform bioinformatics analysis of your extracellular vesicle data set using FunRich, a open access standalone tool. NEW UPDATED VERSION OF FunRich available for download (12/09/2016) from here