Gene description for C3
Gene name complement component 3
Gene symbol C3
Other names/aliases AI255234
ASP
HSE-MSF
Plp
Species Mus musculus
 Database cross references - C3
ExoCarta ExoCarta_12266
Entrez Gene 12266
UniProt P01027  
 C3 identified in exosomes derived from the following tissue/cell type
Macrophages 23658846    
Macrophages 23658846    
Macrophages 23658846    
Mov neuroglial cells 15210972    
 Gene ontology annotations for C3
Molecular Function
    protein binding GO:0005515 IPI
    cofactor binding GO:0048037 ISO
    endopeptidase inhibitor activity GO:0004866 IEA
    lipid binding GO:0008289 ISO
    C5L2 anaphylatoxin chemotactic receptor binding GO:0031715 ISO
Biological Process
    positive regulation of angiogenesis GO:0045766 IMP
    complement activation, classical pathway GO:0006958 IEA
    positive regulation of activation of membrane attack complex GO:0001970 IMP
    positive regulation of developmental growth GO:0048639 ISO
    innate immune response GO:0045087 IEA
    regulation of triglyceride biosynthetic process GO:0010866 ISO
    inflammatory response GO:0006954 IEA
    complement activation, alternative pathway GO:0006957 IEA
    positive regulation of apoptotic cell clearance GO:2000427 ISO
    positive regulation of type IIa hypersensitivity GO:0001798 IMP
    positive regulation of vascular endothelial growth factor production GO:0010575 ISO
    blood coagulation GO:0007596 ISO
    positive regulation of G-protein coupled receptor protein signaling pathway GO:0045745 ISO
    immune system process GO:0002376 IEA
    lipid metabolic process GO:0006629 IEA
    positive regulation of ERK1 and ERK2 cascade GO:0070374 ISO
    positive regulation of lipid storage GO:0010884 ISO
    positive regulation of protein phosphorylation GO:0001934 ISO
    positive regulation of phagocytosis GO:0050766 IMP
    positive regulation of glucose transport GO:0010828 ISO
    complement activation GO:0006956 ISO
    fatty acid metabolic process GO:0006631 IEA
Subcellular Localization
    blood microparticle GO:0072562 ISO
    extracellular exosome GO:0070062 ISO
    extracellular region GO:0005576 IEA
    extracellular space GO:0005615 ISO
 Experiment description of studies that identified C3 in exosomes
1
Experiment ID 214
ISEV standards
✔
EM
EV Biophysical techniques
✘ EV Cytosolic markers
✘ EV Membrane markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23658846    
Organism Mus musculus
Experiment description Immunomodulatory impact of leishmania-induced macrophage exosomes: a comparative proteomic and functional analysis.
Authors Hassani K, Olivier M.
Journal name PLoS Negl Trop Dis
Publication year 2013
Sample Macrophages
Sample name Leishmania-infected-Macrophage (J774A.1)
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Filtration
Protease inhibitors
Sucrose density gradient
Filtration
Ultracentrifugation
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
2
Experiment ID 215
ISEV standards
✔
EM
EV Biophysical techniques
✘ EV Cytosolic markers
✘ EV Membrane markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23658846    
Organism Mus musculus
Experiment description Immunomodulatory impact of leishmania-induced macrophage exosomes: a comparative proteomic and functional analysis.
Authors Hassani K, Olivier M.
Journal name PLoS Negl Trop Dis
Publication year 2013
Sample Macrophages
Sample name LPS-treated-Macrophage (J774A.1)
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Filtration
Protease inhibitors
Sucrose density gradient
Filtration
Ultracentrifugation
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
3
Experiment ID 216
ISEV standards
✔
EM
EV Biophysical techniques
✘ EV Cytosolic markers
✘ EV Membrane markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23658846    
Organism Mus musculus
Experiment description Immunomodulatory impact of leishmania-induced macrophage exosomes: a comparative proteomic and functional analysis.
Authors Hassani K, Olivier M.
Journal name PLoS Negl Trop Dis
Publication year 2013
Sample Macrophages
Sample name Normal-Macrophage (J774A.1)
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Filtration
Protease inhibitors
Sucrose density gradient
Filtration
Ultracentrifugation
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
4
Experiment ID 39
ISEV standards
✔
IEM
EV Biophysical techniques
✔
TSG101|HSC70
EV Cytosolic markers
✔
FLOT1
EV Membrane markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 15210972    
Organism Mus musculus
Experiment description Cells release prions in association with exosomes.
Authors Fevrier B, Vilette D, Archer F, Loew D, Faigle W, Vidal M, Laude H, Raposo G
Journal name PNAS
Publication year 2004
Sample Mov neuroglial cells
Sample name Mov neuroglial cell
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Flotation density 1.14 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [QSTAR]
Western blotting
Immunoelectron Microscopy
 Protein-protein interactions for C3
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.



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