Gene description for C3
Gene name complement component 3
Gene symbol C3
Other names/aliases AI255234
ASP
HSE-MSF
Plp
Species Mus musculus
 Database cross references - C3
ExoCarta ExoCarta_12266
Vesiclepedia VP_12266
Entrez Gene 12266
UniProt P01027  
 C3 identified in sEVs derived from the following tissue/cell type
Colon cancer cells 37309723    
Faeces 33431899    
Faeces 33431899    
Macrophages 23658846    
Macrophages 23658846    
Macrophages 23658846    
Mov neuroglial cells 15210972    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for C3
Molecular Function
    endopeptidase inhibitor activity GO:0004866 IEA
    protein binding GO:0005515 IPI
    lipid binding GO:0008289 ISO
    C5L2 anaphylatoxin chemotactic receptor binding GO:0031715 ISO
    C5L2 anaphylatoxin chemotactic receptor binding GO:0031715 ISS
    receptor ligand activity GO:0048018 IEA
Biological Process
    positive regulation of type IIa hypersensitivity GO:0001798 IMP
    positive regulation of protein phosphorylation GO:0001934 ISO
    positive regulation of protein phosphorylation GO:0001934 ISS
    positive regulation of activation of membrane attack complex GO:0001970 IMP
    complement receptor mediated signaling pathway GO:0002430 IMP
    fatty acid metabolic process GO:0006631 IEA
    inflammatory response GO:0006954 IEA
    complement activation GO:0006956 IBA
    complement activation GO:0006956 IDA
    complement activation GO:0006956 IMP
    complement activation GO:0006956 ISO
    complement activation, alternative pathway GO:0006957 IEA
    complement activation, classical pathway GO:0006958 IEA
    response to bacterium GO:0009617 IEP
    positive regulation of vascular endothelial growth factor production GO:0010575 IEA
    positive regulation of vascular endothelial growth factor production GO:0010575 ISO
    positive regulation of D-glucose transmembrane transport GO:0010828 ISO
    positive regulation of D-glucose transmembrane transport GO:0010828 ISS
    regulation of triglyceride biosynthetic process GO:0010866 ISO
    regulation of triglyceride biosynthetic process GO:0010866 ISS
    positive regulation of lipid storage GO:0010884 ISO
    positive regulation of lipid storage GO:0010884 ISS
    neuron remodeling GO:0016322 IMP
    oviduct epithelium development GO:0035846 IGI
    vascular associated smooth muscle cell differentiation GO:0035886 IGI
    B cell activation GO:0042113 IEA
    positive regulation of G protein-coupled receptor signaling pathway GO:0045745 ISO
    positive regulation of G protein-coupled receptor signaling pathway GO:0045745 ISS
    positive regulation of angiogenesis GO:0045766 IMP
    positive regulation of receptor-mediated endocytosis GO:0048260 IGI
    positive regulation of receptor-mediated endocytosis GO:0048260 IMP
    positive regulation of developmental growth GO:0048639 ISO
    positive regulation of phagocytosis GO:0050766 IMP
    retina development in camera-type eye GO:0060041 IGI
    positive regulation of phagocytosis, engulfment GO:0060100 IGI
    positive regulation of phagocytosis, engulfment GO:0060100 IMP
    positive regulation of ERK1 and ERK2 cascade GO:0070374 ISO
    amyloid-beta clearance GO:0097242 IGI
    amyloid-beta clearance GO:0097242 IMP
    complement-dependent cytotoxicity GO:0097278 IMP
    complement-mediated synapse pruning GO:0150062 IMP
    vertebrate eye-specific patterning GO:0150064 IMP
    positive regulation of apoptotic cell clearance GO:2000427 IEA
    positive regulation of apoptotic cell clearance GO:2000427 ISO
Subcellular Localization
    extracellular region GO:0005576 IDA
    extracellular space GO:0005615 HDA
    extracellular space GO:0005615 IBA
    extracellular space GO:0005615 IDA
    extracellular space GO:0005615 ISO
    cell surface GO:0009986 IDA
    protein-containing complex GO:0032991 IDA
 Experiment description of studies that identified C3 in sEVs
1
Experiment ID 907
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 303
MISEV standards
✔
EM
Biophysical techniques
✔
Cd63|Cd81|Cd9
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33431899    
Organism Mus musculus
Experiment description High-fat diet-induced upregulation of exosomal phosphatidylcholine contributes to insulin resistance
Authors "Kumar A, Sundaram K, Mu J, Dryden GW, Sriwastva MK, Lei C, Zhang L, Qiu X, Xu F, Yan J, Zhang X, Park JW, Merchant ML, Bohler HCL, Wang B, Zhang S, Qin C, Xu Z, Han X, McClain CJ, Teng Y, Zhang HG."
Journal name Nat Commun
Publication year 2021
Sample Faeces
Sample name Intestinal epithelial cells - C57BL/6
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient centrifugation
Flotation density -
Molecules identified in the study Protein
Lipid
miRNA
Methods used in the study Western blotting
Mass spectrometry
Immunofluorescence
miRNA array
HPLC
3
Experiment ID 304
MISEV standards
✔
EM
Biophysical techniques
✔
Cd63|Cd81|Cd9
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33431899    
Organism Mus musculus
Experiment description High-fat diet-induced upregulation of exosomal phosphatidylcholine contributes to insulin resistance
Authors "Kumar A, Sundaram K, Mu J, Dryden GW, Sriwastva MK, Lei C, Zhang L, Qiu X, Xu F, Yan J, Zhang X, Park JW, Merchant ML, Bohler HCL, Wang B, Zhang S, Qin C, Xu Z, Han X, McClain CJ, Teng Y, Zhang HG."
Journal name Nat Commun
Publication year 2021
Sample Faeces
Sample name Intestinal epithelial cells - C57BL/6
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient centrifugation
Flotation density -
Molecules identified in the study Protein
Lipid
miRNA
Methods used in the study Western blotting
Mass spectrometry
Immunofluorescence
miRNA array
HPLC
4
Experiment ID 214
MISEV standards
✔
EM
Biophysical techniques
✘ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23658846    
Organism Mus musculus
Experiment description Immunomodulatory impact of leishmania-induced macrophage exosomes: a comparative proteomic and functional analysis.
Authors "Hassani K, Olivier M."
Journal name PLoS Negl Trop Dis
Publication year 2013
Sample Macrophages
Sample name Leishmania-infected-Macrophage (J774A.1)
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Filtration
Protease inhibitors
Sucrose density gradient
Filtration
Ultracentrifugation
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
5
Experiment ID 215
MISEV standards
✔
EM
Biophysical techniques
✘ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23658846    
Organism Mus musculus
Experiment description Immunomodulatory impact of leishmania-induced macrophage exosomes: a comparative proteomic and functional analysis.
Authors "Hassani K, Olivier M."
Journal name PLoS Negl Trop Dis
Publication year 2013
Sample Macrophages
Sample name LPS-treated-Macrophage (J774A.1)
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Filtration
Protease inhibitors
Sucrose density gradient
Filtration
Ultracentrifugation
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
6
Experiment ID 216
MISEV standards
✔
EM
Biophysical techniques
✘ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23658846    
Organism Mus musculus
Experiment description Immunomodulatory impact of leishmania-induced macrophage exosomes: a comparative proteomic and functional analysis.
Authors "Hassani K, Olivier M."
Journal name PLoS Negl Trop Dis
Publication year 2013
Sample Macrophages
Sample name Normal-Macrophage (J774A.1)
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Filtration
Protease inhibitors
Sucrose density gradient
Filtration
Ultracentrifugation
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
7
Experiment ID 39
MISEV standards
✔
IEM
Biophysical techniques
✔
TSG101|HSC70|FLOT1
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 15210972    
Organism Mus musculus
Experiment description Cells release prions in association with exosomes.
Authors "Fevrier B, Vilette D, Archer F, Loew D, Faigle W, Vidal M, Laude H, Raposo G"
Journal name PNAS
Publication year 2004
Sample Mov neuroglial cells
Sample name Mov neuroglial cell
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Flotation density 1.14 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [QSTAR]
Western blotting
Immunoelectron Microscopy
8
Experiment ID 908
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for C3
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Fancd2  
Affinity Capture-MS Mus musculus
2 Nphp1  
Affinity Capture-MS Mus musculus
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