Gene description for Capn2
Gene name calpain 2
Gene symbol Capn2
Other names/aliases AI326419
Capa-2
Capa2
m-calpain
m-calpin
Species Mus musculus
 Database cross references - Capn2
ExoCarta ExoCarta_12334
Vesiclepedia VP_12334
Entrez Gene 12334
UniProt O08529  
 Capn2 identified in exosomes derived from the following tissue/cell type
Colon cancer cells 37309723    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Capn2
Molecular Function
    calcium-dependent cysteine-type endopeptidase activity GO:0004198 TAS
    hydrolase activity GO:0016787 IEA
    cytoskeletal protein binding GO:0008092 ISO
    metal ion binding GO:0046872 IEA
    calcium ion binding GO:0005509 ISO
    peptidase activity GO:0008233 IDA
    cysteine-type peptidase activity GO:0008234 IEA
    protein heterodimerization activity GO:0046982 ISO
Biological Process
    cellular response to amino acid stimulus GO:0071230 ISO
    blastocyst development GO:0001824 IMP
    proteolysis GO:0006508 TAS
    protein autoprocessing GO:0016540 ISO
    regulation of cytoskeleton organization GO:0051493 IEA
    myoblast fusion GO:0007520 IMP
Subcellular Localization
    cytosol GO:0005829 ISO
    extracellular exosome GO:0070062 ISO
    pseudopodium GO:0031143 ISO
    dendrite GO:0030425 ISO
    focal adhesion GO:0005925 ISO
    membrane raft GO:0045121 ISO
    chromatin GO:0000785 IDA
    endoplasmic reticulum GO:0005783 ISO
    lysosome GO:0005764 IDA
    nucleus GO:0005634 IDA
    plasma membrane GO:0005886 ISO
    cytoplasm GO:0005737 ISO
    membrane GO:0016020 IEA
    Golgi apparatus GO:0005794 ISO
    perinuclear endoplasmic reticulum GO:0097038 ISO
    intracellular GO:0005622 IEA
 Experiment description of studies that identified Capn2 in exosomes
1
Experiment ID 907
MISEV standards
✔
EM
EV Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
EV Enriched markers
✔
HSPA5
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S.
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 908
MISEV standards
✔
EM
EV Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
EV Enriched markers
✔
HSPA5
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S.
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Capn2
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.
 Pathways in which Capn2 is involved
No pathways found





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