Gene description for Capn2
Gene name calpain 2
Gene symbol Capn2
Other names/aliases AI326419
Capa-2
Capa2
m-calpain
m-calpin
Species Mus musculus
 Database cross references - Capn2
ExoCarta ExoCarta_12334
Vesiclepedia VP_12334
Entrez Gene 12334
UniProt O08529  
 Capn2 identified in sEVs derived from the following tissue/cell type
Colon cancer cells 37309723    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Capn2
Molecular Function
    calcium-dependent cysteine-type endopeptidase activity GO:0004198 IDA
    calcium-dependent cysteine-type endopeptidase activity GO:0004198 ISO
    calcium-dependent cysteine-type endopeptidase activity GO:0004198 ISO
    calcium-dependent cysteine-type endopeptidase activity GO:0004198 TAS
    calcium ion binding GO:0005509 IEA
    calcium ion binding GO:0005509 ISO
    cytoskeletal protein binding GO:0008092 IEA
    cytoskeletal protein binding GO:0008092 ISO
    peptidase activity GO:0008233 IDA
    enzyme binding GO:0019899 IEA
    enzyme binding GO:0019899 ISO
    protein-containing complex binding GO:0044877 IEA
    protein-containing complex binding GO:0044877 ISO
Biological Process
    response to hypoxia GO:0001666 IEA
    blastocyst development GO:0001824 IMP
    proteolysis GO:0006508 IBA
    proteolysis GO:0006508 IDA
    proteolysis GO:0006508 ISO
    proteolysis GO:0006508 NAS
    myoblast fusion GO:0007520 IMP
    female pregnancy GO:0007565 IEA
    response to mechanical stimulus GO:0009612 IEA
    positive regulation of cardiac muscle cell apoptotic process GO:0010666 IEA
    positive regulation of cardiac muscle cell apoptotic process GO:0010666 ISO
    protein autoprocessing GO:0016540 IEA
    protein autoprocessing GO:0016540 ISO
    protein catabolic process GO:0030163 ISO
    regulation of interleukin-6 production GO:0032675 IEA
    regulation of interleukin-6 production GO:0032675 ISO
    cellular response to interferon-beta GO:0035458 IEA
    response to hydrogen peroxide GO:0042542 IEA
    behavioral response to pain GO:0048266 IEA
    behavioral response to pain GO:0048266 ISO
    synaptic vesicle endocytosis GO:0048488 EXP
    synaptic vesicle endocytosis GO:0048488 IDA
    synaptic vesicle endocytosis GO:0048488 IEP
    synaptic vesicle endocytosis GO:0048488 IMP
    proteolysis involved in protein catabolic process GO:0051603 IEA
    proteolysis involved in protein catabolic process GO:0051603 ISO
    cellular response to lipopolysaccharide GO:0071222 IEA
    cellular response to amino acid stimulus GO:0071230 IDA
    cellular response to amino acid stimulus GO:0071230 ISO
    protein catabolic process at postsynapse GO:0140249 EXP
    protein catabolic process at postsynapse GO:0140249 IDA
    protein catabolic process at postsynapse GO:0140249 IEP
    protein catabolic process at postsynapse GO:0140249 IMP
    positive regulation of myoblast fusion GO:1901741 IEA
    positive regulation of myoblast fusion GO:1901741 ISO
    positive regulation of phosphatidylcholine biosynthetic process GO:2001247 IEA
    positive regulation of phosphatidylcholine biosynthetic process GO:2001247 ISO
Subcellular Localization
    chromatin GO:0000785 IDA
    nucleus GO:0005634 IDA
    cytoplasm GO:0005737 IBA
    cytoplasm GO:0005737 IDA
    cytoplasm GO:0005737 ISO
    lysosome GO:0005764 IDA
    endoplasmic reticulum GO:0005783 ISO
    Golgi apparatus GO:0005794 IEA
    Golgi apparatus GO:0005794 ISO
    cytosol GO:0005829 IDA
    cytosol GO:0005829 ISO
    cytosol GO:0005829 ISS
    plasma membrane GO:0005886 ISO
    plasma membrane GO:0005886 ISS
    plasma membrane GO:0005886 TAS
    focal adhesion GO:0005925 IEA
    focal adhesion GO:0005925 ISO
    external side of plasma membrane GO:0009897 IEA
    external side of plasma membrane GO:0009897 ISO
    dendrite GO:0030425 IEA
    dendrite GO:0030425 ISO
    pseudopodium GO:0031143 IEA
    pseudopodium GO:0031143 ISO
    cell projection GO:0042995 ISO
    neuronal cell body GO:0043025 IEA
    neuronal cell body GO:0043025 ISO
    membrane raft GO:0045121 ISO
    membrane raft GO:0045121 ISO
    membrane raft GO:0045121 ISS
    perinuclear endoplasmic reticulum GO:0097038 IEA
    perinuclear endoplasmic reticulum GO:0097038 ISO
    presynapse GO:0098793 IEA
    postsynapse GO:0098794 IDA
    postsynapse GO:0098794 IMP
    calpain complex GO:0110158 ISO
 Experiment description of studies that identified Capn2 in sEVs
1
Experiment ID 907
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 908
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Capn2
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Uba3  
Co-fractionation Mus musculus
2 Nae1  
Co-fractionation Mus musculus
3 Cth  
Co-fractionation Mus musculus
4 Mapk1 26413
Affinity Capture-Western Mus musculus
Affinity Capture-Western Mus musculus
5 Eed  
Affinity Capture-MS Mus musculus
6 Tln1 21894
Affinity Capture-Western Mus musculus
7 MAPT  
Reconstituted Complex Homo sapiens
8 Twf2  
Co-fractionation Mus musculus
9 Tatdn1  
Co-fractionation Mus musculus
10 Rapsn  
Affinity Capture-Western Mus musculus
View the network image/svg+xml



Perform bioinformatics analysis of your extracellular vesicle data set using FunRich, a open access standalone tool. NEW UPDATED VERSION OF FunRich available for download (12/09/2016) from here