Gene description for Casp8
Gene name caspase 8
Gene symbol Casp8
Other names/aliases CASP-8
FLICE
MACH
Mch5
Species Mus musculus
 Database cross references - Casp8
ExoCarta ExoCarta_12370
Entrez Gene 12370
UniProt O89110  
 Casp8 identified in exosomes derived from the following tissue/cell type
Mast cells 17486113    
 Gene ontology annotations for Casp8
Molecular Function
    cysteine-type endopeptidase activity involved in apoptotic signaling pathway GO:0097199 ISO
    cysteine-type endopeptidase activity involved in apoptotic process GO:0097153 ISO
    cysteine-type endopeptidase activity GO:0004197 ISO
    endopeptidase activity GO:0004175 IMP
    scaffold protein binding GO:0097110 ISO
    hydrolase activity GO:0016787 IEA
    protein heterodimerization activity GO:0046982 IPI
    cysteine-type peptidase activity GO:0008234 IEA
    ubiquitin protein ligase binding GO:0031625 ISO
    protein binding GO:0005515 IPI
    tumor necrosis factor receptor binding GO:0005164 ISO
    death receptor binding GO:0005123 ISO
    protein complex binding GO:0032403 ISO
    death effector domain binding GO:0035877 ISO
    peptidase activity GO:0008233 ISO
Biological Process
    positive regulation of I-kappaB kinase/NF-kappaB signaling GO:0043123 ISO
    extrinsic apoptotic signaling pathway via death domain receptors GO:0008625 IMP
    extrinsic apoptotic signaling pathway GO:0097191 ISO
    angiogenesis GO:0001525 IMP
    neural tube formation GO:0001841 IMP
    response to ethanol GO:0045471 ISO
    proteolysis involved in cellular protein catabolic process GO:0051603 ISO
    negative regulation of necroptotic process GO:0060546 IGI
    macrophage differentiation GO:0030225 TAS
    proteolysis GO:0006508 ISO
    regulation of apoptotic process GO:0042981 IEA
    cardiac muscle tissue development GO:0048738 TAS
    regulation of apoptotic signaling pathway GO:2001233 IGI
    positive regulation of macrophage differentiation GO:0045651 ISO
    activation of cysteine-type endopeptidase activity GO:0097202 ISO
    apoptotic process GO:0006915 ISO
    negative regulation of I-kappaB kinase/NF-kappaB signaling GO:0043124 ISO
    TRAIL-activated apoptotic signaling pathway GO:0036462 ISO
    positive regulation of proteolysis GO:0045862 ISO
    apoptotic signaling pathway GO:0097190 ISO
    hepatocyte apoptotic process GO:0097284 IMP
    regulation of thymocyte apoptotic process GO:0070243 IGI
    heart development GO:0007507 IMP
    positive regulation of extrinsic apoptotic signaling pathway GO:2001238 IGI
    execution phase of apoptosis GO:0097194 ISO
    protein heterooligomerization GO:0051291 ISO
    response to tumor necrosis factor GO:0034612 ISO
    activation of cysteine-type endopeptidase activity involved in apoptotic process GO:0006919 IDA
Subcellular Localization
    membrane raft GO:0045121 ISO
    nucleus GO:0005634 IDA
    CD95 death-inducing signaling complex GO:0031265 ISO
    mitochondrion GO:0005739 ISO
    Noc1p-Noc2p complex GO:0030690 IMP
    nucleoplasm GO:0005654 ISO
    neuron projection GO:0043005 ISO
    cytoplasm GO:0005737 ISO
    protein complex GO:0043234 ISO
    cytosol GO:0005829 ISO
    cell body GO:0044297 ISO
    plasma membrane GO:0005886 IDA
    microtubule organizing center GO:0005815 ISO
    death-inducing signaling complex GO:0031264 ISO
    ripoptosome GO:0097342 ISO
 Experiment description of studies that identified Casp8 in exosomes
1
Experiment ID 15
ISEV standards
EM
EV Biophysical techniques
EV Cytosolic markers
CD63
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 17486113    
Organism Mus musculus
Homo sapiens
Experiment description Exosome-mediated transfer of mRNAs and microRNAs is a novel mechanism of genetic exchange between cells.
Authors Valadi H, Ekstrom K, Bossios A, Sjostrand M, Lee JJ, Lotvall JO
Journal name NCB
Publication year 2007
Sample Mast cells
Sample name MC9
Bone marrow-derived mast cells
HMC-1
Isolation/purification methods Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.11-1.21 g/mL
Molecules identified in the study Protein
mRNA
miRNA
Methods used in the study Mass spectrometry [MALDI TOF]
Western blotting
Microarray
miRCURY LNA Array
2
Experiment ID 15
ISEV standards
EM
EV Biophysical techniques
EV Cytosolic markers
CD63
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule mrna
Identification method Microarray
PubMed ID 17486113    
Organism Mus musculus
Homo sapiens
Experiment description Exosome-mediated transfer of mRNAs and microRNAs is a novel mechanism of genetic exchange between cells.
Authors Valadi H, Ekstrom K, Bossios A, Sjostrand M, Lee JJ, Lotvall JO
Journal name NCB
Publication year 2007
Sample Mast cells
Sample name MC9
Bone marrow-derived mast cells
HMC-1
Isolation/purification methods Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.11-1.21 g/mL
Molecules identified in the study Protein
mRNA
miRNA
Methods used in the study Mass spectrometry [MALDI TOF]
Western blotting
Microarray
miRCURY LNA Array
 Protein-protein interactions for Casp8
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.



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