Gene description for Cd14
Gene name CD14 antigen
Gene symbol Cd14
Other names/aliases -
Species Mus musculus
 Database cross references - Cd14
ExoCarta ExoCarta_12475
Entrez Gene 12475
UniProt P10810  
 Cd14 identified in exosomes derived from the following tissue/cell type
Macrophages 23658846    
Macrophages 23658846    
Macrophages 23658846    
Microglia 16081791    
 Gene ontology annotations for Cd14
Molecular Function
    lipoteichoic acid binding GO:0070891 ISO
    lipopolysaccharide binding GO:0001530 ISO
Biological Process
    innate immune response GO:0045087 IEA
    positive regulation of cytokine secretion GO:0050715 ISO
    positive regulation of endocytosis GO:0045807 IMP
    cellular response to lipopolysaccharide GO:0071222 ISO
    response to molecule of bacterial origin GO:0002237 IDA
    cellular response to lipoteichoic acid GO:0071223 ISO
    positive regulation of tumor necrosis factor production GO:0032760 ISO
    immune system process GO:0002376 IEA
    inflammatory response GO:0006954 ISO
    response to tumor necrosis factor GO:0034612 ISO
Subcellular Localization
    membrane GO:0016020 IEA
    extracellular exosome GO:0070062 ISO
    plasma membrane GO:0005886 ISO
    cell surface GO:0009986 ISO
    membrane raft GO:0045121 IDA
    anchored component of membrane GO:0031225 IEA
    extracellular space GO:0005615 ISO
 Experiment description of studies that identified Cd14 in exosomes
1
Experiment ID 214
ISEV standards
✔
EM
EV Biophysical techniques
✘ EV Cytosolic markers
✘ EV Membrane markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23658846    
Organism Mus musculus
Experiment description Immunomodulatory impact of leishmania-induced macrophage exosomes: a comparative proteomic and functional analysis.
Authors Hassani K, Olivier M.
Journal name PLoS Negl Trop Dis
Publication year 2013
Sample Macrophages
Sample name Leishmania-infected-Macrophage (J774A.1)
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Filtration
Protease inhibitors
Sucrose density gradient
Filtration
Ultracentrifugation
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
2
Experiment ID 215
ISEV standards
✔
EM
EV Biophysical techniques
✘ EV Cytosolic markers
✘ EV Membrane markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23658846    
Organism Mus musculus
Experiment description Immunomodulatory impact of leishmania-induced macrophage exosomes: a comparative proteomic and functional analysis.
Authors Hassani K, Olivier M.
Journal name PLoS Negl Trop Dis
Publication year 2013
Sample Macrophages
Sample name LPS-treated-Macrophage (J774A.1)
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Filtration
Protease inhibitors
Sucrose density gradient
Filtration
Ultracentrifugation
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
3
Experiment ID 216
ISEV standards
✔
EM
EV Biophysical techniques
✘ EV Cytosolic markers
✘ EV Membrane markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23658846    
Organism Mus musculus
Experiment description Immunomodulatory impact of leishmania-induced macrophage exosomes: a comparative proteomic and functional analysis.
Authors Hassani K, Olivier M.
Journal name PLoS Negl Trop Dis
Publication year 2013
Sample Macrophages
Sample name Normal-Macrophage (J774A.1)
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Filtration
Protease inhibitors
Sucrose density gradient
Filtration
Ultracentrifugation
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
4
Experiment ID 14
ISEV standards
✔
EM
EV Biophysical techniques
✔
RAB7|RAB11
EV Cytosolic markers
✔
CD9|CD63|LAMP1|LAMP2
EV Membrane markers
✔
DNM
EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 16081791    
Organism Mus musculus
Experiment description Proteomic analysis of microglia-derived exosomes: metabolic role of the aminopeptidase CD13 in neuropeptide catabolism.
Authors Potolicchio I, Carven GJ, Xu X, Stipp C, Riese RJ, Stern LJ, Santambrogio L
Journal name JIMMU
Publication year 2005
Sample Microglia
Sample name N9
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Flotation density 1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [LCQ DECA XP]
Western blotting
 Protein-protein interactions for Cd14
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Tlr4  
Affinity Capture-Western Mus musculus
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