Gene description for Cd14
Gene name CD14 antigen
Gene symbol Cd14
Other names/aliases -
Species Mus musculus
 Database cross references - Cd14
ExoCarta ExoCarta_12475
Vesiclepedia VP_12475
Entrez Gene 12475
UniProt P10810  
 Cd14 identified in sEVs derived from the following tissue/cell type
Colon cancer cells 37309723    
Macrophages 23658846    
Macrophages 23658846    
Macrophages 23658846    
Microglia 16081791    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Cd14
Molecular Function
    lipopolysaccharide binding GO:0001530 IBA
    lipopolysaccharide binding GO:0001530 ISO
    lipopolysaccharide immune receptor activity GO:0001875 IEA
    lipopolysaccharide immune receptor activity GO:0001875 ISO
    lipoteichoic acid binding GO:0070891 ISO
Biological Process
    positive regulation of cytokine production GO:0001819 IBA
    positive regulation of cytokine production GO:0001819 ISO
    response to molecule of bacterial origin GO:0002237 IDA
    cell surface pattern recognition receptor signaling pathway GO:0002752 ISO
    receptor-mediated endocytosis GO:0006898 IMP
    inflammatory response GO:0006954 IEA
    response to bacterium GO:0009617 IEP
    response to bacterium GO:0009617 IMP
    lipopolysaccharide-mediated signaling pathway GO:0031663 IEA
    positive regulation of lipopolysaccharide-mediated signaling pathway GO:0031666 IMP
    response to magnesium ion GO:0032026 IEA
    positive regulation of type I interferon production GO:0032481 IMP
    positive regulation of type II interferon production GO:0032729 IMP
    positive regulation of interleukin-8 production GO:0032757 IEA
    positive regulation of interleukin-8 production GO:0032757 ISO
    positive regulation of tumor necrosis factor production GO:0032760 IMP
    positive regulation of tumor necrosis factor production GO:0032760 ISO
    positive regulation of tumor necrosis factor production GO:0032760 ISO
    toll-like receptor 4 signaling pathway GO:0034142 IBA
    toll-like receptor 4 signaling pathway GO:0034142 IEA
    toll-like receptor 4 signaling pathway GO:0034142 ISO
    positive regulation of toll-like receptor 4 signaling pathway GO:0034145 IMP
    response to tumor necrosis factor GO:0034612 IEA
    response to tumor necrosis factor GO:0034612 ISO
    innate immune response GO:0045087 IEA
    response to ethanol GO:0045471 IEA
    positive regulation of endocytosis GO:0045807 IMP
    response to electrical stimulus GO:0051602 IEA
    cellular response to molecule of bacterial origin GO:0071219 ISO
    cellular response to lipopolysaccharide GO:0071222 IBA
    cellular response to lipopolysaccharide GO:0071222 IMP
    cellular response to lipopolysaccharide GO:0071222 ISO
    cellular response to lipopolysaccharide GO:0071222 ISO
    cellular response to lipoteichoic acid GO:0071223 ISO
    cellular response to diacyl bacterial lipopeptide GO:0071726 ISO
    cellular response to diacyl bacterial lipopeptide GO:0071726 ISS
    cellular response to triacyl bacterial lipopeptide GO:0071727 ISO
    cellular response to triacyl bacterial lipopeptide GO:0071727 ISS
Subcellular Localization
    extracellular space GO:0005615 IEA
    extracellular space GO:0005615 ISO
    extracellular space GO:0005615 ISO
    Golgi apparatus GO:0005794 ISO
    Golgi apparatus GO:0005794 ISS
    external side of plasma membrane GO:0009897 IBA
    external side of plasma membrane GO:0009897 IDA
    external side of plasma membrane GO:0009897 ISO
    external side of plasma membrane GO:0009897 ISS
    cell surface GO:0009986 ISO
    membrane raft GO:0045121 IBA
    membrane raft GO:0045121 IDA
    membrane raft GO:0045121 ISO
    lipopolysaccharide receptor complex GO:0046696 IBA
    lipopolysaccharide receptor complex GO:0046696 ISO
    lipopolysaccharide receptor complex GO:0046696 ISS
 Experiment description of studies that identified Cd14 in sEVs
1
Experiment ID 907
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 214
MISEV standards
✔
EM
Biophysical techniques
✘ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23658846    
Organism Mus musculus
Experiment description Immunomodulatory impact of leishmania-induced macrophage exosomes: a comparative proteomic and functional analysis.
Authors "Hassani K, Olivier M."
Journal name PLoS Negl Trop Dis
Publication year 2013
Sample Macrophages
Sample name Leishmania-infected-Macrophage (J774A.1)
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Filtration
Protease inhibitors
Sucrose density gradient
Filtration
Ultracentrifugation
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
3
Experiment ID 215
MISEV standards
✔
EM
Biophysical techniques
✘ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23658846    
Organism Mus musculus
Experiment description Immunomodulatory impact of leishmania-induced macrophage exosomes: a comparative proteomic and functional analysis.
Authors "Hassani K, Olivier M."
Journal name PLoS Negl Trop Dis
Publication year 2013
Sample Macrophages
Sample name LPS-treated-Macrophage (J774A.1)
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Filtration
Protease inhibitors
Sucrose density gradient
Filtration
Ultracentrifugation
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
4
Experiment ID 216
MISEV standards
✔
EM
Biophysical techniques
✘ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23658846    
Organism Mus musculus
Experiment description Immunomodulatory impact of leishmania-induced macrophage exosomes: a comparative proteomic and functional analysis.
Authors "Hassani K, Olivier M."
Journal name PLoS Negl Trop Dis
Publication year 2013
Sample Macrophages
Sample name Normal-Macrophage (J774A.1)
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Filtration
Protease inhibitors
Sucrose density gradient
Filtration
Ultracentrifugation
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
5
Experiment ID 14
MISEV standards
✔
EM
Biophysical techniques
✔
RAB7|RAB11|CD9|CD63|LAMP1|LAMP2
Enriched markers
✔
DNM
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 16081791    
Organism Mus musculus
Experiment description Proteomic analysis of microglia-derived exosomes: metabolic role of the aminopeptidase CD13 in neuropeptide catabolism.
Authors "Potolicchio I, Carven GJ, Xu X, Stipp C, Riese RJ, Stern LJ, Santambrogio L"
Journal name JIMMU
Publication year 2005
Sample Microglia
Sample name N9
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Flotation density 1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [LCQ DECA XP]
Western blotting
6
Experiment ID 908
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Cd14
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Lpar1  
Affinity Capture-Western Mus musculus
2 Tlr4  
Affinity Capture-Western Mus musculus
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