Gene description for Cd28
Gene name CD28 antigen
Gene symbol Cd28
Other names/aliases -
Species Mus musculus
 Database cross references - Cd28
ExoCarta ExoCarta_12487
Vesiclepedia VP_12487
Entrez Gene 12487
UniProt P31041  
 Cd28 identified in sEVs derived from the following tissue/cell type
Colon cancer cells 37309723    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Cd28
Molecular Function
    protein kinase binding GO:0019901 IPI
    identical protein binding GO:0042802 ISO
Biological Process
    positive regulation of inflammatory response to antigenic stimulus GO:0002863 IMP
    transcription by RNA polymerase II GO:0006366 IGI
    immune response GO:0006955 IEA
    positive regulation of gene expression GO:0010628 ISO
    negative regulation of gene expression GO:0010629 IEA
    negative regulation of gene expression GO:0010629 ISO
    T cell costimulation GO:0031295 IBA
    T cell costimulation GO:0031295 IDA
    T cell costimulation GO:0031295 IGI
    T cell costimulation GO:0031295 IMP
    T cell costimulation GO:0031295 ISO
    interleukin-2 production GO:0032623 IMP
    positive regulation of interleukin-10 production GO:0032733 ISO
    positive regulation of interleukin-10 production GO:0032733 ISS
    positive regulation of interleukin-2 production GO:0032743 IMP
    positive regulation of interleukin-2 production GO:0032743 ISO
    positive regulation of interleukin-2 production GO:0032743 ISS
    positive regulation of interleukin-4 production GO:0032753 ISO
    positive regulation of interleukin-4 production GO:0032753 ISS
    CD4-positive, alpha-beta T cell proliferation GO:0035739 IDA
    CD4-positive, alpha-beta T cell proliferation GO:0035739 IMP
    T cell proliferation GO:0042098 IDA
    T cell proliferation GO:0042098 IMP
    positive regulation of T cell proliferation GO:0042102 IBA
    positive regulation of T cell proliferation GO:0042102 IDA
    positive regulation of T cell proliferation GO:0042102 IMP
    positive regulation of T cell proliferation GO:0042102 ISO
    positive regulation of T cell proliferation GO:0042102 ISS
    T cell activation GO:0042110 IBA
    T cell activation GO:0042110 ISO
    phosphatidylinositol 3-kinase/protein kinase B signal transduction GO:0043491 IDA
    negative thymic T cell selection GO:0045060 IMP
    regulatory T cell differentiation GO:0045066 IEA
    regulatory T cell differentiation GO:0045066 ISO
    regulation of regulatory T cell differentiation GO:0045589 IGI
    positive regulation of mitotic nuclear division GO:0045840 ISO
    positive regulation of mitotic nuclear division GO:0045840 ISS
    positive regulation of transcription by RNA polymerase II GO:0045944 IGI
    positive regulation of isotype switching to IgG isotypes GO:0048304 IMP
    T cell receptor signaling pathway GO:0050852 IBA
    T cell receptor signaling pathway GO:0050852 IMP
    positive regulation of phosphatidylinositol 3-kinase/protein kinase B signal transduction GO:0051897 IDA
    apoptotic signaling pathway GO:0097190 IDA
    positive regulation of CD4-positive, alpha-beta T cell proliferation GO:2000563 IDA
    positive regulation of CD4-positive, alpha-beta T cell proliferation GO:2000563 IMP
Subcellular Localization
    immunological synapse GO:0001772 IDA
    plasma membrane GO:0005886 ISO
    external side of plasma membrane GO:0009897 IBA
    external side of plasma membrane GO:0009897 IDA
    external side of plasma membrane GO:0009897 IMP
    external side of plasma membrane GO:0009897 ISO
    cell surface GO:0009986 ISO
    protein complex involved in cell adhesion GO:0098636 ISO
 Experiment description of studies that identified Cd28 in sEVs
1
Experiment ID 907
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 908
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Cd28
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.



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