Gene description for Cd28
Gene name CD28 antigen
Gene symbol Cd28
Other names/aliases -
Species Mus musculus
 Database cross references - Cd28
ExoCarta ExoCarta_12487
Vesiclepedia VP_12487
Entrez Gene 12487
UniProt P31041  
 Cd28 identified in exosomes derived from the following tissue/cell type
Colon cancer cells 37309723    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Cd28
Molecular Function
    protein homodimerization activity GO:0042803 ISO
    protease binding GO:0002020 ISO
    SH3/SH2 adaptor activity GO:0005070 ISO
Biological Process
    immune response GO:0006955 IEA
    positive regulation of T cell proliferation GO:0042102 ISO
    positive regulation of inflammatory response to antigenic stimulus GO:0002863 IMP
    regulatory T cell differentiation GO:0045066 ISO
    positive regulation of phosphatidylinositol 3-kinase signaling GO:0014068 IDA
    positive regulation of interleukin-2 biosynthetic process GO:0045086 ISO
    positive regulation of signal transduction GO:0009967 ISO
    positive regulation of transcription from RNA polymerase II promoter GO:0045944 IGI
    positive regulation of alpha-beta T cell proliferation GO:0046641 IMP
    positive regulation of mitotic nuclear division GO:0045840 ISO
    positive regulation of isotype switching to IgG isotypes GO:0048304 IMP
    T cell activation GO:0042110 ISO
    positive regulation of protein kinase B signaling GO:0051897 IDA
    apoptotic signaling pathway GO:0097190 IDA
    T cell receptor signaling pathway GO:0050852 IMP
    negative thymic T cell selection GO:0045060 IMP
    T cell costimulation GO:0031295 ISO
Subcellular Localization
    immunological synapse GO:0001772 IDA
    membrane GO:0016020 IEA
    plasma membrane GO:0005886 ISO
    integral component of membrane GO:0016021 IEA
    external side of plasma membrane GO:0009897 ISO
    membrane raft GO:0045121 ISO
 Experiment description of studies that identified Cd28 in exosomes
1
Experiment ID 907
MISEV standards
✔
EM
EV Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
EV Enriched markers
✔
HSPA5
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S.
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 908
MISEV standards
✔
EM
EV Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
EV Enriched markers
✔
HSPA5
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S.
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Cd28
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.



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