Gene description for SPNS2
Gene name spinster homolog 2 (Drosophila)
Gene symbol SPNS2
Other names/aliases -
Species Homo sapiens
 Database cross references - SPNS2
ExoCarta ExoCarta_124976
Vesiclepedia VP_124976
Entrez Gene 124976
HGNC 26992
MIM 612584
UniProt Q8IVW8  
 SPNS2 identified in sEVs derived from the following tissue/cell type
Retinal pigment epithelial cells 35333565    
Retinal pigment epithelial cells 35333565    
 Gene ontology annotations for SPNS2
Molecular Function
    transmembrane transporter activity GO:0022857 IBA
    sphingolipid transporter activity GO:0046624 IBA
    sphingolipid transporter activity GO:0046624 IDA
    sphingolipid transporter activity GO:0046624 TAS
Biological Process
    B cell homeostasis GO:0001782 IEA
    regulation of humoral immune response GO:0002920 ISS
    sphingosine-1-phosphate receptor signaling pathway GO:0003376 IBA
    sphingosine-1-phosphate receptor signaling pathway GO:0003376 ISS
    lipid transport GO:0006869 IBA
    lipid transport GO:0006869 IDA
    sensory perception of sound GO:0007605 ISS
    sphingolipid biosynthetic process GO:0030148 TAS
    T cell homeostasis GO:0043029 IEA
    regulation of eye pigmentation GO:0048073 IEA
    lymph node development GO:0048535 IEA
    transmembrane transport GO:0055085 IEA
    bone development GO:0060348 IEA
    lymphocyte migration GO:0072676 IEA
    regulation of T cell migration GO:2000404 ISS
Subcellular Localization
    plasma membrane GO:0005886 TAS
    endosome membrane GO:0010008 IEA
    membrane GO:0016020 IBA
 Experiment description of studies that identified SPNS2 in sEVs
1
Experiment ID 834
MISEV standards
✔
EM
Biophysical techniques
✔
LAMP2|CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 835
MISEV standards
✔
EM
Biophysical techniques
✔
CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for SPNS2
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 HNRNPH1 3187
Affinity Capture-RNA Homo sapiens
2 KRAS 3845
Synthetic Lethality Homo sapiens
View the network image/svg+xml
 Pathways in which SPNS2 is involved
PathwayEvidenceSource
Metabolism TAS Reactome
Metabolism of lipids TAS Reactome
Sphingolipid de novo biosynthesis TAS Reactome
Sphingolipid metabolism TAS Reactome





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