Gene description for Cfl1
Gene name cofilin 1, non-muscle
Gene symbol Cfl1
Other names/aliases AA959946
Cof
n-cofilin
Species Mus musculus
 Database cross references - Cfl1
ExoCarta ExoCarta_12631
Entrez Gene 12631
UniProt P18760  
 Cfl1 identified in exosomes derived from the following tissue/cell type
Dendritic cells 11390481    
Embryonic fibroblasts 18494037    
Fibroblasts 23260141    
Mast cells 17486113    
Microglia 16081791    
Mov neuroglial cells 15210972    
Oligodendrocytes 21136642    
 Gene ontology annotations for Cfl1
Molecular Function
    actin binding GO:0003779 ISO
Biological Process
    mitotic cytokinesis GO:0000281 IMP
    actin filament depolymerization GO:0030042 IEA
    neural fold formation GO:0001842 IMP
    positive regulation of actin filament depolymerization GO:0030836 IMP
    regulation of cell morphogenesis GO:0022604 ISO
    movement of cell or subcellular component GO:0006928 IMP
    protein phosphorylation GO:0006468 IDA
    cell projection organization GO:0030030 ISO
    actin filament organization GO:0007015 IMP
    cytoskeleton organization GO:0007010 ISO
    response to amino acid GO:0043200 IDA
    protein import into nucleus GO:0006606 ISO
    establishment of cell polarity GO:0030010 IMP
    neural crest cell migration GO:0001755 IMP
    negative regulation of cell size GO:0045792 ISO
    regulation of dendritic spine morphogenesis GO:0061001 ISO
Subcellular Localization
    plasma membrane GO:0005886 IEA
    cytoskeleton GO:0005856 IEA
    focal adhesion GO:0005925 ISO
    cortical actin cytoskeleton GO:0030864 IDA
    cell projection GO:0042995 IEA
    extracellular space GO:0005615 ISO
    lamellipodium GO:0030027 ISO
    cell leading edge GO:0031252 ISO
    intracellular GO:0005622 IEA
    extracellular exosome GO:0070062 ISO
    nucleus GO:0005634 IEA
    vesicle GO:0031982 ISO
    actin cytoskeleton GO:0015629 IEA
    cell-cell junction GO:0005911 IDA
    membrane GO:0016020 ISO
    cytoplasm GO:0005737 ISO
 Experiment description of studies that identified Cfl1 in exosomes
1
Experiment ID 10
ISEV standards
✔
EM
EV Biophysical techniques
✔
Alix
EV Cytosolic markers
✔
CD9|MHCII|MHCI|CD86|LAMP2
EV Membrane markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry [MALDI TOF]
PubMed ID 11390481    
Organism Mus musculus
Experiment description Proteomic analysis of dendritic cell-derived exosomes: a secreted subcellular compartment distinct from apoptotic vesicles.
Authors Thery C, Boussac M, Veron P, Ricciardi-Castagnoli P, Raposo G, Garin J, Amigorena S.
Journal name JIMMU
Publication year 2001
Sample Dendritic cells
Sample name D1
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Flotation density 1.15-1.18 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [MALDI TOF]
Mass spectrometry [QTOF]
Western blotting
2
Experiment ID 73
ISEV standards
✔
EM
EV Biophysical techniques
✔
HSP70|HSP90
EV Cytosolic markers
✘ EV Membrane markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 18494037    
Organism Mus musculus
Experiment description Difference gel electrophoresis analysis of Ras-transformed fibroblast cell-derived exosomes.
Authors Ji H, Erfani N, Tauro BJ, Kapp EA, Zhu HJ, Moritz RL, Lim JW, Simpson RJ
Journal name ELEC
Publication year 2008
Sample Embryonic fibroblasts
Sample name NIH3T3
Isolation/purification methods Differential centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [LCQ DECA]
Western blotting
3
Experiment ID 210
ISEV standards
✔
EM
EV Biophysical techniques
✘ EV Cytosolic markers
✔
CD81|FLOT1
EV Membrane markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23260141    
Organism Mus musculus
Experiment description Exosomes Mediate Stromal Mobilization of Autocrine Wnt-PCP Signaling in Breast Cancer Cell Migration.
Authors Luga V, Zhang L, Viloria-Petit AM, Ogunjimi AA, Inanlou MR, Chiu E, Buchanan M, Hosein AN, Basik M, Wrana JL.
Journal name Cell
Publication year 2012
Sample Fibroblasts
Sample name Normal-Fibroblasts (L cells)
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
4
Experiment ID 15
ISEV standards
✔
EM
EV Biophysical techniques
✘ EV Cytosolic markers
✔
CD63
EV Membrane markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 17486113    
Organism Mus musculus
Homo sapiens
Experiment description Exosome-mediated transfer of mRNAs and microRNAs is a novel mechanism of genetic exchange between cells.
Authors Valadi H, Ekstrom K, Bossios A, Sjostrand M, Lee JJ, Lotvall JO
Journal name NCB
Publication year 2007
Sample Mast cells
Sample name MC9
Bone marrow-derived mast cells
HMC-1
Isolation/purification methods Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.11-1.21 g/mL
Molecules identified in the study Protein
mRNA
miRNA
Methods used in the study Mass spectrometry [MALDI TOF]
Western blotting
Microarray
miRCURY LNA Array
5
Experiment ID 14
ISEV standards
✔
EM
EV Biophysical techniques
✔
RAB7|RAB11
EV Cytosolic markers
✔
CD9|CD63|LAMP1|LAMP2
EV Membrane markers
✔
DNM
EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 16081791    
Organism Mus musculus
Experiment description Proteomic analysis of microglia-derived exosomes: metabolic role of the aminopeptidase CD13 in neuropeptide catabolism.
Authors Potolicchio I, Carven GJ, Xu X, Stipp C, Riese RJ, Stern LJ, Santambrogio L
Journal name JIMMU
Publication year 2005
Sample Microglia
Sample name N9
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Flotation density 1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [LCQ DECA XP]
Western blotting
6
Experiment ID 39
ISEV standards
✔
IEM
EV Biophysical techniques
✔
TSG101|HSC70
EV Cytosolic markers
✔
FLOT1
EV Membrane markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 15210972    
Organism Mus musculus
Experiment description Cells release prions in association with exosomes.
Authors Fevrier B, Vilette D, Archer F, Loew D, Faigle W, Vidal M, Laude H, Raposo G
Journal name PNAS
Publication year 2004
Sample Mov neuroglial cells
Sample name Mov neuroglial cell
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Flotation density 1.14 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [QSTAR]
Western blotting
Immunoelectron Microscopy
7
Experiment ID 33
ISEV standards
✔
EM|IEM
EV Biophysical techniques
✔
Alix|TSG101
EV Cytosolic markers
✘ EV Membrane markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 21136642    
Organism Mus musculus
Experiment description Oligodendrocytes secrete exosomes containing major myelin and stress-protective proteins: Trophic support for axons?
Authors Kramer-Albers EM, Bretz N, Tenzer S, Winterstein C, Mobius W, Berger H, Nave KA, Schild H, Trotter J
Journal name PROTEOMICS_CL
Publication year 2007
Sample Oligodendrocytes
Sample name Oligodendrocytes
Oli-neu
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Flotation density 1.10-1.14 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [QTOF]
Western blotting
Immunoelectron Microscopy
 Protein-protein interactions for Cfl1
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.



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