Experiment description of studies that identified Cfl1 in sEVs

1 Experiment ID 907 MISEV standards ✔ EM Biophysical techniques ✔ Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a Enriched markers ✔ HSPA5 Negative markers ✔ NTA Particle analysis Identified molecule Protein Identification method Mass spectrometry PubMed ID 37309723     Organism Mus musculus Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S." Journal name Proteomics Publication year 2023 Sample Colon cancer cells Sample name C26 Isolation/purification methods Differential centrifugation Ultracentrifugation Flotation density - Molecules identified in the study Protein Methods used in the study Western blotting Mass spectrometry

2 Experiment ID 10 MISEV standards ✔ EM Biophysical techniques ✔ Alix|CD9|MHCII|MHCI|CD86|LAMP2 Enriched markers ✘ Negative markers ✘ Particle analysis Identified molecule protein Identification method Mass spectrometry [MALDI TOF] PubMed ID 11390481     Organism Mus musculus Experiment description Proteomic analysis of dendritic cell-derived exosomes: a secreted subcellular compartment distinct from apoptotic vesicles. Authors "Thery C, Boussac M, Veron P, Ricciardi-Castagnoli P, Raposo G, Garin J, Amigorena S." Journal name JIMMU Publication year 2001 Sample Dendritic cells Sample name D1 Isolation/purification methods Differential centrifugation Sucrose density gradient Flotation density 1.15-1.18 g/mL Molecules identified in the study Protein Methods used in the study Mass spectrometry [MALDI TOF] Mass spectrometry [QTOF] Western blotting

3 Experiment ID 73 MISEV standards ✔ EM Biophysical techniques ✔ HSP70|HSP90 Enriched markers ✘ Negative markers ✘ Particle analysis Identified molecule protein Identification method Mass spectrometry PubMed ID 18494037     Organism Mus musculus Experiment description Difference gel electrophoresis analysis of Ras-transformed fibroblast cell-derived exosomes. Authors "Ji H, Erfani N, Tauro BJ, Kapp EA, Zhu HJ, Moritz RL, Lim JW, Simpson RJ" Journal name ELEC Publication year 2008 Sample Embryonic fibroblasts Sample name NIH3T3 Isolation/purification methods Differential centrifugation Flotation density - Molecules identified in the study Protein Methods used in the study Mass spectrometry [LCQ DECA] Western blotting

4 Experiment ID 210 MISEV standards ✔ EM Biophysical techniques ✔ CD81|FLOT1 Enriched markers ✘ Negative markers ✘ Particle analysis Identified molecule protein Identification method Mass spectrometry PubMed ID 23260141     Organism Mus musculus Experiment description Exosomes Mediate Stromal Mobilization of Autocrine Wnt-PCP Signaling in Breast Cancer Cell Migration. Authors "Luga V, Zhang L, Viloria-Petit AM, Ogunjimi AA, Inanlou MR, Chiu E, Buchanan M, Hosein AN, Basik M, Wrana JL." Journal name Cell Publication year 2012 Sample Fibroblasts Sample name Normal-Fibroblasts (L cells) Isolation/purification methods Differential centrifugation Ultracentrifugation Flotation density - Molecules identified in the study Protein Methods used in the study Mass spectrometry

5 Experiment ID 15 MISEV standards ✔ EM Biophysical techniques ✔ CD63 Enriched markers ✘ Negative markers ✘ Particle analysis Identified molecule protein Identification method Mass spectrometry PubMed ID 17486113     Organism Mus musculus Homo sapiens Experiment description Exosome-mediated transfer of mRNAs and microRNAs is a novel mechanism of genetic exchange between cells. Authors "Valadi H, Ekstrom K, Bossios A, Sjostrand M, Lee JJ, Lotvall JO" Journal name NCB Publication year 2007 Sample Mast cells Sample name MC9 Bone marrow-derived mast cells HMC-1 Isolation/purification methods Filtration Ultracentrifugation Sucrose density gradient Flotation density 1.11-1.21 g/mL Molecules identified in the study Protein mRNA miRNA Methods used in the study Mass spectrometry [MALDI TOF] Western blotting Microarray miRCURY LNA Array

6 Experiment ID 14 MISEV standards ✔ EM Biophysical techniques ✔ RAB7|RAB11|CD9|CD63|LAMP1|LAMP2 Enriched markers ✔ DNM Negative markers ✘ Particle analysis Identified molecule protein Identification method Mass spectrometry PubMed ID 16081791     Organism Mus musculus Experiment description Proteomic analysis of microglia-derived exosomes: metabolic role of the aminopeptidase CD13 in neuropeptide catabolism. Authors "Potolicchio I, Carven GJ, Xu X, Stipp C, Riese RJ, Stern LJ, Santambrogio L" Journal name JIMMU Publication year 2005 Sample Microglia Sample name N9 Isolation/purification methods Differential centrifugation Sucrose density gradient Flotation density 1.15 g/mL Molecules identified in the study Protein Methods used in the study Mass spectrometry [LCQ DECA XP] Western blotting

7 Experiment ID 39 MISEV standards ✔ IEM Biophysical techniques ✔ TSG101|HSC70|FLOT1 Enriched markers ✘ Negative markers ✘ Particle analysis Identified molecule protein Identification method Mass spectrometry PubMed ID 15210972     Organism Mus musculus Experiment description Cells release prions in association with exosomes. Authors "Fevrier B, Vilette D, Archer F, Loew D, Faigle W, Vidal M, Laude H, Raposo G" Journal name PNAS Publication year 2004 Sample Mov neuroglial cells Sample name Mov neuroglial cell Isolation/purification methods Differential centrifugation Sucrose density gradient Flotation density 1.14 g/mL Molecules identified in the study Protein Methods used in the study Mass spectrometry [QSTAR] Western blotting Immunoelectron Microscopy

8 Experiment ID 33 MISEV standards ✔ EM|IEM Biophysical techniques ✔ Alix|TSG101 Enriched markers ✘ Negative markers ✘ Particle analysis Identified molecule protein Identification method Mass spectrometry PubMed ID 21136642     Organism Mus musculus Experiment description Oligodendrocytes secrete exosomes containing major myelin and stress-protective proteins: Trophic support for axons? Authors "Kramer-Albers EM, Bretz N, Tenzer S, Winterstein C, Mobius W, Berger H, Nave KA, Schild H, Trotter J" Journal name PROTEOMICS_CL Publication year 2007 Sample Oligodendrocytes Sample name Oligodendrocytes Oli-neu Isolation/purification methods Differential centrifugation Sucrose density gradient Flotation density 1.10-1.14 g/mL Molecules identified in the study Protein Methods used in the study Mass spectrometry [QTOF] Western blotting Immunoelectron Microscopy

9 Experiment ID 908 MISEV standards ✔ EM Biophysical techniques ✔ Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a Enriched markers ✔ HSPA5 Negative markers ✔ NTA Particle analysis Identified molecule Protein Identification method Mass spectrometry PubMed ID 37309723     Organism Mus musculus Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S." Journal name Proteomics Publication year 2023 Sample T-cell lymphoma cells Sample name EL4 Isolation/purification methods Differential centrifugation Ultracentrifugation Flotation density - Molecules identified in the study Protein Methods used in the study Western blotting Mass spectrometry

Protein-protein interactions for Cfl1

Protein Interactor ExoCarta ID Identification method PubMed Species 1 Ipo9   Affinity Capture-Western Mus musculus 2 Fancd2   Affinity Capture-MS Mus musculus 3 Tmem173   Proximity Label-MS Mus musculus 4 Mapt   Affinity Capture-MS Mus musculus 5 Eed   Affinity Capture-MS Mus musculus 6 MAPT   Reconstituted Complex Homo sapiens 7 Dlg4   Affinity Capture-MS Mus musculus 8 Tfe3   Affinity Capture-MS Mus musculus 9 SSH2   Biochemical Activity Homo sapiens 10 MTNR1A   Reconstituted Complex Homo sapiens Co-purification Homo sapiens 11 LIMK1   Biochemical Activity Drosophila melanogaster 12 SSH1   Biochemical Activity Homo sapiens 13 Kctd13   Affinity Capture-MS Mus musculus 14 MTNR1B   Reconstituted Complex Homo sapiens 15 Kcnma1   Affinity Capture-MS Mus musculus Affinity Capture-Western Mus musculus View the network

Pathways in which Cfl1 is involved

No pathways found