Gene description for Col12a1
Gene name collagen, type XII, alpha 1
Gene symbol Col12a1
Other names/aliases -
Species Mus musculus
 Database cross references - Col12a1
ExoCarta ExoCarta_12816
Vesiclepedia VP_12816
Entrez Gene 12816
UniProt Q60847  
 Col12a1 identified in sEVs derived from the following tissue/cell type
Colon cancer cells 37309723    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Col12a1
Molecular Function
    extracellular matrix structural constituent conferring tensile strength GO:0030020 RCA
Biological Process
    cell adhesion GO:0007155 IEA
    endodermal cell differentiation GO:0035987 IBA
    endodermal cell differentiation GO:0035987 IEA
Subcellular Localization
    collagen trimer GO:0005581 IEA
    collagen type XII trimer GO:0005595 ISO
    extracellular space GO:0005615 HDA
    collagen-containing extracellular matrix GO:0062023 HDA
 Experiment description of studies that identified Col12a1 in sEVs
1
Experiment ID 907
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 908
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Col12a1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Dysf  
Co-fractionation Mus musculus
2 Mybpc2  
Co-fractionation Mus musculus
3 Col11a1  
Co-fractionation Mus musculus
View the network image/svg+xml



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