Gene description for Col4a1
Gene name collagen, type IV, alpha 1
Gene symbol Col4a1
Other names/aliases Bru
Col4a-1
Del(8)44H
Raw
Svc
Species Mus musculus
 Database cross references - Col4a1
ExoCarta ExoCarta_12826
Vesiclepedia VP_12826
Entrez Gene 12826
UniProt P02463  
 Col4a1 identified in sEVs derived from the following tissue/cell type
Colon cancer cells 37309723    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Col4a1
Molecular Function
    extracellular matrix structural constituent GO:0005201 ISO
    extracellular matrix structural constituent conferring tensile strength GO:0030020 IBA
    extracellular matrix structural constituent conferring tensile strength GO:0030020 ISO
    extracellular matrix structural constituent conferring tensile strength GO:0030020 RCA
    platelet-derived growth factor binding GO:0048407 ISO
Biological Process
    branching involved in blood vessel morphogenesis GO:0001569 IEA
    branching involved in blood vessel morphogenesis GO:0001569 ISO
    brain development GO:0007420 IEA
    brain development GO:0007420 ISO
    neuromuscular junction development GO:0007528 IMP
    extracellular matrix organization GO:0030198 IBA
    epithelial cell differentiation GO:0030855 IEA
    collagen-activated tyrosine kinase receptor signaling pathway GO:0038063 IGI
    blood vessel morphogenesis GO:0048514 ISO
    retinal blood vessel morphogenesis GO:0061304 IEA
    retinal blood vessel morphogenesis GO:0061304 ISO
    renal tubule morphogenesis GO:0061333 IEA
    renal tubule morphogenesis GO:0061333 ISO
    cellular response to amino acid stimulus GO:0071230 IDA
    basement membrane organization GO:0071711 IEA
    basement membrane organization GO:0071711 ISO
Subcellular Localization
    extracellular region GO:0005576 TAS
    collagen type IV trimer GO:0005587 IDA
    collagen type IV trimer GO:0005587 ISO
    basement membrane GO:0005604 IDA
    extracellular space GO:0005615 HDA
    extracellular space GO:0005615 IBA
    collagen-containing extracellular matrix GO:0062023 HDA
    collagen-containing extracellular matrix GO:0062023 IBA
 Experiment description of studies that identified Col4a1 in sEVs
1
Experiment ID 907
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 908
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Col4a1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Col2a1 12824
Co-fractionation Mus musculus
2 Eed  
Affinity Capture-MS Mus musculus
3 Bmp3  
Co-fractionation Mus musculus
View the network image/svg+xml



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