Gene description for Col6a1
Gene name collagen, type VI, alpha 1
Gene symbol Col6a1
Other names/aliases AI747156
Col6a-1
Species Mus musculus
 Database cross references - Col6a1
ExoCarta ExoCarta_12833
Vesiclepedia VP_12833
Entrez Gene 12833
UniProt Q04857  
 Col6a1 identified in sEVs derived from the following tissue/cell type
Colon cancer cells 37309723    
Embryonic fibroblasts 18494037    
Fibroblasts 23260141    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Col6a1
Molecular Function
    protein binding GO:0005515 IPI
    collagen binding GO:0005518 ISO
    extracellular matrix structural constituent conferring tensile strength GO:0030020 RCA
    platelet-derived growth factor binding GO:0048407 ISO
Biological Process
    response to reactive oxygen species GO:0000302 IMP
    cell morphogenesis GO:0000902 IMP
    ossification GO:0001503 IMP
    hair follicle development GO:0001942 IDA
    hair follicle development GO:0001942 IMP
    reduction of food intake in response to dietary excess GO:0002023 IMP
    muscle system process GO:0003012 IMP
    respiratory system process GO:0003016 IMP
    glycolytic process GO:0006096 IMP
    tricarboxylic acid cycle GO:0006099 IMP
    2-oxoglutarate metabolic process GO:0006103 IMP
    energy reserve metabolic process GO:0006112 IMP
    protein phosphorylation GO:0006468 IMP
    protein lipidation GO:0006497 IMP
    autophagy GO:0006914 IMP
    apoptotic process GO:0006915 IGI
    apoptotic process GO:0006915 IMP
    inflammatory response GO:0006954 IMP
    organelle organization GO:0006996 IMP
    mitochondrion organization GO:0007005 IGI
    mitochondrion organization GO:0007005 IMP
    cell adhesion GO:0007155 IEA
    single fertilization GO:0007338 IGI
    peripheral nervous system development GO:0007422 IMP
    heart development GO:0007507 IMP
    muscle organ development GO:0007517 IMP
    skeletal muscle tissue development GO:0007519 IMP
    circadian rhythm GO:0007623 IMP
    metabolic process GO:0008152 IMP
    cell death GO:0008219 IMP
    insulin receptor signaling pathway GO:0008286 IMP
    regulation of cell size GO:0008361 IMP
    response to xenobiotic stimulus GO:0009410 IMP
    response to UV GO:0009411 IDA
    response to wounding GO:0009611 IDA
    response to wounding GO:0009611 IMP
    response to mechanical stimulus GO:0009612 IMP
    response to toxic substance GO:0009636 IMP
    gene expression GO:0010467 IMP
    muscle cell apoptotic process GO:0010657 IMP
    response to activity GO:0014823 IMP
    response to muscle activity GO:0014850 IMP
    transmission of nerve impulse GO:0019226 IMP
    myelination in peripheral nervous system GO:0022011 IMP
    collagen fibril organization GO:0030199 IGI
    collagen fibril organization GO:0030199 IMP
    apoptotic nuclear changes GO:0030262 IMP
    bone mineralization GO:0030282 IMP
    lung development GO:0030324 IMP
    response to lipopolysaccharide GO:0032496 IMP
    collagen metabolic process GO:0032963 IMP
    response to lipid GO:0033993 IMP
    endodermal cell differentiation GO:0035987 IEA
    limb joint morphogenesis GO:0036022 IMP
    response to decreased oxygen levels GO:0036293 IMP
    skeletal muscle tissue regeneration GO:0043403 IMP
    phosphatidylinositol 3-kinase/protein kinase B signal transduction GO:0043491 IDA
    phosphatidylinositol 3-kinase/protein kinase B signal transduction GO:0043491 IMP
    skin development GO:0043588 IMP
    insulin-like growth factor receptor signaling pathway GO:0048009 IMP
    response to pain GO:0048265 IMP
    lung alveolus development GO:0048286 IMP
    skeletal muscle tissue growth GO:0048630 IMP
    skeletal muscle fiber development GO:0048741 IMP
    tissue remodeling GO:0048771 IMP
    homeostasis of number of cells GO:0048872 IMP
    nervous system process GO:0050877 IMP
    sensory perception of mechanical stimulus GO:0050954 IMP
    cartilage development GO:0051216 IMP
    protein tetramerization GO:0051262 IDA
    neuron apoptotic process GO:0051402 IDA
    mitochondrial depolarization GO:0051882 IGI
    mitochondrial depolarization GO:0051882 IMP
    muscle cell development GO:0055001 IMP
    uterus development GO:0060065 IGI
    canonical Wnt signaling pathway GO:0060070 IMP
    bone development GO:0060348 IMP
    lung morphogenesis GO:0060425 IMP
    lung epithelial cell differentiation GO:0060487 IMP
    skeletal muscle organ development GO:0060538 IMP
    adipose tissue development GO:0060612 IMP
    muscle structure development GO:0061061 IGI
    fat cell proliferation GO:0070341 IMP
    caveola assembly GO:0070836 IMP
    cellular response to amino acid stimulus GO:0071230 IDA
    basement membrane organization GO:0071711 IMP
    multicellular organismal locomotion GO:0071965 IMP
    reactive oxygen species metabolic process GO:0072593 IMP
    extracellular matrix assembly GO:0085029 IMP
    energy homeostasis GO:0097009 IMP
    skeletal muscle fiber differentiation GO:0098528 IGI
    response to peptide GO:1901652 IMP
    regulation of collagen fibril organization GO:1904026 IMP
    response to polyamine macromolecule GO:1904583 IMP
    response to bleomycin GO:1904975 IMP
    mitochondrial transmembrane transport GO:1990542 IMP
Subcellular Localization
    extracellular region GO:0005576 ISO
    collagen trimer GO:0005581 IEA
    basement membrane GO:0005604 IDA
    extracellular space GO:0005615 HDA
    mitochondrion GO:0005739 IEA
    lysosome GO:0005764 IDA
    sarcoplasmic reticulum GO:0016529 IMP
    myofibril GO:0030016 IMP
    extracellular matrix GO:0031012 IDA
    extracellular matrix GO:0031012 IDA
    extracellular matrix GO:0031012 IMP
    protein-containing complex GO:0032991 ISO
    sarcolemma GO:0042383 IDA
    collagen-containing extracellular matrix GO:0062023 HDA
    collagen-containing extracellular matrix GO:0062023 IBA
    intracellular vesicle GO:0097708 IDA
 Experiment description of studies that identified Col6a1 in sEVs
1
Experiment ID 907
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 73
MISEV standards
✔
EM
Biophysical techniques
✔
HSP70|HSP90
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 18494037    
Organism Mus musculus
Experiment description Difference gel electrophoresis analysis of Ras-transformed fibroblast cell-derived exosomes.
Authors "Ji H, Erfani N, Tauro BJ, Kapp EA, Zhu HJ, Moritz RL, Lim JW, Simpson RJ"
Journal name ELEC
Publication year 2008
Sample Embryonic fibroblasts
Sample name NIH3T3
Isolation/purification methods Differential centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [LCQ DECA]
Western blotting
3
Experiment ID 210
MISEV standards
✔
EM
Biophysical techniques
✔
CD81|FLOT1
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23260141    
Organism Mus musculus
Experiment description Exosomes Mediate Stromal Mobilization of Autocrine Wnt-PCP Signaling in Breast Cancer Cell Migration.
Authors "Luga V, Zhang L, Viloria-Petit AM, Ogunjimi AA, Inanlou MR, Chiu E, Buchanan M, Hosein AN, Basik M, Wrana JL."
Journal name Cell
Publication year 2012
Sample Fibroblasts
Sample name Normal-Fibroblasts (L cells)
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
4
Experiment ID 908
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Col6a1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Ckmt2  
Cross-Linking-MS (XL-MS) Mus musculus
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