Gene description for Dag1
Gene name dystroglycan 1
Gene symbol Dag1
Other names/aliases D9Wsu13e
DG
Dp427
Dp71
Species Mus musculus
 Database cross references - Dag1
ExoCarta ExoCarta_13138
Vesiclepedia VP_13138
Entrez Gene 13138
UniProt Q62165  
 Dag1 identified in exosomes derived from the following tissue/cell type
Colon cancer cells 37309723    
Fibroblasts 23260141    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Dag1
Molecular Function
    alpha-actinin binding GO:0051393 ISO
    protein complex binding GO:0032403 ISO
    structural constituent of muscle GO:0008307 ISO
    SH2 domain binding GO:0042169 ISO
    tubulin binding GO:0015631 ISO
    protein binding GO:0005515 IPI
    actin binding GO:0003779 ISO
    calcium ion binding GO:0005509 IEA
    vinculin binding GO:0017166 ISO
    dystroglycan binding GO:0002162 ISO
Biological Process
    Schwann cell development GO:0014044 IMP
    nerve maturation GO:0021682 IMP
    commissural neuron axon guidance GO:0071679 IMP
    branching involved in salivary gland morphogenesis GO:0060445 IMP
    calcium-dependent cell-matrix adhesion GO:0016340 ISO
    membrane protein ectodomain proteolysis GO:0006509 ISO
    modulation by virus of host morphology or physiology GO:0019048 ISO
    basement membrane organization GO:0071711 IMP
    microtubule anchoring GO:0034453 ISO
    negative regulation of MAPK cascade GO:0043409 ISO
    negative regulation of protein kinase B signaling GO:0051898 ISO
    myelination in peripheral nervous system GO:0022011 IMP
    NLS-bearing protein import into nucleus GO:0006607 ISO
    epithelial tube branching involved in lung morphogenesis GO:0060441 IMP
    negative regulation of cell migration GO:0030336 ISO
    morphogenesis of an epithelial sheet GO:0002011 IMP
    cytoskeletal anchoring at plasma membrane GO:0007016 ISO
    viral process GO:0016032 IEA
Subcellular Localization
    basement membrane GO:0005604 ISO
    focal adhesion GO:0005925 ISO
    sarcolemma GO:0042383 ISO
    membrane GO:0016020 IDA
    nucleoplasm GO:0005654 ISO
    extracellular space GO:0005615 ISO
    postsynaptic membrane GO:0045211 IEA
    cell-cell adherens junction GO:0005913 ISO
    nucleus GO:0005634 IEA
    dystroglycan complex GO:0016011 IDA
    membrane raft GO:0045121 IDA
    plasma membrane GO:0005886 ISO
    filopodium GO:0030175 ISO
    basolateral plasma membrane GO:0016323 ISO
    extracellular region GO:0005576 IEA
    dystrophin-associated glycoprotein complex GO:0016010 ISO
    integral component of membrane GO:0016021 ISO
    cytoplasm GO:0005737 ISO
    cytoskeleton GO:0005856 IEA
    contractile ring GO:0070938 ISO
    lamellipodium GO:0030027 ISO
    cell junction GO:0030054 IEA
    costamere GO:0043034 ISO
    synapse GO:0045202 IEA
    cell-cell junction GO:0005911 IDA
    extracellular exosome GO:0070062 ISO
    node of Ranvier GO:0033268 IMP
    cell outer membrane GO:0009279 IDA
 Experiment description of studies that identified Dag1 in exosomes
1
Experiment ID 907
MISEV standards
✔
EM
EV Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
EV Enriched markers
✔
HSPA5
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S.
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 210
MISEV standards
✔
EM
EV Biophysical techniques
✔
CD81|FLOT1
EV Enriched markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23260141    
Organism Mus musculus
Experiment description Exosomes Mediate Stromal Mobilization of Autocrine Wnt-PCP Signaling in Breast Cancer Cell Migration.
Authors Luga V, Zhang L, Viloria-Petit AM, Ogunjimi AA, Inanlou MR, Chiu E, Buchanan M, Hosein AN, Basik M, Wrana JL.
Journal name Cell
Publication year 2012
Sample Fibroblasts
Sample name Normal-Fibroblasts (L cells)
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
3
Experiment ID 908
MISEV standards
✔
EM
EV Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
EV Enriched markers
✔
HSPA5
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S.
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Dag1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Sgcd  
Affinity Capture-Western Mus musculus
View the network image/svg+xml
 Pathways in which Dag1 is involved
PathwayEvidenceSource
ECM proteoglycans IEA Reactome
Integrin cell surface interactions IEA Reactome
Non-integrin membrane-ECM interactions IEA Reactome





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