Gene description for Ddost
Gene name dolichyl-di-phosphooligosaccharide-protein glycotransferase
Gene symbol Ddost
Other names/aliases -
Species Mus musculus
 Database cross references - Ddost
ExoCarta ExoCarta_13200
Entrez Gene 13200
UniProt O54734  
 Ddost identified in exosomes derived from the following tissue/cell type
Mast cells 17486113    
 Gene ontology annotations for Ddost
Molecular Function
    dolichyl-diphosphooligosaccharide-protein glycotransferase activity GO:0004579 ISO
    transferase activity GO:0016740 IEA
    transferase activity, transferring glycosyl groups GO:0016757 IEA
Biological Process
    protein N-linked glycosylation via asparagine GO:0018279 IEA
    protein N-linked glycosylation GO:0006487 ISO
    T cell activation GO:0042110 ISO
    response to cytokine GO:0034097 ISO
Subcellular Localization
    membrane GO:0016020 ISO
    integral component of membrane GO:0016021 IEA
    endoplasmic reticulum GO:0005783 ISO
    intracellular membrane-bounded organelle GO:0043231 ISO
    endoplasmic reticulum membrane GO:0005789 IEA
    protein complex GO:0043234 ISO
 Experiment description of studies that identified Ddost in exosomes
1
Experiment ID 15
ISEV standards
EM
EV Biophysical techniques
EV Cytosolic markers
CD63
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule mrna
Identification method Microarray
PubMed ID 17486113    
Organism Mus musculus
Homo sapiens
Experiment description Exosome-mediated transfer of mRNAs and microRNAs is a novel mechanism of genetic exchange between cells.
Authors Valadi H, Ekstrom K, Bossios A, Sjostrand M, Lee JJ, Lotvall JO
Journal name NCB
Publication year 2007
Sample Mast cells
Sample name MC9
Bone marrow-derived mast cells
HMC-1
Isolation/purification methods Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.11-1.21 g/mL
Molecules identified in the study Protein
mRNA
miRNA
Methods used in the study Mass spectrometry [MALDI TOF]
Western blotting
Microarray
miRCURY LNA Array
 Protein-protein interactions for Ddost
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.



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