Gene description for ADM
Gene name adrenomedullin
Gene symbol ADM
Other names/aliases AM
PAMP
Species Homo sapiens
 Database cross references - ADM
ExoCarta ExoCarta_133
Vesiclepedia VP_133
Entrez Gene 133
HGNC 259
MIM 103275
UniProt P35318  
 ADM identified in exosomes derived from the following tissue/cell type
Hepatocellular carcinoma cells 26054723    
Pancreatic cancer cells 26061593    
Pancreatic cancer cells 26061593    
Pancreatic cancer cells 26061593    
Pancreatic cancer cells 26061593    
Plasma 26061593    
Plasma 26061593    
 Gene ontology annotations for ADM
Molecular Function
    hormone activity GO:0005179 IEA
    adrenomedullin receptor binding GO:0031700 IEA
    receptor binding GO:0005102 TAS
    protein binding GO:0005515 IPI
Biological Process
    response to hypoxia GO:0001666 IEA
    positive regulation of vasculogenesis GO:2001214 IEA
    cell-cell signaling GO:0007267 TAS
    response to wounding GO:0009611 IEA
    neuron projection regeneration GO:0031102 IEA
    blood circulation GO:0008015 TAS
    androgen metabolic process GO:0008209 IEA
    response to insulin GO:0032868 IEA
    heart development GO:0007507 IEA
    developmental growth GO:0048589 IEA
    positive regulation of cell proliferation GO:0008284 IEA
    vasculogenesis GO:0001570 IDA
    positive regulation of vasodilation GO:0045909 IEA
    female pregnancy GO:0007565 IEA
    response to lipopolysaccharide GO:0032496 IEA
    spongiotrophoblast layer development GO:0060712 IEA
    aging GO:0007568 IEA
    signal transduction GO:0007165 TAS
    negative regulation of vasoconstriction GO:0045906 IDA
    odontogenesis of dentin-containing tooth GO:0042475 IEA
    negative regulation of vascular permeability GO:0043116 IEA
    G-protein coupled receptor internalization GO:0002031 IDA
    positive regulation of angiogenesis GO:0045766 IEA
    vascular smooth muscle cell development GO:0097084 IEA
    hormone secretion GO:0046879 IEA
    receptor internalization GO:0031623 IDA
    branching involved in labyrinthine layer morphogenesis GO:0060670 IEA
    organ regeneration GO:0031100 IEA
    positive regulation of apoptotic process GO:0043065 IEA
    response to starvation GO:0042594 IEA
    cAMP biosynthetic process GO:0006171 IDA
    positive regulation of cytosolic calcium ion concentration GO:0007204 IEA
    cAMP-mediated signaling GO:0019933 IEA
    regulation of the force of heart contraction GO:0002026 IEA
    progesterone biosynthetic process GO:0006701 TAS
    neural tube closure GO:0001843 IEA
    positive regulation of heart rate GO:0010460 IEA
    negative regulation of cell proliferation GO:0008285 IEA
    response to glucocorticoid GO:0051384 IEA
    response to cold GO:0009409 IEA
    positive regulation of cAMP biosynthetic process GO:0030819 IDA
Subcellular Localization
    cytosol GO:0005829 IEA
    cytoplasm GO:0005737 IDA
    extracellular space GO:0005615 IDA
    extracellular region GO:0005576 TAS
 Experiment description of studies that identified ADM in exosomes
1
Experiment ID 235
MISEV standards
EM
EV Biophysical techniques
TSG101|Alix|HSC70|GAPDH
EV Enriched markers
HSP90B1
EV Negative markers
qNano
EV Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocellular carcinoma cells
Sample name HKCI-8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RT-PCR
RNA Sequencing
2
Experiment ID 951
MISEV standards
EV Biophysical techniques
EV Enriched markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 26061593    
Organism Homo sapiens
Experiment description Pathogenesis of pancreatic cancer exosome-induced lipolysis in adipose tissue
Authors Sagar G, Sah RP, Javeed N, Dutta SK, Smyrk TC, Lau JS, Giorgadze N, Tchkonia T, Kirkland JL, Chari ST, Mukhopadhyay D.
Journal name Gut
Publication year 2017
Sample Pancreatic cancer cells
Sample name PANC-1 - Adrenomedullin immunoprecipitation
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
3
Experiment ID 952
MISEV standards
EV Biophysical techniques
EV Enriched markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 26061593    
Organism Homo sapiens
Experiment description Pathogenesis of pancreatic cancer exosome-induced lipolysis in adipose tissue
Authors Sagar G, Sah RP, Javeed N, Dutta SK, Smyrk TC, Lau JS, Giorgadze N, Tchkonia T, Kirkland JL, Chari ST, Mukhopadhyay D.
Journal name Gut
Publication year 2017
Sample Pancreatic cancer cells
Sample name PANC-1 - Adrenomedullin immunoprecipitation
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
4
Experiment ID 953
MISEV standards
EV Biophysical techniques
EV Enriched markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 26061593    
Organism Homo sapiens
Experiment description Pathogenesis of pancreatic cancer exosome-induced lipolysis in adipose tissue
Authors Sagar G, Sah RP, Javeed N, Dutta SK, Smyrk TC, Lau JS, Giorgadze N, Tchkonia T, Kirkland JL, Chari ST, Mukhopadhyay D.
Journal name Gut
Publication year 2017
Sample Pancreatic cancer cells
Sample name 6741-1 (MCPAN014) - Adrenomedullin immunoprecipitation
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
5
Experiment ID 954
MISEV standards
EV Biophysical techniques
EV Enriched markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 26061593    
Organism Homo sapiens
Experiment description Pathogenesis of pancreatic cancer exosome-induced lipolysis in adipose tissue
Authors Sagar G, Sah RP, Javeed N, Dutta SK, Smyrk TC, Lau JS, Giorgadze N, Tchkonia T, Kirkland JL, Chari ST, Mukhopadhyay D.
Journal name Gut
Publication year 2017
Sample Pancreatic cancer cells
Sample name 6741-1 (MCPAN014) - Adrenomedullin immunoprecipitation
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
6
Experiment ID 949
MISEV standards
EV Biophysical techniques
CD63|TSG101
EV Enriched markers
EV Negative markers
NTA
EV Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 26061593    
Organism Homo sapiens
Experiment description Pathogenesis of pancreatic cancer exosome-induced lipolysis in adipose tissue
Authors Sagar G, Sah RP, Javeed N, Dutta SK, Smyrk TC, Lau JS, Giorgadze N, Tchkonia T, Kirkland JL, Chari ST, Mukhopadhyay D.
Journal name Gut
Publication year 2017
Sample Plasma
Sample name Non-pancreatic cancer control
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
7
Experiment ID 950
MISEV standards
EV Biophysical techniques
CD63|TSG101
EV Enriched markers
EV Negative markers
NTA
EV Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 26061593    
Organism Homo sapiens
Experiment description Pathogenesis of pancreatic cancer exosome-induced lipolysis in adipose tissue
Authors Sagar G, Sah RP, Javeed N, Dutta SK, Smyrk TC, Lau JS, Giorgadze N, Tchkonia T, Kirkland JL, Chari ST, Mukhopadhyay D.
Journal name Gut
Publication year 2017
Sample Plasma
Sample name Pancreatic cancer patients
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
 Protein-protein interactions for ADM
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 ADMR  
Invivo Homo sapiens
2 CFH 3075
Invitro Homo sapiens
3 EDN1  
Invivo Homo sapiens
View the network image/svg+xml
 Pathways in which ADM is involved
PathwayEvidenceSource
Calcitonin-like ligand receptors TAS Reactome
G alpha (s) signalling events TAS Reactome





Perform bioinformatics analysis of your extracellular vesicle data set using FunRich, a open access standalone tool. NEW UPDATED VERSION OF FunRich available for download (12/09/2016) from here