Gene ontology annotations for ADM
Experiment description of studies that identified ADM in exosomes
1
Experiment ID
235
MISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101|Alix|HSC70|GAPDH
EV Enriched markers
✔
HSP90B1
EV Negative markers
✔
qNano
EV Particle analysis
Identified molecule
mRNA
Identification method
RNA Sequencing
PubMed ID
26054723
Organism
Homo sapiens
Experiment description
Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors
He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N
Journal name
Carcinogenesis
Publication year
2015
Sample
Hepatocellular carcinoma cells
Sample name
HKCI-8
Isolation/purification methods
Differential centrifugation Filtration Ultracentrifugation Sucrose density gradient
Flotation density
1.13-1.19 g/mL
Molecules identified in the study
Protein RNA
Methods used in the study
Western blotting Mass spectrometry RT-PCR RNA Sequencing
2
Experiment ID
951
MISEV standards
✘
EV Biophysical techniques
EV Enriched markers
✘
EV Negative markers
✘
EV Particle analysis
Identified molecule
protein
Identification method
Western blotting
PubMed ID
26061593
Organism
Homo sapiens
Experiment description
Pathogenesis of pancreatic cancer exosome-induced lipolysis in adipose tissue
Authors
Sagar G, Sah RP, Javeed N, Dutta SK, Smyrk TC, Lau JS, Giorgadze N, Tchkonia T, Kirkland JL, Chari ST, Mukhopadhyay D.
Journal name
Gut
Publication year
2017
Sample
Pancreatic cancer cells
Sample name
PANC-1 - Adrenomedullin immunoprecipitation
Isolation/purification methods
Differential centrifugation Ultracentrifugation
Flotation density
-
Molecules identified in the study
Protein
Methods used in the study
Western blotting
3
Experiment ID
952
MISEV standards
✘
EV Biophysical techniques
EV Enriched markers
✘
EV Negative markers
✘
EV Particle analysis
Identified molecule
protein
Identification method
Western blotting
PubMed ID
26061593
Organism
Homo sapiens
Experiment description
Pathogenesis of pancreatic cancer exosome-induced lipolysis in adipose tissue
Authors
Sagar G, Sah RP, Javeed N, Dutta SK, Smyrk TC, Lau JS, Giorgadze N, Tchkonia T, Kirkland JL, Chari ST, Mukhopadhyay D.
Journal name
Gut
Publication year
2017
Sample
Pancreatic cancer cells
Sample name
PANC-1 - Adrenomedullin immunoprecipitation
Isolation/purification methods
Differential centrifugation Ultracentrifugation
Flotation density
-
Molecules identified in the study
Protein
Methods used in the study
Western blotting
4
Experiment ID
953
MISEV standards
✘
EV Biophysical techniques
EV Enriched markers
✘
EV Negative markers
✘
EV Particle analysis
Identified molecule
protein
Identification method
Western blotting
PubMed ID
26061593
Organism
Homo sapiens
Experiment description
Pathogenesis of pancreatic cancer exosome-induced lipolysis in adipose tissue
Authors
Sagar G, Sah RP, Javeed N, Dutta SK, Smyrk TC, Lau JS, Giorgadze N, Tchkonia T, Kirkland JL, Chari ST, Mukhopadhyay D.
Journal name
Gut
Publication year
2017
Sample
Pancreatic cancer cells
Sample name
6741-1 (MCPAN014) - Adrenomedullin immunoprecipitation
Isolation/purification methods
Differential centrifugation Ultracentrifugation
Flotation density
-
Molecules identified in the study
Protein
Methods used in the study
Western blotting
5
Experiment ID
954
MISEV standards
✘
EV Biophysical techniques
EV Enriched markers
✘
EV Negative markers
✘
EV Particle analysis
Identified molecule
protein
Identification method
Western blotting
PubMed ID
26061593
Organism
Homo sapiens
Experiment description
Pathogenesis of pancreatic cancer exosome-induced lipolysis in adipose tissue
Authors
Sagar G, Sah RP, Javeed N, Dutta SK, Smyrk TC, Lau JS, Giorgadze N, Tchkonia T, Kirkland JL, Chari ST, Mukhopadhyay D.
Journal name
Gut
Publication year
2017
Sample
Pancreatic cancer cells
Sample name
6741-1 (MCPAN014) - Adrenomedullin immunoprecipitation
Isolation/purification methods
Differential centrifugation Ultracentrifugation
Flotation density
-
Molecules identified in the study
Protein
Methods used in the study
Western blotting
6
Experiment ID
949
MISEV standards
✘
EV Biophysical techniques
✔
CD63|TSG101
EV Enriched markers
✘
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule
protein
Identification method
Western blotting
PubMed ID
26061593
Organism
Homo sapiens
Experiment description
Pathogenesis of pancreatic cancer exosome-induced lipolysis in adipose tissue
Authors
Sagar G, Sah RP, Javeed N, Dutta SK, Smyrk TC, Lau JS, Giorgadze N, Tchkonia T, Kirkland JL, Chari ST, Mukhopadhyay D.
Journal name
Gut
Publication year
2017
Sample
Plasma
Sample name
Non-pancreatic cancer control
Isolation/purification methods
Differential centrifugation Ultracentrifugation
Flotation density
-
Molecules identified in the study
Protein
Methods used in the study
Western blotting
7
Experiment ID
950
MISEV standards
✘
EV Biophysical techniques
✔
CD63|TSG101
EV Enriched markers
✘
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule
protein
Identification method
Western blotting
PubMed ID
26061593
Organism
Homo sapiens
Experiment description
Pathogenesis of pancreatic cancer exosome-induced lipolysis in adipose tissue
Authors
Sagar G, Sah RP, Javeed N, Dutta SK, Smyrk TC, Lau JS, Giorgadze N, Tchkonia T, Kirkland JL, Chari ST, Mukhopadhyay D.
Journal name
Gut
Publication year
2017
Sample
Plasma
Sample name
Pancreatic cancer patients
Isolation/purification methods
Differential centrifugation Ultracentrifugation
Flotation density
-
Molecules identified in the study
Protein
Methods used in the study
Western blotting
Protein-protein interactions for ADM
Protein Interactor
ExoCarta ID
Identification method
PubMed
Species
1
ADMR
Invivo
Homo sapiens
2
CFH
3075
Invitro
Homo sapiens
3
EDN1
Invivo
Homo sapiens
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Pathways in which ADM is involved