Gene description for Ehd1
Gene name EH-domain containing 1
Gene symbol Ehd1
Other names/aliases AA409636
Past1
RME-1
Species Mus musculus
 Database cross references - Ehd1
ExoCarta ExoCarta_13660
Entrez Gene 13660
UniProt Q9WVK4  
 Ehd1 identified in exosomes derived from the following tissue/cell type
Embryonic fibroblasts 18494037    
Fibroblasts 23260141    
Macrophages 23658846    
Macrophages 23658846    
Macrophages 23658846    
 Gene ontology annotations for Ehd1
Molecular Function
    metal ion binding GO:0046872 IEA
    calcium ion binding GO:0005509 IEA
    ATP binding GO:0005524 IEA
    Rab GTPase binding GO:0017137 ISO
    GTP binding GO:0005525 IEA
    protein binding GO:0005515 IPI
    nucleotide binding GO:0000166 IEA
Biological Process
    cholesterol homeostasis GO:0042632 IMP
    neuron projection development GO:0031175 ISO
    positive regulation of endocytic recycling GO:2001137 IMP
    intracellular protein transport GO:0006886 ISO
    cellular response to nerve growth factor stimulus GO:1990090 ISO
    protein homooligomerization GO:0051260 ISO
    positive regulation of cholesterol storage GO:0010886 IMP
    endocytic recycling GO:0032456 ISO
    endocytosis GO:0006897 ISO
    low-density lipoprotein particle clearance GO:0034383 IMP
    positive regulation of myoblast fusion GO:1901741 IMP
    endosomal transport GO:0016197 IDA
Subcellular Localization
    myelin sheath GO:0043209 IDA
    extracellular exosome GO:0070062 ISO
    early endosome membrane GO:0031901 ISO
    platelet dense tubular network membrane GO:0031095 IDA
    endosome GO:0005768 IDA
    endocytic vesicle GO:0030139 IDA
    lipid particle GO:0005811 IDA
    early endosome GO:0005769 IDA
    endosome membrane GO:0010008 ISO
    perinuclear region of cytoplasm GO:0048471 IDA
    membrane GO:0016020 ISO
    recycling endosome membrane GO:0055038 ISO
    plasma membrane GO:0005886 IDA
 Experiment description of studies that identified Ehd1 in exosomes
1
Experiment ID 73
ISEV standards
EM
EV Biophysical techniques
HSP70|HSP90
EV Cytosolic markers
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 18494037    
Organism Mus musculus
Experiment description Difference gel electrophoresis analysis of Ras-transformed fibroblast cell-derived exosomes.
Authors Ji H, Erfani N, Tauro BJ, Kapp EA, Zhu HJ, Moritz RL, Lim JW, Simpson RJ
Journal name ELEC
Publication year 2008
Sample Embryonic fibroblasts
Sample name NIH3T3
Isolation/purification methods Differential centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [LCQ DECA]
Western blotting
2
Experiment ID 210
ISEV standards
EM
EV Biophysical techniques
EV Cytosolic markers
CD81|FLOT1
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23260141    
Organism Mus musculus
Experiment description Exosomes Mediate Stromal Mobilization of Autocrine Wnt-PCP Signaling in Breast Cancer Cell Migration.
Authors Luga V, Zhang L, Viloria-Petit AM, Ogunjimi AA, Inanlou MR, Chiu E, Buchanan M, Hosein AN, Basik M, Wrana JL.
Journal name Cell
Publication year 2012
Sample Fibroblasts
Sample name Normal-Fibroblasts (L cells)
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
3
Experiment ID 214
ISEV standards
EM
EV Biophysical techniques
EV Cytosolic markers
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23658846    
Organism Mus musculus
Experiment description Immunomodulatory impact of leishmania-induced macrophage exosomes: a comparative proteomic and functional analysis.
Authors Hassani K, Olivier M.
Journal name PLoS Negl Trop Dis
Publication year 2013
Sample Macrophages
Sample name Leishmania-infected-Macrophage (J774A.1)
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Filtration
Protease inhibitors
Sucrose density gradient
Filtration
Ultracentrifugation
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
4
Experiment ID 215
ISEV standards
EM
EV Biophysical techniques
EV Cytosolic markers
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23658846    
Organism Mus musculus
Experiment description Immunomodulatory impact of leishmania-induced macrophage exosomes: a comparative proteomic and functional analysis.
Authors Hassani K, Olivier M.
Journal name PLoS Negl Trop Dis
Publication year 2013
Sample Macrophages
Sample name LPS-treated-Macrophage (J774A.1)
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Filtration
Protease inhibitors
Sucrose density gradient
Filtration
Ultracentrifugation
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
5
Experiment ID 216
ISEV standards
EM
EV Biophysical techniques
EV Cytosolic markers
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23658846    
Organism Mus musculus
Experiment description Immunomodulatory impact of leishmania-induced macrophage exosomes: a comparative proteomic and functional analysis.
Authors Hassani K, Olivier M.
Journal name PLoS Negl Trop Dis
Publication year 2013
Sample Macrophages
Sample name Normal-Macrophage (J774A.1)
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Filtration
Protease inhibitors
Sucrose density gradient
Filtration
Ultracentrifugation
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for Ehd1
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.
 Pathways in which Ehd1 is involved
PathwayEvidenceSource
Factors involved in megakaryocyte development and platelet production IEA Reactome





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