Gene description for Eif4g2
Gene name eukaryotic translation initiation factor 4, gamma 2
Gene symbol Eif4g2
Other names/aliases AA589388
DAP-5
E130105L11Rik
Nat1
Natm1
p97
Species Mus musculus
 Database cross references - Eif4g2
ExoCarta ExoCarta_13690
Vesiclepedia VP_13690
Entrez Gene 13690
UniProt Q62448  
 Eif4g2 identified in sEVs derived from the following tissue/cell type
Colon cancer cells 37309723    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Eif4g2
Molecular Function
    RNA binding GO:0003723 IEA
    mRNA binding GO:0003729 IBA
    translation initiation factor activity GO:0003743 IBA
    translation initiation factor activity GO:0003743 IDA
    translation initiation factor activity GO:0003743 ISO
    translation initiation factor activity GO:0003743 ISS
    protein binding GO:0005515 IPI
    translation factor activity, RNA binding GO:0008135 ISO
    translation factor activity, RNA binding GO:0008135 ISS
Biological Process
    translational initiation GO:0006413 IBA
    translational initiation GO:0006413 IEA
    regulation of translational initiation GO:0006446 IDA
    regulation of translational initiation GO:0006446 ISO
    regulation of translational initiation GO:0006446 ISS
    macromolecule biosynthetic process GO:0009059 ISO
    negative regulation of autophagy GO:0010507 ISO
    positive regulation of cell growth GO:0030307 ISO
    positive regulation of translation GO:0045727 ISO
    positive regulation of axon extension GO:0045773 ISO
    positive regulation of dendritic spine development GO:0060999 ISO
Subcellular Localization
    cytoplasm GO:0005737 IDA
    cytosol GO:0005829 ISO
    postsynaptic density GO:0014069 EXP
    postsynaptic density GO:0014069 IDA
    eukaryotic translation initiation factor 4F complex GO:0016281 IBA
    eukaryotic translation initiation factor 4F complex GO:0016281 ISO
    eukaryotic translation initiation factor 4F complex GO:0016281 ISS
    axon GO:0030424 ISO
 Experiment description of studies that identified Eif4g2 in sEVs
1
Experiment ID 907
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 908
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Eif4g2
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Atg16l1  
Affinity Capture-MS Mus musculus
2 Kctd13  
Affinity Capture-MS Mus musculus
3 Tmem173  
Proximity Label-MS Mus musculus
4 Unk  
Affinity Capture-RNA Mus musculus
View the network image/svg+xml



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