Gene description for CR1
Gene name complement component (3b/4b) receptor 1 (Knops blood group)
Gene symbol CR1
Other names/aliases C3BR
C4BR
CD35
KN
Species Homo sapiens
 Database cross references - CR1
ExoCarta ExoCarta_1378
Entrez Gene 1378
HGNC 2334
MIM 120620
UniProt P17927  
 CR1 identified in exosomes derived from the following tissue/cell type
Urine 15326289    
Urine 19056867    
Urine 22418980    
Urine 22418980    
 Gene ontology annotations for CR1
Molecular Function
    complement component C4b receptor activity GO:0001861 IDA
    complement component C4b binding GO:0001855 IDA
    complement component C3b receptor activity GO:0004877 IDA
    complement component C3b binding GO:0001851 IDA
Biological Process
    negative regulation of complement activation, classical pathway GO:0045959 IDA
    negative regulation of serine-type endopeptidase activity GO:1900004 IDA
    complement receptor mediated signaling pathway GO:0002430 IDA
    regulation of complement activation GO:0030449 TAS
    complement activation, classical pathway GO:0006958 IEA
    innate immune response GO:0045087 TAS
    negative regulation of complement activation, alternative pathway GO:0045957 IDA
    positive regulation of serine-type endopeptidase activity GO:1900005 IDA
Subcellular Localization
    cell surface GO:0009986 IDA
    plasma membrane GO:0005886 TAS
    integral component of plasma membrane GO:0005887 IDA
    extracellular exosome GO:0070062 IDA
 Experiment description of studies that identified CR1 in exosomes
1
Experiment ID 13
ISEV standards
✔
IEM
EV Biophysical techniques
✔
Alix|RAB4|RAB5B|RAB11|TSG101
EV Cytosolic markers
✔
CD9|AQP2|AQP1
EV Membrane markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 15326289    
Organism Homo sapiens
Experiment description Identification and proteomic profiling of exosomes in human urine.
Authors Pisitkun T, Shen RF, Knepper MA
Journal name PNAS
Publication year 2004
Sample Urine
Sample name Urine - Normal
Isolation/purification methods Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [LCQ DECA XP]
Western blotting
2
Experiment ID 63
ISEV standards
✘ EV Biophysical techniques
✘ EV Cytosolic markers
✔
AQP2
EV Membrane markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 19056867    
Organism Homo sapiens
Experiment description Large-scale proteomics and phosphoproteomics of urinary exosomes.
Authors Gonzales PA, Pisitkun T, Hoffert JD, Tchapyjnikov D, Star RA, Kleta R, Wang NS, Knepper MA
Journal name JASN
Publication year 2009
Sample Urine
Sample name Urine - Normal
Isolation/purification methods Differential centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [LTQ]
Western blotting
3
Experiment ID 196
ISEV standards
✔
EM
EV Biophysical techniques
✔
Alix|TSG101|HSP70
EV Cytosolic markers
✔
CD9
EV Membrane markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 22418980    
Organism Homo sapiens
Experiment description A multiplex quantitative proteomics strategy for protein biomarker studies in urinary exosomes.
Authors Raj DA, Fiume I, Capasso G, Pocsfalvi G.
Journal name Kidney Int
Publication year 2012
Sample Urine
Sample name Urine - Normal high density
Isolation/purification methods Differential centrifugation
Sucrose cushion
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
4
Experiment ID 197
ISEV standards
✔
EM
EV Biophysical techniques
✔
Alix|TSG101|HSP70
EV Cytosolic markers
✔
CD9
EV Membrane markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 22418980    
Organism Homo sapiens
Experiment description A multiplex quantitative proteomics strategy for protein biomarker studies in urinary exosomes.
Authors Raj DA, Fiume I, Capasso G, Pocsfalvi G.
Journal name Kidney Int
Publication year 2012
Sample Urine
Sample name Urine - Normal low density
Isolation/purification methods Differential centrifugation
Sucrose cushion
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for CR1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 C1QA 712
Invitro Homo sapiens
2 C4B  
Invitro Homo sapiens
3 CD55 1604
Invitro Homo sapiens
4 C4A  
Invitro Homo sapiens
5 CR2 1380
Invivo Homo sapiens
6 C3 718
Invivo Homo sapiens
Invitro Homo sapiens
7 FUT4  
Invivo Homo sapiens
View the network image/svg+xml
 Pathways in which CR1 is involved
PathwayEvidenceSource
Regulation of Complement cascade TAS Reactome





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