Gene description for Enpep
Gene name glutamyl aminopeptidase
Gene symbol Enpep
Other names/aliases 6030431M22Rik
APA
Bp-1/6C3
Ly-51
Ly51
Species Mus musculus
 Database cross references - Enpep
ExoCarta ExoCarta_13809
Vesiclepedia VP_13809
Entrez Gene 13809
UniProt P16406  
 Enpep identified in sEVs derived from the following tissue/cell type
Faeces 33431899    
Faeces 33431899    
Pancreatic cells 19351151    
 Gene ontology annotations for Enpep
Molecular Function
    aminopeptidase activity GO:0004177 ISO
    aminopeptidase activity GO:0004177 ISS
    peptidase activity GO:0008233 IDA
    metallopeptidase activity GO:0008237 IDA
    metallopeptidase activity GO:0008237 IMP
    zinc ion binding GO:0008270 IBA
    zinc ion binding GO:0008270 IEA
    peptide binding GO:0042277 IBA
    peptide binding GO:0042277 ISO
    metalloaminopeptidase activity GO:0070006 IBA
    metalloaminopeptidase activity GO:0070006 ISO
    metalloaminopeptidase activity GO:0070006 ISS
Biological Process
    angiogenesis GO:0001525 IMP
    angiotensin maturation GO:0002003 IDA
    angiotensin maturation GO:0002003 IMP
    regulation of systemic arterial blood pressure by renin-angiotensin GO:0003081 ISO
    regulation of systemic arterial blood pressure by renin-angiotensin GO:0003081 ISS
    proteolysis GO:0006508 IBA
    proteolysis GO:0006508 IEA
    regulation of blood pressure GO:0008217 IBA
    cell population proliferation GO:0008283 ISS
    cell migration GO:0016477 ISO
    cell migration GO:0016477 ISS
    protein processing GO:0016485 ISO
    glomerulus development GO:0032835 IEP
    peptide catabolic process GO:0043171 IBA
    peptide catabolic process GO:0043171 ISO
    vascular endothelial cell proliferation GO:0101023 ISO
Subcellular Localization
    extracellular space GO:0005615 IBA
    cytoplasm GO:0005737 IBA
    plasma membrane GO:0005886 IBA
    plasma membrane GO:0005886 ISO
    plasma membrane GO:0005886 ISS
    plasma membrane GO:0005886 TAS
    brush border GO:0005903 IDA
    external side of plasma membrane GO:0009897 IDA
    external side of plasma membrane GO:0009897 IDA
    apical plasma membrane GO:0016324 IDA
    cytoplasmic vesicle GO:0031410 IDA
    brush border membrane GO:0031526 ISO
    apical part of cell GO:0045177 IDA
 Experiment description of studies that identified Enpep in sEVs
1
Experiment ID 303
MISEV standards
EM
Biophysical techniques
Cd63|Cd81|Cd9
Enriched markers
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33431899    
Organism Mus musculus
Experiment description High-fat diet-induced upregulation of exosomal phosphatidylcholine contributes to insulin resistance
Authors "Kumar A, Sundaram K, Mu J, Dryden GW, Sriwastva MK, Lei C, Zhang L, Qiu X, Xu F, Yan J, Zhang X, Park JW, Merchant ML, Bohler HCL, Wang B, Zhang S, Qin C, Xu Z, Han X, McClain CJ, Teng Y, Zhang HG."
Journal name Nat Commun
Publication year 2021
Sample Faeces
Sample name Intestinal epithelial cells - C57BL/6
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient centrifugation
Flotation density -
Molecules identified in the study Protein
Lipid
miRNA
Methods used in the study Western blotting
Mass spectrometry
Immunofluorescence
miRNA array
HPLC
2
Experiment ID 304
MISEV standards
EM
Biophysical techniques
Cd63|Cd81|Cd9
Enriched markers
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33431899    
Organism Mus musculus
Experiment description High-fat diet-induced upregulation of exosomal phosphatidylcholine contributes to insulin resistance
Authors "Kumar A, Sundaram K, Mu J, Dryden GW, Sriwastva MK, Lei C, Zhang L, Qiu X, Xu F, Yan J, Zhang X, Park JW, Merchant ML, Bohler HCL, Wang B, Zhang S, Qin C, Xu Z, Han X, McClain CJ, Teng Y, Zhang HG."
Journal name Nat Commun
Publication year 2021
Sample Faeces
Sample name Intestinal epithelial cells - C57BL/6
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient centrifugation
Flotation density -
Molecules identified in the study Protein
Lipid
miRNA
Methods used in the study Western blotting
Mass spectrometry
Immunofluorescence
miRNA array
HPLC
3
Experiment ID 188
MISEV standards
EM
Biophysical techniques
GAPDH|UCHL1|HSP90
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 19351151    
Organism Mus musculus
Experiment description Characterization of vesicles secreted from insulinoma NIT-1 cells.
Authors "Lee HS, Jeong J, Lee KJ."
Journal name J Proteome Res
Publication year 2009
Sample Pancreatic cells
Sample name Pancreatic beta cell (NIT-1)
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for Enpep
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.
 Pathways in which Enpep is involved
PathwayEvidenceSource
Metabolism of Angiotensinogen to Angiotensins IEA Reactome
Metabolism of proteins IEA Reactome
Peptide hormone metabolism IEA Reactome





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