Gene description for Ephb2
Gene name Eph receptor B2
Gene symbol Ephb2
Other names/aliases Cek5
Drt
ETECK
Erk
Hek5
Nuk
Prkm5
Qek5
Sek3
Tyro5
Species Mus musculus
 Database cross references - Ephb2
ExoCarta ExoCarta_13844
Vesiclepedia VP_13844
Entrez Gene 13844
 Ephb2 identified in sEVs derived from the following tissue/cell type
Colon cancer cells 37309723    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Ephb2
Molecular Function
    amyloid-beta binding GO:0001540 IPI
    protein tyrosine kinase activity GO:0004713 IDA
    protein tyrosine kinase activity GO:0004713 IMP
    ephrin receptor activity GO:0005003 IDA
    GPI-linked ephrin receptor activity GO:0005004 IEA
    transmembrane-ephrin receptor activity GO:0005005 IBA
    transmembrane-ephrin receptor activity GO:0005005 IDA
    transmembrane-ephrin receptor activity GO:0005005 TAS
    epidermal growth factor receptor activity GO:0005006 IEA
    fibroblast growth factor receptor activity GO:0005007 IEA
    hepatocyte growth factor receptor activity GO:0005008 IEA
    insulin receptor activity GO:0005009 IEA
    insulin-like growth factor receptor activity GO:0005010 IEA
    macrophage colony-stimulating factor receptor activity GO:0005011 IEA
    platelet-derived growth factor alpha-receptor activity GO:0005018 IEA
    platelet-derived growth factor beta-receptor activity GO:0005019 IEA
    stem cell factor receptor activity GO:0005020 IEA
    vascular endothelial growth factor receptor activity GO:0005021 IEA
    signaling receptor binding GO:0005102 IPI
    protein binding GO:0005515 IPI
    ATP binding GO:0005524 IEA
    axon guidance receptor activity GO:0008046 IDA
    boss receptor activity GO:0008288 IEA
    histone H3Y41 kinase activity GO:0035401 IEA
    placental growth factor receptor activity GO:0036332 IEA
    signaling receptor activity GO:0038023 IDA
    protein tyrosine kinase collagen receptor activity GO:0038062 IEA
    identical protein binding GO:0042802 IPI
    protein-containing complex binding GO:0044877 IPI
    protein-containing complex binding GO:0044877 ISO
    brain-derived neurotrophic factor receptor activity GO:0060175 IEA
    histone H2AXY142 kinase activity GO:0140801 IEA
Biological Process
    cell morphogenesis GO:0000902 IGI
    angiogenesis GO:0001525 IMP
    urogenital system development GO:0001655 IMP
    negative regulation of protein phosphorylation GO:0001933 IDA
    positive regulation of immunoglobulin production GO:0002639 IEA
    positive regulation of immunoglobulin production GO:0002639 ISO
    chromatin remodeling GO:0006338 IEA
    protein phosphorylation GO:0006468 IDA
    negative regulation of protein kinase activity GO:0006469 IDA
    negative regulation of cell adhesion GO:0007162 IEA
    negative regulation of cell adhesion GO:0007162 ISO
    cell surface receptor protein tyrosine kinase signaling pathway GO:0007169 IDA
    epidermal growth factor receptor signaling pathway GO:0007173 IEA
    axonogenesis GO:0007409 IDA
    axon guidance GO:0007411 IBA
    axon guidance GO:0007411 IDA
    axon guidance GO:0007411 IMP
    axonal fasciculation GO:0007413 IMP
    learning or memory GO:0007611 IGI
    learning GO:0007612 IMP
    insulin receptor signaling pathway GO:0008286 IEA
    fibroblast growth factor receptor signaling pathway GO:0008543 IEA
    animal organ morphogenesis GO:0009887 IMP
    positive regulation of gene expression GO:0010628 ISO
    phosphorylation GO:0016310 IDA
    peptidyl-tyrosine phosphorylation GO:0018108 IDA
    peptidyl-tyrosine phosphorylation GO:0018108 IDA
    peptidyl-tyrosine phosphorylation GO:0018108 IMP
    optic nerve morphogenesis GO:0021631 IMP
    hindbrain tangential cell migration GO:0021934 IEA
    hindbrain tangential cell migration GO:0021934 ISO
    central nervous system projection neuron axonogenesis GO:0021952 IMP
    corpus callosum development GO:0022038 IMP
    regulation of blood coagulation GO:0030193 ISO
    regulation of blood coagulation GO:0030193 ISS
    positive regulation of cell migration GO:0030335 IEA
    positive regulation of cell migration GO:0030335 ISO
    positive regulation of B cell proliferation GO:0030890 IEA
    positive regulation of B cell proliferation GO:0030890 ISO
    retinal ganglion cell axon guidance GO:0031290 IDA
    brain-derived neurotrophic factor receptor signaling pathway GO:0031547 IEA
    positive regulation of synaptic plasticity GO:0031915 IGI
    positive regulation of tumor necrosis factor production GO:0032760 IEA
    positive regulation of tumor necrosis factor production GO:0032760 ISO
    platelet-derived growth factor receptor-alpha signaling pathway GO:0035790 IEA
    platelet-derived growth factor receptor-beta signaling pathway GO:0035791 IEA
    vascular endothelial growth factor receptor-1 signaling pathway GO:0036323 IEA
    collagen-activated tyrosine kinase receptor signaling pathway GO:0038063 IEA
    vascular endothelial growth factor signaling pathway GO:0038084 IEA
    Kit signaling pathway GO:0038109 IEA
    macrophage colony-stimulating factor signaling pathway GO:0038145 IEA
    B cell activation GO:0042113 IEA
    B cell activation GO:0042113 ISO
    inner ear morphogenesis GO:0042472 IMP
    regulation of receptor signaling pathway via JAK-STAT GO:0046425 IEA
    regulation of receptor signaling pathway via JAK-STAT GO:0046425 ISO
    negative regulation of Ras protein signal transduction GO:0046580 IDA
    insulin-like growth factor receptor signaling pathway GO:0048009 IEA
    hepatocyte growth factor receptor signaling pathway GO:0048012 IEA
    ephrin receptor signaling pathway GO:0048013 IBA
    ephrin receptor signaling pathway GO:0048013 IDA
    ephrin receptor signaling pathway GO:0048013 IDA
    regulation of neuronal synaptic plasticity GO:0048168 IMP
    positive regulation of long-term neuronal synaptic plasticity GO:0048170 IMP
    camera-type eye morphogenesis GO:0048593 IMP
    regulation of axonogenesis GO:0050770 IDA
    negative regulation of axonogenesis GO:0050771 IDA
    regulation of body fluid levels GO:0050878 IMP
    regulation of filopodium assembly GO:0051489 IEA
    regulation of filopodium assembly GO:0051489 ISO
    regulation of synapse assembly GO:0051963 EXP
    regulation of synapse assembly GO:0051963 IDA
    regulation of synapse assembly GO:0051963 IMP
    positive regulation of synapse assembly GO:0051965 IDA
    positive regulation of synapse assembly GO:0051965 IMP
    positive regulation of synapse assembly GO:0051965 ISO
    roof of mouth development GO:0060021 IMP
    dendritic spine development GO:0060996 IMP
    dendritic spine morphogenesis GO:0060997 IDA
    dendritic spine morphogenesis GO:0060997 IMP
    positive regulation of dendritic spine morphogenesis GO:0061003 IEA
    positive regulation of dendritic spine morphogenesis GO:0061003 ISO
    negative regulation of ERK1 and ERK2 cascade GO:0070373 IDA
    cellular response to lipopolysaccharide GO:0071222 IEA
    commissural neuron axon guidance GO:0071679 IMP
    postsynaptic membrane assembly GO:0097104 IEA
    postsynaptic membrane assembly GO:0097104 ISO
    neuron projection retraction GO:0106028 IDA
    vesicle-mediated intercellular transport GO:0110077 IEA
    vesicle-mediated intercellular transport GO:0110077 ISO
    tight junction assembly GO:0120192 IEA
    tight junction assembly GO:0120192 ISO
    negative regulation of cytokine production involved in inflammatory response GO:1900016 IEA
    negative regulation of cytokine production involved in inflammatory response GO:1900016 ISO
    positive regulation of long-term synaptic potentiation GO:1900273 IMP
    positive regulation of protein localization to plasma membrane GO:1903078 ISO
    cellular response to amyloid-beta GO:1904646 IEA
    negative regulation of NMDA glutamate receptor activity GO:1904782 IMP
    positive regulation of NMDA glutamate receptor activity GO:1904783 IMP
    positive regulation of protein localization to cell surface GO:2000010 IEA
    positive regulation of protein localization to cell surface GO:2000010 ISO
    regulation of T-helper 17 type immune response GO:2000316 IEA
    regulation of T-helper 17 type immune response GO:2000316 ISO
    regulation of autophagosome assembly GO:2000785 IEA
    regulation of autophagosome assembly GO:2000785 ISO
    regulation of behavioral fear response GO:2000822 IEA
    regulation of behavioral fear response GO:2000822 ISO
Subcellular Localization
    nucleoplasm GO:0005654 IEA
    nucleoplasm GO:0005654 ISO
    plasma membrane GO:0005886 IBA
    plasma membrane GO:0005886 IDA
    plasma membrane GO:0005886 ISO
    plasma membrane GO:0005886 TAS
    cell surface GO:0009986 IEA
    cell surface GO:0009986 ISO
    axon GO:0030424 IDA
    dendrite GO:0030425 IBA
    dendrite GO:0030425 IDA
    dendrite GO:0030425 ISO
    presynaptic membrane GO:0042734 IDA
    presynaptic membrane GO:0042734 IEP
    neuronal cell body GO:0043025 IDA
    neuronal cell body GO:0043025 ISO
    dendritic spine GO:0043197 IEA
    dendritic spine GO:0043197 ISO
    synapse GO:0045202 NAS
    postsynaptic membrane GO:0045211 IDA
    postsynaptic membrane GO:0045211 IEP
    hippocampal mossy fiber to CA3 synapse GO:0098686 IDA
    postsynapse GO:0098794 ISO
    glutamatergic synapse GO:0098978 EXP
    glutamatergic synapse GO:0098978 IDA
    glutamatergic synapse GO:0098978 IEP
    glutamatergic synapse GO:0098978 IMP
    glutamatergic synapse GO:0098978 ISO
 Experiment description of studies that identified Ephb2 in sEVs
1
Experiment ID 907
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 908
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Ephb2
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Elf2  
Affinity Capture-Luminescence Mus musculus
Affinity Capture-Luminescence Mus musculus
2 Sstr3  
Proximity Label-MS Mus musculus
3 Ryk  
Affinity Capture-Western Mus musculus
4 Efnb1  
Affinity Capture-Luminescence Mus musculus
Affinity Capture-Luminescence Mus musculus
5 Arhgef15  
Affinity Capture-Western Mus musculus
Affinity Capture-Western Mus musculus
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