Gene description for Ephb4
Gene name Eph receptor B4
Gene symbol Ephb4
Other names/aliases AI042935
Htk
MDK2
Myk1
Tyro11
Species Mus musculus
 Database cross references - Ephb4
ExoCarta ExoCarta_13846
Vesiclepedia VP_13846
Entrez Gene 13846
UniProt P54761  
 Ephb4 identified in sEVs derived from the following tissue/cell type
Colon cancer cells 37309723    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Ephb4
Molecular Function
    transmembrane receptor protein tyrosine kinase activity GO:0004714 IBA
    transmembrane receptor protein tyrosine kinase activity GO:0004714 ISO
    transmembrane receptor protein tyrosine kinase activity GO:0004714 ISS
    ephrin receptor activity GO:0005003 ISO
    ephrin receptor activity GO:0005003 ISS
    GPI-linked ephrin receptor activity GO:0005004 IEA
    transmembrane-ephrin receptor activity GO:0005005 IEA
    epidermal growth factor receptor activity GO:0005006 IEA
    fibroblast growth factor receptor activity GO:0005007 IEA
    hepatocyte growth factor receptor activity GO:0005008 IEA
    insulin receptor activity GO:0005009 IEA
    insulin-like growth factor receptor activity GO:0005010 IEA
    macrophage colony-stimulating factor receptor activity GO:0005011 IEA
    platelet-derived growth factor alpha-receptor activity GO:0005018 IEA
    platelet-derived growth factor beta-receptor activity GO:0005019 IEA
    stem cell factor receptor activity GO:0005020 IEA
    vascular endothelial growth factor receptor activity GO:0005021 IEA
    protein binding GO:0005515 IPI
    ATP binding GO:0005524 IEA
    boss receptor activity GO:0008288 IEA
    histone H3Y41 kinase activity GO:0035401 IEA
    placental growth factor receptor activity GO:0036332 IEA
    protein tyrosine kinase collagen receptor activity GO:0038062 IEA
    brain-derived neurotrophic factor receptor activity GO:0060175 IEA
    histone H2AXY142 kinase activity GO:0140801 IEA
Biological Process
    angiogenesis GO:0001525 IBA
    angiogenesis GO:0001525 IMP
    cell migration involved in sprouting angiogenesis GO:0002042 ISO
    cell migration involved in sprouting angiogenesis GO:0002042 ISS
    heart morphogenesis GO:0003007 IMP
    chromatin remodeling GO:0006338 IEA
    cell adhesion GO:0007155 ISO
    cell adhesion GO:0007155 ISS
    epidermal growth factor receptor signaling pathway GO:0007173 IEA
    multicellular organism development GO:0007275 IBA
    insulin receptor signaling pathway GO:0008286 IEA
    fibroblast growth factor receptor signaling pathway GO:0008543 IEA
    brain-derived neurotrophic factor receptor signaling pathway GO:0031547 IEA
    platelet-derived growth factor receptor-alpha signaling pathway GO:0035790 IEA
    platelet-derived growth factor receptor-beta signaling pathway GO:0035791 IEA
    vascular endothelial growth factor receptor-1 signaling pathway GO:0036323 IEA
    collagen-activated tyrosine kinase receptor signaling pathway GO:0038063 IEA
    vascular endothelial growth factor signaling pathway GO:0038084 IEA
    Kit signaling pathway GO:0038109 IEA
    macrophage colony-stimulating factor signaling pathway GO:0038145 IEA
    protein autophosphorylation GO:0046777 ISO
    protein autophosphorylation GO:0046777 ISS
    insulin-like growth factor receptor signaling pathway GO:0048009 IEA
    hepatocyte growth factor receptor signaling pathway GO:0048012 IEA
    ephrin receptor signaling pathway GO:0048013 IBA
    ephrin receptor signaling pathway GO:0048013 IGI
    ephrin receptor signaling pathway GO:0048013 ISO
    venous blood vessel morphogenesis GO:0048845 IMP
    positive regulation of aorta morphogenesis GO:1903849 IMP
    positive regulation of T cell costimulation GO:2000525 IGI
Subcellular Localization
    plasma membrane GO:0005886 IBA
    plasma membrane GO:0005886 ISO
    plasma membrane GO:0005886 ISS
    receptor complex GO:0043235 IBA
 Experiment description of studies that identified Ephb4 in sEVs
1
Experiment ID 907
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 908
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Ephb4
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Epha8  
Co-fractionation Mus musculus
2 Erbb3  
Co-fractionation Mus musculus
3 Ddr1  
Co-fractionation Mus musculus
4 Elf2  
Affinity Capture-Luminescence Mus musculus
5 Elf1  
Affinity Capture-Luminescence Mus musculus
Co-fractionation Mus musculus
6 Ephb3  
Co-fractionation Mus musculus
7 Bmx  
Co-fractionation Mus musculus
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