Gene description for Ephx1
Gene name epoxide hydrolase 1, microsomal
Gene symbol Ephx1
Other names/aliases AI195553
Eph-1
Eph1
mEH
Species Mus musculus
 Database cross references - Ephx1
ExoCarta ExoCarta_13849
Vesiclepedia VP_13849
Entrez Gene 13849
UniProt Q9D379  
 Ephx1 identified in sEVs derived from the following tissue/cell type
Colon cancer cells 37309723    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Ephx1
Molecular Function
    epoxide hydrolase activity GO:0004301 IBA
    epoxide hydrolase activity GO:0004301 ISO
    epoxide hydrolase activity GO:0004301 ISS
    oxysterol binding GO:0008142 IMP
    enzyme binding GO:0019899 ISO
    cis-stilbene-oxide hydrolase activity GO:0033961 ISO
    cis-stilbene-oxide hydrolase activity GO:0033961 ISS
Biological Process
    response to toxic substance GO:0009636 IEA
    arachidonate metabolic process GO:0019369 IBA
    arachidonate metabolic process GO:0019369 ISO
    arachidonate metabolic process GO:0019369 ISS
    diol biosynthetic process GO:0034312 ISO
    epoxide metabolic process GO:0097176 IBA
    epoxide metabolic process GO:0097176 IEA
    epoxide metabolic process GO:0097176 ISO
    hydrocarbon catabolic process GO:0120253 IMP
    response to polycyclic arene GO:1903165 IMP
Subcellular Localization
    endoplasmic reticulum membrane GO:0005789 IEA
    membrane GO:0016020 ISO
    intracellular membrane-bounded organelle GO:0043231 ISO
 Experiment description of studies that identified Ephx1 in sEVs
1
Experiment ID 907
MISEV standards
EM
Biophysical techniques
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
HSPA5
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 908
MISEV standards
EM
Biophysical techniques
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
HSPA5
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Ephx1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Tmem173  
Proximity Label-MS Mus musculus
View the network image/svg+xml
 Pathways in which Ephx1 is involved
PathwayEvidenceSource
Biological oxidations IEA Reactome
Metabolism IEA Reactome
Phase I - Functionalization of compounds IEA Reactome





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