Gene description for Epn2
Gene name epsin 2
Gene symbol Epn2
Other names/aliases 9530051D10Rik
AA536924
Ibp2
Species Mus musculus
 Database cross references - Epn2
ExoCarta ExoCarta_13855
Vesiclepedia VP_13855
Entrez Gene 13855
UniProt Q8CHU3  
 Epn2 identified in exosomes derived from the following tissue/cell type
Melanoma cells 34957415    
Melanoma cells 34957415    
 Gene ontology annotations for Epn2
Molecular Function
    phospholipid binding GO:0005543 IBA
    lipid binding GO:0008289 IEA
    clathrin binding GO:0030276 IBA
Biological Process
    in utero embryonic development GO:0001701 IGI
    endocytosis GO:0006897 IBA
    endocytosis GO:0006897 IEA
    Notch signaling pathway GO:0007219 IGI
    regulation of endocytosis GO:0030100 ISO
    negative regulation of vascular endothelial growth factor receptor signaling pathway GO:0030948 ISO
    positive regulation of Notch signaling pathway GO:0045747 ISO
    embryonic organ development GO:0048568 IGI
    negative regulation of sprouting angiogenesis GO:1903671 ISO
Subcellular Localization
    cytoplasm GO:0005737 IEA
    endosome GO:0005768 IBA
    plasma membrane GO:0005886 IBA
    clathrin vesicle coat GO:0030125 IBA
    clathrin coat of endocytic vesicle GO:0030128 ISO
    intracellular membrane-bounded organelle GO:0043231 ISO
 Experiment description of studies that identified Epn2 in exosomes
1
Experiment ID 1117
MISEV standards
Biophysical techniques
Cd81|Icam1
Enriched markers
Negative markers
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 34957415    
Organism Mus musculus
Experiment description Melanoma-derived small extracellular vesicles induce lymphangiogenesis and metastasis through an NGFR-dependent mechanism
Authors "García-Silva S, Benito-Martín A, Nogués L, Hernández-Barranco A, Mazariegos MS, Santos V, Hergueta-Redondo M, Ximénez-Embún P, Kataru RP, Lopez AA, Merino C, Sánchez-Redondo S, Graña-Castro O, Matei I, Nicolás-Avila JÁ, Torres-Ruiz R, Rodríguez-Perales S, Martínez L, Pérez-Martínez M, Mata G, Szumera-Ciećkiewicz A, Kalinowska I, Saltari A, Martínez-Gómez JM, Hogan SA, Saragovi HU, Ortega S, Garcia-Martin C, Boskovic J, Levesque MP, Rutkowski P, Hidalgo A, Muñoz J, Megías D, Mehrara BJ, Lyden D, Peinado H."
Journal name Nat Cancer
Publication year 2021
Sample Melanoma cells
Sample name B16-F1R2
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 1118
MISEV standards
Biophysical techniques
Cd81|Icam1
Enriched markers
Negative markers
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 34957415    
Organism Mus musculus
Experiment description Melanoma-derived small extracellular vesicles induce lymphangiogenesis and metastasis through an NGFR-dependent mechanism
Authors "García-Silva S, Benito-Martín A, Nogués L, Hernández-Barranco A, Mazariegos MS, Santos V, Hergueta-Redondo M, Ximénez-Embún P, Kataru RP, Lopez AA, Merino C, Sánchez-Redondo S, Graña-Castro O, Matei I, Nicolás-Avila JÁ, Torres-Ruiz R, Rodríguez-Perales S, Martínez L, Pérez-Martínez M, Mata G, Szumera-Ciećkiewicz A, Kalinowska I, Saltari A, Martínez-Gómez JM, Hogan SA, Saragovi HU, Ortega S, Garcia-Martin C, Boskovic J, Levesque MP, Rutkowski P, Hidalgo A, Muñoz J, Megías D, Mehrara BJ, Lyden D, Peinado H."
Journal name Nat Cancer
Publication year 2021
Sample Melanoma cells
Sample name B16-F1R2
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Epn2
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Ralbp1  
Co-fractionation Mus musculus
2 Kctd13  
Affinity Capture-MS Mus musculus
3 Ubc  
Reconstituted Complex Mus musculus
Reconstituted Complex Mus musculus
4 Msr1  
Affinity Capture-Western Mus musculus
5 Eed  
Affinity Capture-MS Mus musculus
6 Flt4  
Affinity Capture-Western Mus musculus
7 ITSN1 6453
Two-hybrid Homo sapiens
View the network image/svg+xml



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