Gene description for F5
Gene name coagulation factor V
Gene symbol F5
Other names/aliases AI173222
Cf-5
Cf5
Species Mus musculus
 Database cross references - F5
ExoCarta ExoCarta_14067
Entrez Gene 14067
UniProt O88783  
 F5 identified in exosomes derived from the following tissue/cell type
Macrophages 23658846    
Macrophages 23658846    
Macrophages 23658846    
 Gene ontology annotations for F5
Molecular Function
    serine-type endopeptidase activity GO:0004252 ISO
    metal ion binding GO:0046872 IEA
    copper ion binding GO:0005507 IEA
Biological Process
    platelet activation GO:0030168 IEA
    blood coagulation GO:0007596 ISO
    proteolysis GO:0006508 ISO
    blood circulation GO:0008015 IMP
    hemostasis GO:0007599 IEA
Subcellular Localization
    platelet alpha granule GO:0031091 IDA
    extracellular region GO:0005576 ISO
    Golgi apparatus GO:0005794 ISO
    membrane GO:0016020 ISO
    extracellular space GO:0005615 IDA
    extracellular vesicle GO:1903561 ISO
    endoplasmic reticulum GO:0005783 ISO
 Experiment description of studies that identified F5 in exosomes
1
Experiment ID 214
ISEV standards
EM
EV Biophysical techniques
EV Cytosolic markers
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23658846    
Organism Mus musculus
Experiment description Immunomodulatory impact of leishmania-induced macrophage exosomes: a comparative proteomic and functional analysis.
Authors Hassani K, Olivier M.
Journal name PLoS Negl Trop Dis
Publication year 2013
Sample Macrophages
Sample name Leishmania-infected-Macrophage (J774A.1)
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Filtration
Protease inhibitors
Sucrose density gradient
Filtration
Ultracentrifugation
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
2
Experiment ID 215
ISEV standards
EM
EV Biophysical techniques
EV Cytosolic markers
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23658846    
Organism Mus musculus
Experiment description Immunomodulatory impact of leishmania-induced macrophage exosomes: a comparative proteomic and functional analysis.
Authors Hassani K, Olivier M.
Journal name PLoS Negl Trop Dis
Publication year 2013
Sample Macrophages
Sample name LPS-treated-Macrophage (J774A.1)
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Filtration
Protease inhibitors
Sucrose density gradient
Filtration
Ultracentrifugation
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
3
Experiment ID 216
ISEV standards
EM
EV Biophysical techniques
EV Cytosolic markers
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23658846    
Organism Mus musculus
Experiment description Immunomodulatory impact of leishmania-induced macrophage exosomes: a comparative proteomic and functional analysis.
Authors Hassani K, Olivier M.
Journal name PLoS Negl Trop Dis
Publication year 2013
Sample Macrophages
Sample name Normal-Macrophage (J774A.1)
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Filtration
Protease inhibitors
Sucrose density gradient
Filtration
Ultracentrifugation
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for F5
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.
 Pathways in which F5 is involved
PathwayEvidenceSource
Common Pathway of Fibrin Clot Formation IEA Reactome
Platelet degranulation IEA Reactome





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