ExoCarta: Gene summary

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Gene description for Hnrnph1
Gene name	heterogeneous nuclear ribonucleoprotein H1
Gene symbol	Hnrnph1
Other names/aliases	Hnrph Hnrph1
Species	Rattus norvegicus
Database cross references - Hnrnph1
ExoCarta	ExoCarta_140931
Entrez Gene	140931
UniProt	Q8VHV7
Hnrnph1 identified in exosomes derived from the following tissue/cell type
Reticulocytes	21828046
Reticulocytes	21828046

Gene ontology annotations for Hnrnph1

Molecular Function
	enzyme binding	GO:0019899	IPI
	nucleic acid binding	GO:0003676	IEA
	RNA binding	GO:0003723	ISS
	nucleotide binding	GO:0000166	IEA
	poly(A) RNA binding	GO:0044822	ISO
Biological Process
	RNA processing	GO:0006396	TAS
	mRNA processing	GO:0006397	IEA
	regulation of RNA splicing	GO:0043484	ISS
	RNA splicing	GO:0008380	IEA
Subcellular Localization
	catalytic step 2 spliceosome	GO:0071013	ISO
	membrane	GO:0016020	ISO
	nucleus	GO:0005634	IDA
	nucleoplasm	GO:0005654	IDA
	viral nucleocapsid	GO:0019013	IEA
	spliceosomal complex	GO:0005681	IEA

Experiment description of studies that identified Hnrnph1 in exosomes

Experiment ID

ISEV standards
✔ EM	EV Biophysical techniques
✔ TSG101\|HSP90\|HSC70	EV Cytosolic markers
✔ MHCI	EV Membrane markers
✘	EV Negative markers
✘	EV Particle analysis

Identified molecule

protein

Identification method

Mass spectrometry [Orbitrap]

PubMed ID

21828046

Organism

Rattus norvegicus

Experiment description

The proteo-lipidic composition of exosomes changes during reticulocyte maturation.

Authors

Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E.

Journal name

JBC

Publication year

2011

Sample

Reticulocytes

Sample name

Reticulocytes - D4 Rep 1

Isolation/purification methods

Differential centrifugation
Ultracentrifugation
Sucrose density gradient

Flotation density

1.16-1.21 g/mL

Molecules identified in the study

Protein
Lipids

Methods used in the study

Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting