ExoCarta: Gene summary

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Gene description for Ikbkap
Gene name	inhibitor of kappa light polypeptide gene enhancer in B-cells, kinase complex-associated protein
Gene symbol	Ikbkap
Other names/aliases	-
Species	Rattus norvegicus
Database cross references - Ikbkap
ExoCarta	ExoCarta_140934
Entrez Gene	140934
UniProt	Q8VHU4
Ikbkap identified in exosomes derived from the following tissue/cell type
Reticulocytes	21828046
Reticulocytes	21828046

Gene ontology annotations for Ikbkap

Molecular Function
	RNA polymerase II core binding	GO:0000993	ISO
	ATP binding	GO:0005524	IDA
	protein kinase activity	GO:0004672	IDA
	kinase activity	GO:0016301	IEA
	phosphorylase kinase regulator activity	GO:0008607	ISS
Biological Process
	transcription, DNA-templated	GO:0006351	IEA
	regulation of protein kinase activity	GO:0045859	ISS
	regulation of transcription from RNA polymerase II promoter	GO:0006357	ISS
	I-kappaB phosphorylation	GO:0007252	IDA
	phosphorylation	GO:0016310	IEA
	positive regulation of cell migration	GO:0030335	ISS
Subcellular Localization
	nucleolus	GO:0005730	ISO
	histone acetyltransferase complex	GO:0000123	ISO
	transcription elongation factor complex	GO:0008023	ISS
	cellular_component	GO:0005575	ND
	cytoplasm	GO:0005737	ISS
	Elongator holoenzyme complex	GO:0033588	ISS

Experiment description of studies that identified Ikbkap in exosomes

Experiment ID

ISEV standards
✔ EM	EV Biophysical techniques
✔ TSG101\|HSP90\|HSC70	EV Cytosolic markers
✔ MHCI	EV Membrane markers
✘	EV Negative markers
✘	EV Particle analysis

Identified molecule

protein

Identification method

Mass spectrometry [Orbitrap]

PubMed ID

21828046

Organism

Rattus norvegicus

Experiment description

The proteo-lipidic composition of exosomes changes during reticulocyte maturation.

Authors

Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E.

Journal name

JBC

Publication year

2011

Sample

Reticulocytes

Sample name

Reticulocytes - D4 Rep 2

Isolation/purification methods

Differential centrifugation
Ultracentrifugation
Sucrose density gradient

Flotation density

1.16-1.21 g/mL

Molecules identified in the study

Protein
Lipids

Methods used in the study

Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting