Gene description for Ikbkap
Gene name inhibitor of kappa light polypeptide gene enhancer in B-cells, kinase complex-associated protein
Gene symbol Ikbkap
Other names/aliases -
Species Rattus norvegicus
 Database cross references - Ikbkap
ExoCarta ExoCarta_140934
Vesiclepedia VP_140934
Entrez Gene 140934
UniProt Q8VHU4  
 Ikbkap identified in sEVs derived from the following tissue/cell type
Reticulocytes 21828046    
Reticulocytes 21828046    
 Gene ontology annotations for Ikbkap
Molecular Function
    tRNA binding GO:0000049 IBA
    protein kinase activity GO:0004672 IDA
    ATP binding GO:0005524 IDA
Biological Process
    tRNA wobble uridine modification GO:0002098 IEA
    tRNA wobble uridine modification GO:0002098 ISO
    tRNA wobble base 5-methoxycarbonylmethyl-2-thiouridinylation GO:0002926 IBA
    I-kappaB phosphorylation GO:0007252 IDA
Subcellular Localization
    nucleus GO:0005634 IEA
    cytoplasm GO:0005737 ISO
    cytoplasm GO:0005737 ISS
    cytosol GO:0005829 IBA
    cytosol GO:0005829 IEA
    cytosol GO:0005829 ISO
    elongator holoenzyme complex GO:0033588 IBA
    elongator holoenzyme complex GO:0033588 IEA
    elongator holoenzyme complex GO:0033588 ISO
    elongator holoenzyme complex GO:0033588 ISS
 Experiment description of studies that identified Ikbkap in sEVs
1
Experiment ID 97
MISEV standards
EM
Biophysical techniques
TSG101|HSP90|HSC70|MHCI
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors "Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E."
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D4 Rep 2
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.16-1.21 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
2
Experiment ID 98
MISEV standards
EM
Biophysical techniques
TSG101|HSP90|HSC70|MHCI
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors "Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E."
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D7 Rep 2
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.25 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
 Protein-protein interactions for Ikbkap
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.
 Pathways in which Ikbkap is involved
No pathways found





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