Gene description for Srsf10
Gene name serine/arginine-rich splicing factor 10
Gene symbol Srsf10
Other names/aliases FUSIP2
Fusip1
NSSR1
NSSR2
Nssr
SRrp40
Sfrs13a
Srsf13a
TASR
TASR1
TASR2
Species Mus musculus
 Database cross references - Srsf10
ExoCarta ExoCarta_14105
Vesiclepedia VP_14105
Entrez Gene 14105
UniProt Q9R0U0  
 Srsf10 identified in sEVs derived from the following tissue/cell type
Colon cancer cells 37309723    
Mast cells 17486113    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Srsf10
Molecular Function
    RNA binding GO:0003723 IBA
    RNA binding GO:0003723 ISO
    RNA binding GO:0003723 ISS
    RNA binding GO:0003723 NAS
    unfolded protein binding GO:0051082 NAS
Biological Process
    RNA splicing, via transesterification reactions GO:0000375 IDA
    RNA splicing, via transesterification reactions GO:0000375 ISO
    mRNA splicing, via spliceosome GO:0000398 IBA
    mRNA splicing, via spliceosome GO:0000398 ISO
    regulation of DNA-templated transcription GO:0006355 NAS
    mRNA splice site recognition GO:0006376 IC
    mRNA splice site recognition GO:0006376 ISO
    mRNA splice site recognition GO:0006376 ISS
    cytosolic transport GO:0016482 IDA
    regulation of mRNA splicing, via spliceosome GO:0048024 ISO
    negative regulation of mRNA splicing, via spliceosome GO:0048025 IDA
    negative regulation of mRNA splicing, via spliceosome GO:0048025 ISO
Subcellular Localization
    nucleoplasm GO:0005654 IDA
    nucleoplasm GO:0005654 ISO
    cytosol GO:0005829 IEA
    nuclear speck GO:0016607 IBA
    nuclear speck GO:0016607 ISO
    nuclear speck GO:0016607 ISS
    dendrite GO:0030425 IEA
    dendrite GO:0030425 ISO
    neuronal cell body GO:0043025 IEA
    neuronal cell body GO:0043025 ISO
    axon terminus GO:0043679 IEA
    axon terminus GO:0043679 ISO
 Experiment description of studies that identified Srsf10 in sEVs
1
Experiment ID 907
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 15
MISEV standards
✔
EM
Biophysical techniques
✔
CD63
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule mrna
Identification method Microarray
PubMed ID 17486113    
Organism Mus musculus
Homo sapiens
Experiment description Exosome-mediated transfer of mRNAs and microRNAs is a novel mechanism of genetic exchange between cells.
Authors "Valadi H, Ekstrom K, Bossios A, Sjostrand M, Lee JJ, Lotvall JO"
Journal name NCB
Publication year 2007
Sample Mast cells
Sample name MC9
Bone marrow-derived mast cells
HMC-1
Isolation/purification methods Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.11-1.21 g/mL
Molecules identified in the study Protein
mRNA
miRNA
Methods used in the study Mass spectrometry [MALDI TOF]
Western blotting
Microarray
miRCURY LNA Array
3
Experiment ID 908
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Srsf10
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Fancd2  
Affinity Capture-MS Mus musculus
2 Atxn1  
Affinity Capture-MS Mus musculus
3 Eed  
Affinity Capture-MS Mus musculus
4 Fus  
Affinity Capture-Western Mus musculus
Two-hybrid Mus musculus
View the network image/svg+xml



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