|
 Gene description for Galnt1 |
| Gene name |
UDP-N-acetyl-alpha-D-galactosamine:polypeptide N-acetylgalactosaminyltransferase 1 |
| Gene symbol |
Galnt1 |
| Other names/aliases |
- |
| Species |
Mus musculus |
| Database cross references - Galnt1 |
| ExoCarta |
ExoCarta_14423 |
| Vesiclepedia |
VP_14423 |
| Entrez Gene |
14423 |
| UniProt |
O08912
|
| Galnt1 identified in sEVs derived from the following tissue/cell type |
|
Colon cancer cells
|
37309723
|
|
Mast cells
|
17486113
|
|
T-cell lymphoma cells
|
37309723
|
| Gene ontology annotations for Galnt1 |
|
|
| Experiment description of studies that identified Galnt1 in sEVs |
| 1 |
| Experiment ID |
907 |
| MISEV standards |
|
✔
EM
|
Biophysical techniques |
|
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
|
Enriched markers |
|
✔
HSPA5
|
Negative markers |
|
✔
NTA
|
Particle analysis
|
|
| Identified molecule |
Protein
|
| Identification method |
Mass spectrometry
|
| PubMed ID |
37309723
|
| Organism |
Mus musculus |
| Experiment description |
Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells |
| Authors |
"Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S." |
| Journal name |
Proteomics
|
| Publication year |
2023 |
| Sample |
Colon cancer cells |
| Sample name |
C26 |
| Isolation/purification methods |
Differential centrifugation
Ultracentrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Western blotting
Mass spectrometry |
|
|
| 2 |
| Experiment ID |
15 |
| MISEV standards |
|
✔
EM
|
Biophysical techniques |
|
✔
CD63
|
Enriched markers |
|
✘
|
Negative markers |
|
✘
|
Particle analysis
|
|
| Identified molecule |
mrna
|
| Identification method |
Microarray
|
| PubMed ID |
17486113
|
| Organism |
Mus musculus
Homo sapiens |
| Experiment description |
Exosome-mediated transfer of mRNAs and microRNAs is a novel mechanism of genetic exchange between cells. |
| Authors |
"Valadi H, Ekstrom K, Bossios A, Sjostrand M, Lee JJ, Lotvall JO" |
| Journal name |
NCB
|
| Publication year |
2007 |
| Sample |
Mast cells |
| Sample name |
MC9
Bone marrow-derived mast cells
HMC-1 |
| Isolation/purification methods |
Filtration
Ultracentrifugation
Sucrose density gradient |
| Flotation density |
1.11-1.21 g/mL
|
| Molecules identified in the study |
Protein
mRNA
miRNA |
| Methods used in the study |
Mass spectrometry [MALDI TOF]
Western blotting
Microarray
miRCURY LNA Array |
|
|
| 3 |
| Experiment ID |
908 |
| MISEV standards |
|
✔
EM
|
Biophysical techniques |
|
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
|
Enriched markers |
|
✔
HSPA5
|
Negative markers |
|
✔
NTA
|
Particle analysis
|
|
| Identified molecule |
Protein
|
| Identification method |
Mass spectrometry
|
| PubMed ID |
37309723
|
| Organism |
Mus musculus |
| Experiment description |
Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells |
| Authors |
"Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S." |
| Journal name |
Proteomics
|
| Publication year |
2023 |
| Sample |
T-cell lymphoma cells |
| Sample name |
EL4 |
| Isolation/purification methods |
Differential centrifugation
Ultracentrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Western blotting
Mass spectrometry |
|
|
| Protein-protein interactions for Galnt1 |
|
|
| Pathways in which Galnt1 is involved |
|
|
|
