Gene description for Galnt1
Gene name UDP-N-acetyl-alpha-D-galactosamine:polypeptide N-acetylgalactosaminyltransferase 1
Gene symbol Galnt1
Other names/aliases -
Species Mus musculus
 Database cross references - Galnt1
ExoCarta ExoCarta_14423
Vesiclepedia VP_14423
Entrez Gene 14423
UniProt O08912  
 Galnt1 identified in exosomes derived from the following tissue/cell type
Colon cancer cells 37309723    
Mast cells 17486113    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Galnt1
Molecular Function
    metal ion binding GO:0046872 IEA
    transferase activity, transferring glycosyl groups GO:0016757 IEA
    transferase activity GO:0016740 IEA
    carbohydrate binding GO:0030246 IEA
    polypeptide N-acetylgalactosaminyltransferase activity GO:0004653 ISO
    manganese ion binding GO:0030145 ISO
Biological Process
    protein O-linked glycosylation GO:0006493 IDA
    protein O-linked glycosylation via serine GO:0018242 ISO
    protein O-linked glycosylation via threonine GO:0018243 ISO
Subcellular Localization
    integral component of membrane GO:0016021 IEA
    Golgi apparatus GO:0005794 ISO
    membrane GO:0016020 ISO
    extracellular region GO:0005576 IEA
    perinuclear region of cytoplasm GO:0048471 ISO
 Experiment description of studies that identified Galnt1 in exosomes
1
Experiment ID 907
MISEV standards
EM
EV Biophysical techniques
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
EV Enriched markers
HSPA5
EV Negative markers
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S.
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 15
MISEV standards
EM
EV Biophysical techniques
CD63
EV Enriched markers
EV Negative markers
EV Particle analysis
Identified molecule mrna
Identification method Microarray
PubMed ID 17486113    
Organism Mus musculus
Homo sapiens
Experiment description Exosome-mediated transfer of mRNAs and microRNAs is a novel mechanism of genetic exchange between cells.
Authors Valadi H, Ekstrom K, Bossios A, Sjostrand M, Lee JJ, Lotvall JO
Journal name NCB
Publication year 2007
Sample Mast cells
Sample name MC9
Bone marrow-derived mast cells
HMC-1
Isolation/purification methods Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.11-1.21 g/mL
Molecules identified in the study Protein
mRNA
miRNA
Methods used in the study Mass spectrometry [MALDI TOF]
Western blotting
Microarray
miRCURY LNA Array
3
Experiment ID 908
MISEV standards
EM
EV Biophysical techniques
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
EV Enriched markers
HSPA5
EV Negative markers
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S.
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Galnt1
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.
 Pathways in which Galnt1 is involved
PathwayEvidenceSource
O-linked glycosylation of mucins IEA Reactome





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