Gene description for Gnaq
Gene name guanine nucleotide binding protein, alpha q polypeptide
Gene symbol Gnaq
Other names/aliases 1110005L02Rik
6230401I02Rik
AA408290
AW060788
Dsk1
Dsk10
Galphaq
Gq
GqI
Species Mus musculus
 Database cross references - Gnaq
ExoCarta ExoCarta_14682
Vesiclepedia VP_14682
Entrez Gene 14682
UniProt P21279  
 Gnaq identified in exosomes derived from the following tissue/cell type
Colon cancer cells 37309723    
Embryonic fibroblasts 18494037    
Mov neuroglial cells 15210972    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Gnaq
Molecular Function
    GTPase activity GO:0003924 TAS
    metal ion binding GO:0046872 IEA
    protein binding GO:0005515 IPI
    G-protein coupled receptor binding GO:0001664 IEA
    signal transducer activity GO:0004871 IBA
    GTP binding GO:0005525 ISO
    nucleotide binding GO:0000166 IEA
    protein complex binding GO:0032403 ISO
    GTPase activator activity GO:0005096 ISO
    type 2A serotonin receptor binding GO:0031826 ISO
    G-protein beta/gamma-subunit complex binding GO:0031683 IBA
    guanyl nucleotide binding GO:0019001 IEA
Biological Process
    negative regulation of protein kinase activity GO:0006469 ISO
    adenylate cyclase-activating G-protein coupled receptor signaling pathway GO:0007189 IDA
    embryonic digit morphogenesis GO:0042733 IGI
    forebrain neuron development GO:0021884 IMP
    glutamate receptor signaling pathway GO:0007215 IMP
    heart development GO:0007507 IMP
    negative regulation of potassium ion transport GO:0043267 ISO
    phospholipase C-activating G-protein coupled receptor signaling pathway GO:0007200 ISO
    action potential GO:0001508 IMP
    activation of phospholipase C activity GO:0007202 ISO
    developmental pigmentation GO:0048066 IMP
    post-embryonic development GO:0009791 IMP
    neuron remodeling GO:0016322 IMP
    protein stabilization GO:0050821 ISO
    signal transduction GO:0007165 IBA
    G-protein coupled receptor signaling pathway GO:0007186 ISO
    skeletal system development GO:0001501 IMP
    maternal behavior GO:0042711 IMP
    regulation of catenin import into nucleus GO:0035412 ISO
    negative regulation of apoptotic process GO:0043066 ISO
    regulation of melanocyte differentiation GO:0045634 IMP
    positive regulation of smooth muscle cell proliferation GO:0048661 ISO
    positive regulation of GTPase activity GO:0043547 ISO
    phospholipase C-activating dopamine receptor signaling pathway GO:0060158 IGI
Subcellular Localization
    cytosol GO:0005829 ISO
    extracellular exosome GO:0070062 ISO
    caveola GO:0005901 ISO
    lysosomal membrane GO:0005765 ISO
    plasma membrane GO:0005886 ISO
    nucleus GO:0005634 IEA
    protein complex GO:0043234 ISO
    membrane GO:0016020 ISO
    heterotrimeric G-protein complex GO:0005834 TAS
 Experiment description of studies that identified Gnaq in exosomes
1
Experiment ID 907
MISEV standards
✔
EM
EV Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
EV Enriched markers
✔
HSPA5
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S.
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 73
MISEV standards
✔
EM
EV Biophysical techniques
✔
HSP70|HSP90
EV Enriched markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 18494037    
Organism Mus musculus
Experiment description Difference gel electrophoresis analysis of Ras-transformed fibroblast cell-derived exosomes.
Authors Ji H, Erfani N, Tauro BJ, Kapp EA, Zhu HJ, Moritz RL, Lim JW, Simpson RJ
Journal name ELEC
Publication year 2008
Sample Embryonic fibroblasts
Sample name NIH3T3
Isolation/purification methods Differential centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [LCQ DECA]
Western blotting
3
Experiment ID 39
MISEV standards
✔
IEM
EV Biophysical techniques
✔
TSG101|HSC70|FLOT1
EV Enriched markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 15210972    
Organism Mus musculus
Experiment description Cells release prions in association with exosomes.
Authors Fevrier B, Vilette D, Archer F, Loew D, Faigle W, Vidal M, Laude H, Raposo G
Journal name PNAS
Publication year 2004
Sample Mov neuroglial cells
Sample name Mov neuroglial cell
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Flotation density 1.14 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [QSTAR]
Western blotting
Immunoelectron Microscopy
4
Experiment ID 908
MISEV standards
✔
EM
EV Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
EV Enriched markers
✔
HSPA5
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S.
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Gnaq
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.



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