Experiment description of studies that identified Hist1h1d in exosomes

1 Experiment ID 15 ISEV standards ✔ EM EV Biophysical techniques ✘ EV Cytosolic markers ✔ CD63 EV Membrane markers ✘ EV Negative markers ✘ EV Particle analysis Identified molecule protein Identification method Mass spectrometry PubMed ID 17486113     Organism Mus musculus Homo sapiens Experiment description Exosome-mediated transfer of mRNAs and microRNAs is a novel mechanism of genetic exchange between cells. Authors Valadi H, Ekstrom K, Bossios A, Sjostrand M, Lee JJ, Lotvall JO Journal name NCB Publication year 2007 Sample Mast cells Sample name MC9 Bone marrow-derived mast cells HMC-1 Isolation/purification methods Filtration Ultracentrifugation Sucrose density gradient Flotation density 1.11-1.21 g/mL Molecules identified in the study Protein mRNA miRNA Methods used in the study Mass spectrometry [MALDI TOF] Western blotting Microarray miRCURY LNA Array

2 Experiment ID 263 ISEV standards ✘ EV Biophysical techniques ✘ EV Cytosolic markers ✔ CD63|CD9 EV Membrane markers ✘ EV Negative markers ✘ EV Particle analysis Identified molecule protein Identification method Mass spectrometry PubMed ID 25242146     Organism Mus musculus Experiment description Extracellular Vesicles from Neural Stem Cells Transfer IFN-γ via Ifngr1 to Activate Stat1 Signaling in Target Cells Authors Chiara Cossetti, Nunzio Iraci, Tim R. Mercer, Tommaso Leonardi, Emanuele Alpi, Denise Drago, Clara Alfaro-Cervello, Harpreet K. Saini, Matthew P. Davis, Julia Schaeffer, Beatriz Vega, Matilde Stefanini, CongJian Zhao, Werner Muller, Jose Manuel Garcia-Verdugo, Suresh Mathivanan, Angela Bachi, Anton J. Enright, John S. Mattick, Stefano Pluchino Journal name Molecular Cell Publication year 2014 Sample Neural stem cells Sample name NPCs - Th1 treated Isolation/purification methods Differential centrifugation Ultracentrifugation Sucrose density gradient Flotation density 1.13-1.20 g/mL Molecules identified in the study Protein Methods used in the study Western blotting Mass spectrometry

3 Experiment ID 264 ISEV standards ✘ EV Biophysical techniques ✘ EV Cytosolic markers ✔ CD63|CD9 EV Membrane markers ✘ EV Negative markers ✘ EV Particle analysis Identified molecule protein Identification method Mass spectrometry PubMed ID 25242146     Organism Mus musculus Experiment description Extracellular Vesicles from Neural Stem Cells Transfer IFN-γ via Ifngr1 to Activate Stat1 Signaling in Target Cells Authors Chiara Cossetti, Nunzio Iraci, Tim R. Mercer, Tommaso Leonardi, Emanuele Alpi, Denise Drago, Clara Alfaro-Cervello, Harpreet K. Saini, Matthew P. Davis, Julia Schaeffer, Beatriz Vega, Matilde Stefanini, CongJian Zhao, Werner Muller, Jose Manuel Garcia-Verdugo, Suresh Mathivanan, Angela Bachi, Anton J. Enright, John S. Mattick, Stefano Pluchino Journal name Molecular Cell Publication year 2014 Sample Neural stem cells Sample name NPCs - Th2 treated Isolation/purification methods Differential centrifugation Ultracentrifugation Sucrose density gradient Flotation density 1.13-1.20 g/mL Molecules identified in the study Protein Methods used in the study Western blotting Mass spectrometry

4 Experiment ID 188 ISEV standards ✔ EM EV Biophysical techniques ✔ GAPDH|UCHL1|HSP90 EV Cytosolic markers ✘ EV Membrane markers ✘ EV Negative markers ✘ EV Particle analysis Identified molecule protein Identification method Mass spectrometry PubMed ID 19351151     Organism Mus musculus Experiment description Characterization of vesicles secreted from insulinoma NIT-1 cells. Authors Lee HS, Jeong J, Lee KJ. Journal name J Proteome Res Publication year 2009 Sample Pancreatic cells Sample name Pancreatic beta cell (NIT-1) Isolation/purification methods Differential centrifugation Sucrose density gradient Flotation density - Molecules identified in the study Protein Methods used in the study Mass spectrometry

Protein-protein interactions for Hist1h1d

Protein Interactor ExoCarta ID Identification method PubMed Species No interactions are found.

Pathways in which Hist1h1d is involved

No pathways found