Experiment ID |
3 |
ISEV standards |
✔
IEM
|
EV Biophysical techniques |
✔
HSP84|HSC73
|
EV Cytosolic markers |
✔
MHCII|CD9|mac-1
|
EV Membrane markers |
✔
CANX|HSP90B1
|
EV Negative markers |
✘
|
EV Particle analysis
|
|
Identified molecule |
protein
|
Identification method |
Mass spectrometry Western blotting
|
PubMed ID |
10545503
|
Organism |
Mus musculus |
Experiment description |
Molecular characterization of dendritic cell-derived exosomes. Selective accumulation of the heat shock protein hsc73. |
Authors |
Thery C, Regnault A, Garin J, Wolfers J, Zitvogel L, Ricciardi-Castagnoli P, Raposo G, Amigorena S. |
Journal name |
JCB
|
Publication year |
1999 |
Sample |
Dendritic cells |
Sample name |
D1 |
Isolation/purification methods |
Differential centrifugation Sucrose density gradient |
Flotation density |
1.14-1.20 g/mL
|
Molecules identified in the study |
Protein |
Methods used in the study |
Mass spectrometry [MALDI TOF] Western blotting |